Phlebopus portentosus is a well-known edible wild mushroom in the tropical part of Yunnan province of China. The mushrooms grow around natural or planted trees of Delonix regia, Mangifera indica, Coffea arabica, Citrus grandis, Artocarpus heterophyllus and Quercus spp. The technology of cultivation of P. portentosus was developed and its biotrophic status examined at Xishuangbanna, Yunnan, China. Uncultivated red soils with and without host plants of C. arabica, C. grandis and M. indica were inoculated with solid inocula of P. portentosus. Matured mushrooms were produced from both inoculated soils, with and without the host plants, 20–30 days after inoculation. No mycorrhizal structures were detected although the fungal mycelia colonized the plant root surfaces. Results show that P. portentosus is a saprobic rather than a symbiotic fungus. Based on this discovery, two methods of cultivation of P. portentosus were developed. Non-sterilized agricultural soils in polypropylene bottles or bags were inoculated with the fungal solid inocula and incubated at a mushroom house. Fruiting-body primordia were produced from the inoculated soils 20–30 days after inoculation. Soil-cased sawdust logs inoculated with P. portentosus produced primordia 10–15 days after casing only. The primordia developed into mature mushrooms 5–6 days later with weights ranging from 20.0 to 135.0 g. The identity of the cultivated fruiting bodies was confirmed by morphological and molecular methods. Our molecular phylogeny based on the Internal Transcribed Spacer sequences from our cultivated isolate and Genbank accessions provides preliminary insight into the phylogeogrpahy of P. portentosus.
Edible fungus Saprobic black bolete Cultivation Yunnan