Mycological Progress

, 14:39

Taxonomy and phylogenetic position of species of Amanita sect. Vaginatae s.l. from tropical Africa

  • L. P. Tang
  • Q. Cai
  • S. S. Lee
  • B. Buyck
  • P. Zhang
  • Zhu L. Yang
Original Article


The genus Amanita is well represented in tropical Africa, but most previous taxonomic studies were largely based only on morphology. In this study, we used both morphological and molecular data (nLSU sequences) to infer the phylogenetic relationships of ten species of Amanita sect. Vaginatae s.l. from tropical Africa. It was found that all five species with both an annulus and commonly present clamp connections in the basidioma were clustered in the sect. Caesareae. One species with neither an annulus nor clamp connections was placed in sect. Vaginatae s.s. The remaining four taxa with an annulus, but without clamp connections were interestingly also grouped into sect. Vaginatae s.s. with strong statistical support. Consequently, the section Vaginatae s.s. should be slightly modified to include tropical species with an annulus. As a new species, A. madagascariensis was described and illustrated. Amanita tanzanica was treated as a synonym of A. mafingensis.


Amanitaceae Classification Distribution New taxa Species diversity 


  1. Bas C (1969) Morphology and subdivision of Amanita and a monograph on its section Lepidella. Persoonia 5:285–579Google Scholar
  2. Bas C (1975) A comparison of Torrendia (Gasteromycetes) with Amanita (Agaricales). In: Bigelow HE, Thiers HD (eds) Studies on higher fungi. Beih. Nova Hedwigia, vol 51., pp 53–60Google Scholar
  3. Bas C (1982) Studies in Amanita − II. Persoonia 11:429–442Google Scholar
  4. Beeli M (1927) Contribution a l’étude de la flore mycologique du Congo. II. Bull Soc R Bot Belg 59:101–112Google Scholar
  5. Beeli M (1931) Contribution à l’étude de la flore mycologique du Congo. Fungi Goossensiani VIII. Genra Amanita Fr. Bull Soc R Bot Belg 63:100–109Google Scholar
  6. Beeli M (1935) Flore iconographique des champignons du Congo. III. Bull Soc R Bot Belg: 11–23Google Scholar
  7. Beeli M (1936) Contribution á l’etude de la flore mycologique du Congo. XI. Fungi Goossensiani XII. Bull Jard Bot Ėtat 14:83–91CrossRefGoogle Scholar
  8. Bouriquet G (1941) Quelques macromycètes de Madagascar. Bull Acad Malgache 24:61–64Google Scholar
  9. Bouriquet G (1943) Notes de mycologie malgache. Bull Acad Malgache 25:12–24Google Scholar
  10. Breitenbach J, Kränzlin F (1995) Pilze der Schweiz. Band 4. Verlag Mycologia, LuzernGoogle Scholar
  11. Buyck B (1994) Ubwoba: Les champignons comestibles de I’Ouest du Burundi. Adminstration Generale de la Cooperation au Developpement Bruxelles Publ. 34: 123, 84 figsGoogle Scholar
  12. Buyck B (2008) The edible mushrooms of Madagascar: an evolving enigma. Econ Bot 62:509–520CrossRefGoogle Scholar
  13. Cai Q, Tang LP, Yang ZL (2012) DNA barcoding of economically important mushrooms: a case study on lethal amanitas from China. Plant Divers Resour 34:614–622 (in Chinese)CrossRefGoogle Scholar
  14. Cai Q, Tulloss RE, Tang LP, Tolgor B, Zhang P, Chen ZH, Yang ZL (2014) Multi-locus phylogeny of lethal amanitas: implications for species diversity and historical biogeography. BMC Evol Biol 14:143PubMedCentralPubMedCrossRefGoogle Scholar
  15. Corner EJH, Bas C (1962) The genus Amanita in Singapore and Malaya. Persoonia 2:241–304Google Scholar
  16. Deng WQ, Li TH, Li P, Yang ZL (2014) A new species of Amanita section Lepidella from South China. Mycol Prog 13:211–217CrossRefGoogle Scholar
  17. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  18. Drehmel D, Moncalvo JM, Vilgalys R (1999) Molecular phylogeny of Amanita based on large-subunit ribosomal DNA sequences: implications for taxonomy and character evolution. Mycologia 91:610–618CrossRefGoogle Scholar
  19. Edgar RC (2004) Muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797PubMedCentralPubMedCrossRefGoogle Scholar
  20. Eicker A, Van Greuning JV, Reid DA (1993) Amanita reidii − A new species from South Africa. Mycotaxon 47:433–437Google Scholar
  21. Gilbert EJ (1940) Amanitaceae. In Bresadola J. Iconogr Mycol 27(Suppl 1):1–200Google Scholar
  22. Gilbert EJ (1941) Amanitaceae. In Bresadola J. Iconogr Mycol 27(Suppl 1):201–427Google Scholar
  23. Härkönen M, Saarimäki T, Mwasumbi L (1994) Tanzania mushrooms and their uses 4. Some reddish edible and poisonous Amanita species. Karstenia 34:47–60Google Scholar
  24. Huijsman HSC (1962a) [“1961”] Amanita beckeri nov. sp. Bull Soc Mycol Fr 77:349–350Google Scholar
  25. Huijsman HSC (1962b) Amanita beckeri nov. sp. (Diagnose latine). Bull Soc Mycol Fr 78:217Google Scholar
  26. Justo A, Morgenstern I, Hallen-Adams HE, Hibbett DS (2010) Convergent evolution of sequestrate forms in Amanita under Mediterranean climate conditions. Mycologia 102:675–688PubMedCrossRefGoogle Scholar
  27. Kornerup A, Wanscher JH (1981) Taschenlexikon der Farben. 3. Aufl. Muster-Schmidt Verlag, GöttingenGoogle Scholar
  28. Li F, Cai Q (2014) Amanita heishidingensis, a new species of Amanita sect. Lepidella from China. Mycol Prog 13:1191–1197CrossRefGoogle Scholar
  29. Miller JROK, Horak E (1992) Observations on the genus Torrendia and a new species from Australia. Mycologia 84:64–71CrossRefGoogle Scholar
  30. Pegler DN, Piearce GD (1980) The edible mushrooms of Zambia. Kew Bull 35:475–491CrossRefGoogle Scholar
  31. Pegler DN, Shah-Smith D (1997) The genus Amanita (Amanitaceae, Agaricales) in Zambia. Mycotaxon 61:389–417Google Scholar
  32. Phillips R (1990) Der Kosmos-Pilzatlas. Frackh-Kosmos Verlag, StuttgartGoogle Scholar
  33. Reid DA, Eicker A (1991) South African fungi: the genus Amanita. Mycol Res 95:80–95CrossRefGoogle Scholar
  34. Ronquist F, Huelsenbeck JP (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574PubMedCrossRefGoogle Scholar
  35. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol (Clifton, NJ) 132:365–86Google Scholar
  36. Sánchez-Ramírez S, Tulloss RE, Amalfi M, Moncalvo J-M (2015) Palaeotropical origins, boreotropical distribution and increased rates of diversification in a clade of edible ectomycorrhizal mushrooms (Amanita section Caesareae). J Biogeogr 42:351–363CrossRefGoogle Scholar
  37. Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web-servers. Syst Biol 75:758–771CrossRefGoogle Scholar
  38. Tedersoo L, Suvi T, Beaver K (2007) Ectomycorrhizal fungi of the Seychelles: diversity patterns and host shifts from native Vateriopsis seychellarum (Dipterocarpaceae) and Intsia bijuga (Caesalpiniaceae) to the introduced Eucalyptus robusta (Myrtaceae), but not Pinus caribaea (Pinaceae). New Phytol 175:321–333PubMedCrossRefGoogle Scholar
  39. Tulloss RE (1994) Type studies in Amanita section Vaginatae I: Some taxa described in this century (studies 1–23) with notes on description of spores and refractive hyphae in Amanita. Mycotaxon 52:305–396Google Scholar
  40. Tulloss RE, Possiel L (2008) Checklist of Amanita found in sub-Saharan Africa.
  41. Tulloss RE, Cl O, Halling RE (1992) Studies on Amanita (Amanitaceae) from Andean Colombia. Mem NY Bot Gard 66:1–46Google Scholar
  42. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246PubMedCentralPubMedGoogle Scholar
  43. Weiß M, Yang ZL, Oberwinkler F (1998) Molecular phylogenetic studies in the genus Amanita. Can J Bot 76:1170–1179Google Scholar
  44. Yang ZL (1997) Die Amanita-Arten von Südwestchina. Bibl Mycol 170:1–240Google Scholar
  45. Yang ZL (2004) Two new species of Amanita (Basidiomycota) from China. In: Agerer, Piepenbring, Blanz (eds) Frontiers in basidiomycote mycology. IHW-Verlag, Eching, pp 315–324Google Scholar
  46. Yang ZL (2005) Flora fungorum sinicorum”. Vol. 27 Amanitaceae. Science Press, BeijingGoogle Scholar
  47. Yang ZL (2011) Molecular techniques revolutionize knowledge of basidiomycete evolution. Fungal Divers 50:47–5CrossRefGoogle Scholar
  48. Yang ZL, Weiß M, Oberwinkler F (2004) New species of Amanita from the eastern Himalaya and adjacent regions. Mycologia 96:636–646PubMedCrossRefGoogle Scholar
  49. Zhang LF, Yang JB, Yang ZL (2004) Molecular phylogeny of eastern Asian species of Amanita (Agaricales, Basidiomycota): taxonomic and biogeographic implications. Fungal Divers 17:219–23Google Scholar
  50. Zhang P, Chen ZH, Xiao B, Tolgor B, Bao HY, Yang ZL (2010) Lethal amanitas of East Asia characterized by morphological and molecular data. Fungal Divers 42:119–133CrossRefGoogle Scholar

Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • L. P. Tang
    • 1
    • 2
  • Q. Cai
    • 1
    • 6
  • S. S. Lee
    • 3
  • B. Buyck
    • 4
  • P. Zhang
    • 5
  • Zhu L. Yang
    • 1
  1. 1.Key Laboratory for Plant Diversity and Biogeography of East AsiaKunming Institute of Botany, Chinese Academy of SciencesKunmingChina
  2. 2.School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural ProductsKunming Medical UniversityKunmingChina
  3. 3.Forest Research Institute MalaysiaKepongMalaysia
  4. 4.Département Systématique et ĖvolutionUMR7205, Muséum National d’Histoire NaturelleParisFrance
  5. 5.College of Life ScienceHunan Normal UniversityChangshaChina
  6. 6.University of Chinese Academy of SciencesBeijingChina

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