Mycological Progress

, 14:14 | Cite as

Inocybe flavobrunnescens, a new species in section Marginatae

  • Fernando Esteve-Raventós
  • Gabriel Moreno
  • Enrico Bizio
  • Pablo Alvarado
Original Article
First Online:
Received:
Revised:
Accepted:

Abstract

The new species Inocybe flavobrunnescens is described on the basis of morphological and genetic features. It is characterized by (sub)isodiametric spores; it has a variable number of low and obtuse knobs, lageniform cystidia, and habitat preference for thermophilous oak woods on calcareous soils. It is compared with type material of Inocybe xanthomelas and I. krieglsteineri, which share with the new species a darkening flesh and small to medium sized basidiomes. ITS analysis revealed a low intraspecific variability within each Inocybe lineage, suggesting their accommodation as independent species. On the basis of this DNA region, I. xanthomelas and I. krieglsteineri are closely related. The present work suggests also updating several names in public databases to accommodate the present taxonomic conclusions. A lectotype for I. xanthomelas is designated.

Keywords

Inocybe xanthomelas I. krieglsteineri I. urbana Taxonomy Type studies Molecular systematics Mediterranean Europe 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

First, we wish to express our gratitude to Dr. Javier Rejos, Curator at AH, for his permanent and invaluable help in requesting material and concern in our research; our colleague Dr. Ibai Olariaga for his kind help in obtaining some molecular sequences of this study: also to A. Priego and J.A. Pérez (Electron Microscopy Service at Alcalá University) for their assistance with the SEM work. We also thank Luis Monje and Angel Pueblas (Department of Drawing and Scientific Photography at Alcalá University) for their collaboration in the digital preparation of the photographs. Our deep gratitude is given to the Curators at G and LIP for the loan of type collections; lastly we appreciate the courtesy of Erminio Ferrari, Roberto Fernández-Sasia and Agustín Caballero, who sent us interesting collections for study.

References

  1. Alessio CL, Rebaudengo E (1980) Inocybe. Iconographia Mycologica Vol 29, Suppl 3. Museo Tridentino di Scienze Naturali, TrentoGoogle Scholar
  2. Alvarado P, Manjón JL, Matheny PB, Esteve-Raventós F (2010) Tubariomyces, a new genus of Inocybaceae from the Mediterranean region. Mycologia 102(6):1389–1397CrossRefPubMedGoogle Scholar
  3. Bandini D (2014) http://inocybe.org. Accessed 21 May 2014
  4. Bizio E (2009) Revisione critica del genere Inocybe (Cervicolores, Lactiferae, Cortinatae, Marginatae). In: Maire JC, Moreau PA, Robich G (eds) Compléments à la Flore des Champignons Supérieurs du Maroc de G. Malençon et R. Bertault. Confédération Européenne de Mycologie Méditerranéenne, Nice, pp 205–224Google Scholar
  5. Bon M (1998) Clé monographique du genre Inocybe (Fr.) Fr. (3ème partie: espèces gibbosporées = sous-genre Clypeus Britz., = Genre Astrosporina Schroet). Doc Mycol 28(111):1–45Google Scholar
  6. Bon M (1999) Étude de quelques Inocybes méridionaux aux Journées de la F.A.M.M. à Annot (Octobre 1998). Bull Fédér Assoc Mycol Médit 15:3–12Google Scholar
  7. Esteve-Raventós F (2004) Breves notas corológicas sobre algunos Agaricales recogidos en el Macizo Calcáreo Estremenho y Pinar de Leiria (Estremadura, Beira Litoral, Portugal). Bol Soc Micol Madrid 28:221–227Google Scholar
  8. Esteve-Raventós F, Caballero A (2009) Especies nuevas e interesantes del género Inocybe (Inocybaceae, Basidiomycetes) en la Comunidad de La Rioja (España Peninsular). Taxones con esporas nodulosas (I). Fungi non Delineati XLVII. Edizioni Candusso, AlassioGoogle Scholar
  9. Esteve-Raventós F, Muñoz G, Caballero A, Lainé P (2012) Inocybe urbana (Inocybaceae, Agaricales), primera cita para España y segunda mundial. Bol Micol FAMCAL 7:79–84Google Scholar
  10. Fernández Sasia R (2004) Inocybe krieglsteinerii sp. nov., une espèce encore peu connue. Bol Soc Mycol Fr 120:179–186Google Scholar
  11. Ferrari E (2006) Inocybe alpine e subalpine. Fungi non Delineati XXXIV-XXXV-XXXVI. Edizioni Candusso, AlassioGoogle Scholar
  12. Ferrari E (2010) Inocybe dai litorali alla zona alpina. Fungi non Delineati LIV-LV. Edizioni Candusso, AlassioGoogle Scholar
  13. Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118Google Scholar
  14. Heim R (1931) Le genre Inocybe précédé d’une introduction general à l’étude des Agarics ochrosporés. P Lechevalier, ParisGoogle Scholar
  15. Heinemann P, Rammeloo J (1985) De la mesure des spores et de son expression. Agarica 6(12):366–380Google Scholar
  16. Holmgren PK, Holmgren NH, Barnett LC (1990) Index Herbariorum, 8th edn. Botanic Garden, New YorkGoogle Scholar
  17. Jacobsson S, Larsson E (2012) Inocybe (Fr.) Fr. In: Knudsen H, Vesterholt J (eds) Funga Nordica (Agaricoid, boletoid, clavarioid, cyphelloid and gastroid genera). Nordsvamp, Copenhagen, pp 981–1021Google Scholar
  18. Jansen AE (1984) Vegetation and macrofungi of acid oakwoods in the northeast of the Netherlands. WageningenGoogle Scholar
  19. Kauffman CH (1924) Inocybe. N Am Fl 10:227–260Google Scholar
  20. Kobayashi T (2002) The taxonomic studies on the genus Inocybe. Nova Hedwigia Beih 124:1–246Google Scholar
  21. Krieglsteiner GJ (1989) Über neue, seltene, kritische Makromyzeten in der BR Deutschland (Mitteleuropa) XI. Beitr Kenntn Pilze Mitteleur 5:115–140Google Scholar
  22. Kühner R (1933) Notes sur le genre Inocybe: 1) Les Inocybes goniosporés (Fin). Bol Soc Mycol Fr 49:81–121Google Scholar
  23. Kühner R (1955) Compléments a la “Flore Analytique” VI) Inocybe goniosporés et Inocybe acystidiés. Espèces nouvelles ou critiques. Bull Soc Mycol Fr 71:169–201Google Scholar
  24. Kuyper TW (1986) A revision of the genus Inocybe in Europe. I. Subgenus Inosperma and the smooth-spored species of subgenus Inocybe. Persoonia Suppl 3:1–247Google Scholar
  25. Kuyper TW (1995) Inocybe (Fr.) Fr. – Vezelkop, Viltkop. In: Arnolds E, Kuyper TW, Noordeloos ME (eds) Overzicht van de paddestoelen in Nederland. Nederlandse Mycologische Vereniging, Wijster, pp 249–269Google Scholar
  26. Kuyper TW (2006) Begin eens met… Vezelkoppen (Inocybe) – 2: voorlopige determinatiesleutel tot de knobbelsporige vezelkoppen. Coolia 49:11–17Google Scholar
  27. Malençon G, Bertault R (1970) Flore des Champignons Supérieurs du Maroc, vol 1. Institut Scientifique Chérifien et Faculté des Sciences, RabatGoogle Scholar
  28. Marchetti M, Franchi P, Consiglio G (2014) Tipificazione di alcune Inocybe di Britzelmayr. Riv Micol 57(2):127–178Google Scholar
  29. Mühlmann O, Bacher M, Peintner U (2008) Polygonum viviparum mycobionts on an alpine primary successional glacier forefront. Mycorrhiza 18:87–95CrossRefPubMedGoogle Scholar
  30. Munsell® (1994) Munsell® soil color charts (revised edition). Macbeth Division of Kollmorgen Instruments Corporation, New WindsorGoogle Scholar
  31. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8(19):4321–4325CrossRefPubMedCentralPubMedGoogle Scholar
  32. Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Uppsala, Evolutionary Biology Centre, Uppsala University, SwedenGoogle Scholar
  33. Osmundson TW, Robert VA, Schoch CL, Baker LJ, Smith A, Robich G, Mizzan L, Garbelotto MM (2013) Filling gaps in biodiversity knowledge for macrofungi: contributions and assessment of an herbarium collection DNA barcode sequencing project. PLoS ONE 8(4):E62419CrossRefPubMedCentralPubMedGoogle Scholar
  34. Outen AR, Cullington P (2012) Keys to British species of Inocybe (2nd edition updated 2.2)Google Scholar
  35. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574CrossRefPubMedGoogle Scholar
  36. Ryberg M, Nilsson RH, Kristiansson E, Topel M, Jacobsson S, Larsson E (2008) Mining metadata from unidentified ITS sequences in GenBank: a case study in Inocybe (Basidiomycota). BMC Evol Biol 8:50CrossRefPubMedCentralPubMedGoogle Scholar
  37. Stamatakis A (2006) RAxML-VI-HPC: maximum likelihoodbased phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690CrossRefPubMedGoogle Scholar
  38. Stangl J (1989) Die Gattung Inocybe in Bayern. Hoppea 46:5–388Google Scholar
  39. Stuntz DE (1954) Studies in the genus Inocybe II. New and noteworthy species from Michigan. Pap Michigan Acad Sci Arts Lett 39:53–84Google Scholar
  40. Swofford DL (2001) PAUP*4.0b10: phylogenetic analysis using parsimony (and other methods). Sinauer Associates, SunderlandGoogle Scholar
  41. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739CrossRefPubMedCentralPubMedGoogle Scholar
  42. Vauras J, Kokkonen K (2009) Finnish records on the genus Inocybe. The new species Inocybe saliceticola. Karstenia 48:57–67Google Scholar
  43. Vellinga EC (1988) Glossary. In: Bas C, Kuyper TW, Noordeloos ME, Vellinga EC (eds) Flora Agaricina Neerlandica, vol 1. AA Balkema, Rotterdam, pp 54–64Google Scholar
  44. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplitication and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–332Google Scholar
  45. Zitzmann H (2002) Regensburger Pilzflora 12: Die Gattung Inocybe (Agaricales, Cortinariaceae). Regensb Mykol Schr 10:135–288Google Scholar

Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Fernando Esteve-Raventós
    • 1
  • Gabriel Moreno
    • 1
  • Enrico Bizio
    • 2
  • Pablo Alvarado
    • 3
  1. 1.Department of Life Sciences (Botany Unit)University of AlcaláAlcalá de HenaresSpain
  2. 2.Società Veneziana di Micologia, c/o Museo di Storia Naturale di Venezia, Fontego dei TurchiVeneziaItaly
  3. 3.ALVALABSantanderSpain

Personalised recommendations