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Mycological Progress

, Volume 10, Issue 2, pp 149–155 | Cite as

Molecular phylogeny of Psilocybe cyanescens complex in Europe, with reference to the position of the secotioid Weraroa novae-zelandiae

  • Jan Borovička
  • Machiel E. Noordeloos
  • Milan Gryndler
  • Miroslav OborníkEmail author
Original article

Abstract

A phylogenetic analysis with three molecular markers was undertaken to test the hypothesis that the complex of Psilocybe cyanescens in Europe consists of several, morphologically distinct species. The results support the existence of two molecularly well-supported morphological groups, that of Psilocybe cyanescens and P. azurescens on the one hand, and the complex of P. serbica on the other. However, in the last group, no sequence variability within the three molecular markers from P. serbica and related taxa P. bohemica, P. arcana, and P. moravica was found. It was decided, therefore, to merge these taxa into P. serbica, and to distinguish them below species level. It was also demonstrated that the secotioid Weraroa novae-zelandiae belongs to the P. cyanescens species complex. Accordingly, it was transferred to Psilocybe as P. weraroa, nomen novum.

Keywords

Strophariaceae Psilocybe serbica Psilocybe cyanescens Weraroa novae-zelandiae DNA markers Phylogeny 

Notes

Acknowledgements

We are very grateful to Michal Tomšovský for valuable advice and to our colleagues who kindly provided their collections for study: anonymous donators, Jürgen Hechler, Egon Horak, Zdenko Tkalčec, and Ruben Walleyn (†). The kind assistance of curators Lenka Edrová and Jan Holec (herbarium PRM; Mycological Department, National Museum, Prague) is also greatly appreciated. This research was supported by Institutional Research Plans (IRP) AV0Z30130516 (Institute of Geology, ASCR, Prague), AV0Z10480505 (Nuclear Physics Institute, ASCR, Řež near Prague), IRP AV0Z50200510 (Institute of Microbiology, ASCR, Prague), research project of the Institute of Parasitology ASCR (Z60220518) and Ministry of Education of the Czech Republic (6007665801). An indirect support was obtained from the project IAA600480801 (The Grant Agency of the Academy of Sciences of the Czech Republic).

Supplementary material

11557_2010_684_MOESM1_ESM.xls (22 kb)
Suplementary Table S1 Sequences used in this study and downloaded from GeneBank™. (XLS 22 kb)
11557_2010_684_MOESM2_ESM.xls (28 kb)
Suplementary Table S2 Morphological, phenological and ecological characters used to construct the morphological matrix. (XLS 28 kb)

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Copyright information

© German Mycological Society and Springer 2010

Authors and Affiliations

  • Jan Borovička
    • 1
    • 2
  • Machiel E. Noordeloos
    • 3
  • Milan Gryndler
    • 4
  • Miroslav Oborník
    • 5
    Email author
  1. 1.Institute of GeologyAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  2. 2.Nuclear Physics InstituteAcademy of Sciences of the Czech RepublicPragueCzech Republic
  3. 3.Netherlands Centre for Biodiversity Naturalis (section NHN)Leiden UniversityLeidenThe Netherlands
  4. 4.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague 4Czech Republic
  5. 5.Biology Centre, Institute of ParasitologyAcademy of Sciences of the Czech RepublicČeské BudějoviceCzech Republic

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