Mycological Progress

, Volume 10, Issue 4, pp 415–422 | Cite as

Recent outbreaks of downy mildew on grape ivy (Parthenocissus tricuspidata, Vitaceae) in Germany are caused by a new species of Plasmopara

  • Marco ThinesEmail author
Original Article


Plasmopara viticola is one of the most destructive pathogens in grape production and was introduced from North America to Europe more than a century ago. The pathogen is thought to affect a broad range of hosts in the Vitaceae, including the genera Vitis and Parthenocissus. However, the latter has not previously been reported as a host in Germany. Here, it is shown on the basis of phylogenetic and morphological data that recent outbreaks of downy mildew on Parthenocissus tricuspidata in the Stuttgart area of Southern Germany are not caused by Plasmopara viticola, but by an undescribed species introduced here as Plasmopara muralis. The new species has rapidly spread in the Stuttgart area throughout the past 2 years and poses a potential threat to ornamental vines grown as wall cover should its spread not be controlled.


Downy mildews Molecular phylogeny Morphology Peronosporaceae Taxonomy 



I am grateful for the support of Ndambi Beninweck for help with measurements, and of Fabian Runge and Christoph Rost, who helped in various aspects of this study. The present study was financially supported by the research funding programme “LOEWE – Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz" of the Ministry of Higher Education, Research, and the Arts of Hesse and by a grant from the German Science Foundation (DFG).


  1. Choi Y-J, Hong S-B, Shin H-D (2007a) Re-consideration of Peronospora farinosa infecting Spinacia oleracea as distinct species, Peronospora effusa. Mycol Res 111:381–391PubMedCrossRefGoogle Scholar
  2. Choi Y-J, Constantinescu O, Shin HD (2007b) A new downy mildew of the Rosaceae: Peronospora oblatispora sp. nov. (Chromista, Peronosporales). Nova Hedwig 85:93–101CrossRefGoogle Scholar
  3. Choi Y-J, Shin H-D, Thines M (2009a) Two novel Peronospora species are associated with recent reports of downy mildew on sages. Mycol Res 113:1340–1350PubMedCrossRefGoogle Scholar
  4. Choi Y-J, Kiss L, Vajna L, Shin H-D (2009b) Characterization of a Plasmopara species on Ambrosia artemisiifolia, and notes on P. halstedii, based on morphology and multiple gene phylogenies. Mycol Res 113:1127–1136PubMedCrossRefGoogle Scholar
  5. Cunnington JH (2006) DNA sequence variation supports multiple host-specialised taxa in the Peronospora viciae complex (Chromista: Peronosporales). Nova Hedwig 82:23–29CrossRefGoogle Scholar
  6. Ellett CW (1970) An annotated list of the Peronosporales of Ohio. Ohio J Sci 70:218–226Google Scholar
  7. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376PubMedCrossRefGoogle Scholar
  8. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  9. García-Blázquez G, Göker M, Voglmayr H, Martín MP, Tellería MT, Oberwinkler F (2008) Phylogeny of Peronospora, parasitic on Fabaceae, based on ITS sequences. Mycol Res 112:502–512PubMedCrossRefGoogle Scholar
  10. Gäumann E (1923) Beiträge zu einer Monographie der Gattung Peronospora Corda. Beitr Kryptogamenflora Schweiz 5:1–360Google Scholar
  11. Göker M, Riethmüller A, Voglmayr H, Weiß M, Oberwinkler F (2004) Phylogeny of Hyaloperonospora based on nuclear ribosomal internal transcribed spacer sequences. Mycol Prog 3:83–94CrossRefGoogle Scholar
  12. Göker M, Voglmayr H, García-Blázquez G, Oberwinkler F (2009) Species delimitation in downy mildews: the case of Hyaloperonospora in the light of nuclear ribosomal ITS and LSU sequences. Mycol Res 113:308–325PubMedCrossRefGoogle Scholar
  13. Golovina NP (1955) Sravnitelnaia karakteristika obraztov Plasmopara viticola Berl. et de Toni iz raznîh stran. Botaniceskie materiali otdela sporovîh rastenii, Botaniceskovo institute im. V.L. Komarova, izd. A.N. SSSR 10:138–144Google Scholar
  14. Halsted BD (1890) Notes upon Peronosporeae for 1890. Bot Gaz 15:320–324CrossRefGoogle Scholar
  15. Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755PubMedCrossRefGoogle Scholar
  16. Katoh K, Toh H (2008) Improved accuracy of multiple ncRNA alignment by incorporating structural information into a MAFFT-based framework. BMC Bioinform 9:212CrossRefGoogle Scholar
  17. Katoh K, Misawa M, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059–3066PubMedCrossRefGoogle Scholar
  18. Komjáti H, Walcz I, Virányi F, Zipper R, Thines M, Spring O (2007) Characteristics of a Plasmopara angustiterminalis isolate from Xanthium strumarium. Eur J Plant Pathol 119:421–428CrossRefGoogle Scholar
  19. Landa BB, Montes-Borrego M, Muñoz-Ledesma FJ, Jiménez-Díaz RM (2007) Phylogenetic analysis of downy mildew pathogens of opium poppy and PCR-based in planta and seed detection of Peronospora arborescens. Phytopathology 97:1380–1390PubMedCrossRefGoogle Scholar
  20. Mosher L (1857) The mildew and rot in the grape. Cincinnatus 2:498–503Google Scholar
  21. Protsenko A (1946) Novîi vozbuditel mildiu na Amurskom vinograde. Vinod Vinograd SSSR 7:30–32Google Scholar
  22. Rafailă C, Sevcenco V, David Z (1968) Contributions to the biology of Plasmopara viticola. J Phytopathol 63:328–336CrossRefGoogle Scholar
  23. Riethmüller A, Voglmayr H, Göker M, Weiß M, Oberwinkler F (2002) Phylogenetic relationships of the downy mildews (Peronosporales) and related groups based on nuclear large subunit ribosomal DNA sequences. Mycologia 94:834–849PubMedCrossRefGoogle Scholar
  24. Săvulescu T (1941) Mana vitei de vie Studiu monografie. Academia română, Studii şi cercetări, LII, Bucharest, RomaniaGoogle Scholar
  25. Săvulescu T, Săvulescu O (1952) Studiul morphologic, biologic şi sistematic al genurilor Sclerospora, Basidiophora, Plasmopara şi Peronoplasmopara. Edit. Acad. RPR, Bucharest, RomaniaGoogle Scholar
  26. Spring O, Voglmayr H, Riethmüller A, Oberwinkler F (2003) Characterization of a Plasmopara isolate from Helianthus x laetiflorus based on cross infection, morphological, fatty acids and molecular phylogenetic data. Mycol Prog 2:163–170CrossRefGoogle Scholar
  27. Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690PubMedCrossRefGoogle Scholar
  28. Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web-servers. Syst Biol 57:758–771PubMedCrossRefGoogle Scholar
  29. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599PubMedCrossRefGoogle Scholar
  30. Telle S, Thines M (2008) Amplification of cox2 ( 620 bp) from 2 mg of up to 129 years old herbarium specimens, comparing 19 extraction methods and 15 polymerases. PLoS ONE 3:e3584PubMedCrossRefGoogle Scholar
  31. Thines M (2007) Characterisation and phylogeny of repeated elements giving rise to exceptional length of ITS2 in several downy mildew genera (Peronosporaceae). Fungal Genet Biol 44:199–207PubMedCrossRefGoogle Scholar
  32. Thines M, Telle S, Ploch S, Runge F (2009) Identity of the downy mildew pathogens of basil, coleus, and sage with implications for quarantine measures. Mycol Res 113:532–540PubMedCrossRefGoogle Scholar
  33. Thines M, Runge F, Telle S, Voglmayr H (2010) Phylogenetic investigations in the downy mildew genus Bremia reveal several distinct lineages and a species with a presumably exceptional wide host range. Eur J Plant Pathol (in press)Google Scholar
  34. Viennot-Bourgin G (1949) Les champignons parasites des plantes cultivées. Masson & Cie, ParisGoogle Scholar
  35. Viennot-Bourgin (1981) History and importance of downy mildews. In: Spencer DM (ed) The downy mildews. Academic, London, pp 1–15Google Scholar
  36. Voglmayr H (2003) Phylogenetic relationships of Peronospora and related genera based on nuclear ribosomal ITS sequences. Mycol Res 107:1132–1142PubMedCrossRefGoogle Scholar
  37. Voglmayr H, Thines M (2007) Phylogenetic relationships and nomenclature of Bremiella sphaerosperma (Chromista, Peronosporales). Mycotaxon 100:11–20Google Scholar
  38. Voglmayr H, Constantinescu O (2008) Revision and reclassification of three Plasmopara species based on morphological and molecular phylogenetic data. Mycol Res 112:487–501PubMedCrossRefGoogle Scholar
  39. Voglmayr H, Riethmüller A, Göker M, Weiss M, Oberwinkler F (2004) Phylogenetic relationships of Plasmopara, Bremia and other genera of downy mildew pathogens with pyriform haustoria based on Bayesian analysis of partial LSU rDNA sequence data. Mycol Res 108:1011–1024PubMedCrossRefGoogle Scholar
  40. Voglmayr H, Fatehi J, Constantinescu O (2006) Revision of Plasmopara (Chromista, Peronosporales) parasitic on Geraniaceae. Mycol Res 110:633–645PubMedCrossRefGoogle Scholar
  41. Wilson GW (1908) Studies in North American Peronosporales - IV.Host index. Bull Torrey Bot Club 35:543–554CrossRefGoogle Scholar
  42. Yerkes WD, Shaw CG (1959) Taxonomy of Peronospora species on Cruciferae and Chenopodiaceae. Phytopathology 49:499–507Google Scholar

Copyright information

© German Mycological Society and Springer 2010

Authors and Affiliations

  1. 1.Biodiversity and Climate Research Centre (BiK-F)Frankfurt (Main)Germany
  2. 2.Department of Biological Sciences, Institute of Ecology, Evolution and DiversityJohann Wolfgang Goethe UniversityFrankfurt (Main)Germany

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