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Mycological Progress

, Volume 9, Issue 4, pp 567–574 | Cite as

Micronematobotrys, a new genus and its phylogenetic placement based on rDNA sequence analyses

  • Xiang Sun
  • Liang-Dong Guo
Original article

Abstract

A new hyphomycetous fungus, Micronematobotrys verrucosus, isolated as an endophyte from Quercus liaotungensis and Ulmus macrocarpa in the Dongling Mountain of Beijing is described as a new monotypic genus represented by M. verrucosus. This new species differs morphologically from Botrytis-like fungi and other similar genera in possessing micronematous conidiophores and colored verrucose conidia. The phylogenetic relationships of Micronematobotrys among sexual ascomycetes are examined based on 18S and 28S rDNA sequence data using maximum parsimony and Bayesian methods. The phylogenetic analyses suggest that M. verrucosus is a member of Pyronemataceae in Pezizales.

Keywords

Micronematobotrys verrucosus Endophyte Phylogenetic analyses Pyronemataceae 

Notes

Acknowledgements

We would thank Prof. Jian-Yun Zhuang of Institute of Microbiology, Chinese Academy of Sciences for revision of Latin descriptions and valuable comments, Prof. Kevin D. Hyde of Mae Fah Luang University for English correction and valuable comments, and anonymous reviewers for suggestions of improvements of the manuscript. This work was supported by the National Natural Science Foundation of China Grants (Nos. 30930005 and 30870087) and by the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant Nos. KSCX2-YW-G-068 and KSCX2-YW-Z-0935).

References

  1. Carmichael JW (1980) Genera of hyphomycetes. The University of Alberta Press, EdmontonGoogle Scholar
  2. Dissing H (1985) Smardaea purpurea (Pezizales), another new species from Graubünden, Switzerland. Sydowia 38:35–40Google Scholar
  3. Egger KN (1984) Pyropyxis, a new pyrophilous operculate discomycete with a Dichobotrys anamorph. Can J Bot 62:705–708. doi: 10.1139/b84-103 CrossRefGoogle Scholar
  4. Ellis MB (1971) Dematiaceous Hyphomycetes. CAB International, WallingfordGoogle Scholar
  5. Guo LD, Hyde KD, Liew ECY (2000) Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. New Phytol 147:617–630. doi: 10.1111/j.1469-8137.2000.00716 CrossRefGoogle Scholar
  6. Guo LD, Huang GR, Wang Y, He WH, Zheng WH, Hyde KD (2003) Molecular identification of white morphotype strains of endophytic fungi from Pinus tabulaeformis. Mycol Res 107:680–688. doi: 10.1017/S0953756203007834 CrossRefPubMedGoogle Scholar
  7. Hennebert GL (1973) Botrytis and Botrytis-like genera. Persoonia 7:183–204Google Scholar
  8. Huang WY, Cai YZ, Hyde KD, Corke H, Sun M (2008) Biodiversity of endophytic fungi associated with 29 traditional Chinese medicinal plants. Fungal Divers 33:61–75Google Scholar
  9. Huang WY, Cai YZ, Surveswaran S, Hyde KD, Corke H, Sun M (2009) Molecular phylogenetic identification of endophytic fungi isolated from three Artemisia species. Fungal Divers 36:69–88Google Scholar
  10. Huelsenbeck JP, Ronquist F (2001) MRBAYES: bayesian inference of phylogeny trees. Bioinformatics 17:754–755CrossRefPubMedGoogle Scholar
  11. Hyde KD, Soytong K (2008) The fungal endophyte dilemma. Fungal Divers 33:163–173Google Scholar
  12. Korf RP, Zhuang WY (1991) A preliminary discomycete flora of Macronesia: part 15, Terfeziaceae, and Otideaceae, Otideoideae. Mycotaxon 40:413–433Google Scholar
  13. Marek SM, Hansen K, Romanish M, Thorn RG (2009) Molecular systematics of the cotton root rot pathogen, Phymatotrichopsis omnivora. Persoonia 22:63–74PubMedGoogle Scholar
  14. Mitchell AM, Strobel GA, Hess WM, Vargas PN, Ezra D (2008) Muscodor crispans, a novel endophyte from Ananas ananassoides in the Bolivian Amazon. Fungal Divers 31:37–43Google Scholar
  15. Mouton PA (1953) Phymatotrichum omnivorum (Shear) dug., pourridié du cotonnier. Rev Mycol 18(suppl. 2):69–87Google Scholar
  16. Nugent KG, Saville BJ (2004) Forensic analysis of hallucinogenic fungi: a DNA-based approach. Forensic Sci Int 140:147–157. doi: 10.1016/j.forsciint.2003.11.022 CrossRefPubMedGoogle Scholar
  17. Nylander JAA (2008) MrModeltest 2.3 README. http://www.abc.se/~nylander/mrmodeltest2/mrmodeltest2.html. Accessed 22 May 2008
  18. Paden JW (1984) A new genus of hyphomycetes with teleomorphs in the sarcoscyphaceae (pezizales, sarcoscyphineae). Can J Bot 62:211–218. doi: 10.1139/b84-035 CrossRefGoogle Scholar
  19. Papendorf MC, Upadhyay HP (1969) Botryoderma lateritium and B. rostratum gen. et spp. nov. from soil in South Africa and Brazil. Trans Br Mycol Soc 52:257–265CrossRefGoogle Scholar
  20. Perry BA, Hansen K, Pfister DH (2007) A phylogenetic overview of the family pyronemataceae (ascomycota, pezizales). Mycol Res 111:549–571. doi: 10.1016/j.mycres.2007.03.014 CrossRefPubMedGoogle Scholar
  21. Saccardo PA (1889) Sylloge fungorum omnium hucusque cognitorum, vol 8. Pavia, ItalyGoogle Scholar
  22. Sun X, Guo LD (2007) Endophytic fungi - VI. Ciliophora quercus sp. nov. from China. Nova Hedwigia 85:403–406. doi: 10.1127/0029-5035/2007/0085-0403 CrossRefGoogle Scholar
  23. Sun JQ, Guo LD, Zang W, Ping WX, Chi DF (2008) Diversity and ecological distribution of endophytic fungi associated with medicinal plants. Sci China Ser C 51:751–759. doi: 10.1007/s11427-008-0091-z CrossRefGoogle Scholar
  24. Svrček M (1969) Nové rody operkulátních diskomycetů (Pezizales). Česká Mykol 23:83–96Google Scholar
  25. Swofford DL (2002) Phylogenetic Analysis Using Parsimony (and other methods). Version 4, Sinauer, Sunderland, Mass. http://paup.csit.fsu.edu/Cmd_ref_v2.pdf
  26. Tejesvi MV, Tamhankar SA, Kini KR, Rao VS, Prakash HS (2009) Phylogenetic analysis of endophytic Pestalotiopsis species from ethnopharmaceutically important medicinal trees. Fungal Divers 38:167–183Google Scholar
  27. Thompson J, Gibson T, Plewniak F, Jeanmougin F, Higgins D (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefPubMedGoogle Scholar
  28. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several cryptococcus species. J Bacteriol 172:4238–4246PubMedGoogle Scholar
  29. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogeneics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, San Diego, pp 315–322Google Scholar

Copyright information

© German Mycological Society and Springer 2010

Authors and Affiliations

  1. 1.Key Laboratory of Systematic Mycology & Lichenology, Institute of MicrobiologyChinese Academy of SciencesBeijingChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina

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