Mycological Progress

, Volume 10, Issue 1, pp 93–99 | Cite as

Tricholomopsis flammula (Basidiomycota, Agaricales)—molecular taxonomy, delimitation, variability and ecology

  • Jan HolecEmail author
  • Miroslav Kolařík
Original article


Tricholomopsis flammula, recently validated, was studied by molecular methods to clarify its taxonomic position. Sequences of T. flammulla form a distinct clade sister to a well-supported clade of T. rutilans sequences, whereas T. decora forms a cluster standing apart from them. T. flammula really is a good species separate from T. rutilans and T. decora, well distinguishable both phenotypically and molecularly. The infraspecific variability revealed by molecular methods is expressed in slight differences among some of the collections studied. The brief characteristics, selected descriptions and illustrations, key diagnostic characters and data on habitats and substrates of T. flammula are given. A discussion on variability of its basidiocarps is added.


Tricholomopsis Europe Nuclear rDNA Sequencing Description Habitats 



We thank A. Hausknecht (Austria) and J. Borovička (Czech Republic) for information on their collections of T. flammula and two anonymous reviewers for their valuable comments. The work by J. Holec was financially supported by the Ministry of Culture of the Czech Republic (project MK00002327201) and by the internal grant project of the National Museum, Prague. M. Kolařík was sponsored by the grants GA ČR 206/08/P322 and MSM 6007665801.


  1. Boekhout T, Noordelooos ME (1999) Tricholomopsis. In: Bas C, Kuyper TW, Noordeloos ME, Vellinga EC (eds) Flora agaricina neerlandica, vol. 4. Balkema, Rotterdam, pp 151–152Google Scholar
  2. Breitenbach J, Kränzlin F (1991) Pilze der Schweiz. Band 3. Röhrlinge und Blätterpilze 1. Mykologia, LuzernGoogle Scholar
  3. Courtecuisse R (2008) 5.—Nouvelles combinaisons et nouveaux noms nécessaires suite à la mise au point du référentiel des noms de champignons présents sur le territoire national métropolitain (1—Basidiomycètes). Doc Mycol 34(135–136):48–52Google Scholar
  4. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797PubMedCrossRefGoogle Scholar
  5. Gardes M, Bruns D (1993) ITS primers with enhanced specificity for basidiomycetes: application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118PubMedCrossRefGoogle Scholar
  6. Guindon S, Lethiec F, Duroux P, Gascuel O (2005) PHYML Online—a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33:557–559CrossRefGoogle Scholar
  7. Holec J (2009) Valid publication of the name Tricholomopsis flammula (Fungi, Basidiomycota, Tricholomataceae), a species clearly separated from T. rutilans. J National Mus (Prague), Nat Hist Ser 178(3):7–13Google Scholar
  8. Hulcr J, Kolařík M, Kirkendal LR (2007) A new record of fungus-beetle symbiosis in Scolytodes bark beetles (Scolytinae, Curculionidae, Coleoptera). Symbiosis 43:151–159Google Scholar
  9. Johnston A (2006) Capilano river regional park fungi, Tricholomopsis rutilans. Accessed 27 April 2010
  10. Kirk PM, Cannon PF, Minter DW, Stalpers JA (eds) (2008) Dictionary of the fungi, 10th edn. CAB International, WallingfordGoogle Scholar
  11. Kornerup A, Wanscher JH (1981) Taschenlexikon der Farben, 3rd edn. Muster-Schmidt, ZürichGoogle Scholar
  12. Krieglsteiner GJ, Enderle M (1986) Über neue, seltene, kritische Makromyzeten in der Bundesrepublik Deutschland (Mitteleuropa) VII. Beitr Kenntn Pilze Mitteleuropas 2:125–162Google Scholar
  13. Krisai-Greilhuber I, Voglmayr H (2000) Tricholomopsis flammula of Upper Austria. Beitr Naturkd Oberösterreichs 9:701–704Google Scholar
  14. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163PubMedCrossRefGoogle Scholar
  15. Ludwig E (2001) Pilzkompendium, Band 1, Beschreibungen. IHW, EchingGoogle Scholar
  16. Makela K (2006) Capilano river regional park fungi, Tricholomopsis decora. Accessed 27 April 2010
  17. Matheny PB, Curtis JM, Hofstetter V, Aime MC, Moncalvo JM, Ge ZW, Yang ZL, Slot JC, Ammirati JF, Baroni TJ, Bougher NL, Hughes KW, Lodge DJ, Kerrigan RW, Seidl MT, Aanen DK, DeNitis M, Daniele GM, Desjardin DE, Kropp BR, Norvell LL, Parker A, Vellinga EC, Vilgalys R, Hibbett DS (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98:982–995PubMedCrossRefGoogle Scholar
  18. Métrod G (1946) Champignons du Jura. Rev Mycol 11:74–81Google Scholar
  19. Moncalvo JM, Vilgalys R, Redhead SA, Johnson JE, James TY, Aime MC, Hofstetter V, Verduin SJW, Larsson E, Baroni TJ, Thorn RG, Jacobsson S, Clémençon H, Miller OK Jr (2002) One hundred and seventeen clades of euagarics. Mol Phyl Evol 23:357–400CrossRefGoogle Scholar
  20. Moser M, Jülich W (1993) Farbatlas der Basidiomyceten, vol 11. Gustav Fischer, BerlinGoogle Scholar
  21. O'Donnell K (1993) Fusarium and its near relatives. In: Reynolds DR, Taylor JW (eds) The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. CAB International, Wallingford, pp 225–233Google Scholar
  22. Petersen RH, Hughes KW (2010) TENN—University of Tennessee herbarium, Fungal Herbarium, Tricholomopsis. Accessed 27 April 2010
  23. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574PubMedCrossRefGoogle Scholar
  24. Shanks K, Vilgalys R (2000) Tricholomopsis rutilans, AF319423. Accessed 27 April 2010
  25. Smith AH (1960) Tricholomopsis (Agaricales) in the Western Hemisphere. Brittonia 12:41–70CrossRefGoogle Scholar
  26. Thiers B (2010) Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden's Virtual Herbarium. Accessed 16 March 2010
  27. Vauras J (2009) Tricholomopsis osiliensis, a new agaric species from Estonia. Folia Cryptogam Est 45:87–89Google Scholar
  28. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246PubMedGoogle Scholar

Copyright information

© German Mycological Society and Springer 2010

Authors and Affiliations

  1. 1.Mycological DepartmentNational MuseumPraha 1Czech Republic
  2. 2.Institute of Microbiology ASCRPraha 4Czech Republic
  3. 3.Department of Botany, Faculty of ScienceCharles UniversityPraha 2Czech Republic

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