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Mycological Progress

, Volume 9, Issue 2, pp 267–280 | Cite as

Four ectomycorrhizae of Pyronemataceae (Pezizomycetes) on Chinese Pine (Pinus tabulaeformis): morpho-anatomical and molecular-phylogenetic analyses

  • Jie Wei
  • Derek Peršoh
  • Reinhard Agerer
Original Article

Abstract

Morphological and anatomical characters of four ectomycorrhizae with affinities to the genera Humaria, Geopora, and Trichophaea of Pyronemataceae (Pezizomycetes, Ascomycota) on Chinese Pine (Pinus tabulaeformis) are described. The ectomycorrhizae are yellowish brown to brown, and have pseudoparenchymatous outer mantle layers and partially warty emanating hyphae with thick walls and without clamps. Intrahyphal hyphae are present, and no rhizomorphs are formed. The four ectomycorrhizae are distinguishable by differences in cell shape of outer mantle layers and the presence of cystidia. Ectomycorrhizae of a possible Humaria species (Pinirhiza humarioides) lack cystidia and have irregularly inflated cells on the outer mantle layer that are connected with thin septa. The two ectomycorrhizae showing probable affinities to Geopora species (“P. daqingensis” and “P. geoporoides”) possess row-like arranged cells in the outer mantle layer and cell heaps, and differ by the presence or absence of cystidia as well as by the structure of the inner mantle layers. Ectomycorrhizae likely having been formed by a Trichophaea species (“P. trichophaeoides”) have oval to polygonal cells and no cystidia. The possible taxa affiliations were assessed by molecular-phylogenetic analyses of the internal transcribed spacer (ITS) and partial large subunit (LSU) nrDNA. Morphological and anatomical characters are discussed against the background of the LSU phylogeny.

Keywords

Anatomy Ectomycorrhiza Morphology Molecular-phylogenetic analyses Pyronemataceae 

Notes

Acknowledgements

This study was financially supported by German Academic Exchange Service (DAAD). We thank Prof. Yan Wei (Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, China) for his support of field work and for providing equipment, Fan Yongjun (Inner Mongolia Agricultural University) for assistance in the field, and the Managment Authority of Helan Mountain and Heilihe Natural Reserve for sampling permission. We thank two anonymous reviewers, whose suggestions significantly improved the manuscript.

Supplementary material

11557_2009_637_Fig1_ESM.jpg (440 kb)
Fig. 17

Molecular-phylogenetic relationships among selected Pyronemataceae. Best scoring tree found by the RAxML analysis of LSU nrDNA sequences. Bootstrap support values above 50% are noted above or to the left of the respective branches. GenBank accession numbers are given in parentheses following the species names (JPEG 440 kb)

11557_2009_637_Fig1_ESM.tif (334 kb)
High Resolution Image (TIFF 333 kb)
11557_2009_637_Fig2_ESM.jpg (498 kb)
Fig. 18

Molecular-phylogenetic relationships among selected Pyronemataceae 50% Majority Rule Consensus Tree (2057 steps, CI = 0.245, RI = 0.773) of the 3341 most parsimonious trees (2050 steps) found by Parsimony Ratchet. Posterior Probabilities are noted above or to the left of the respective branches GenBank accession numbers are given in parentheses following the species names (JPEG 497 kb)

11557_2009_637_Fig2_ESM.tif (360 kb)
High Resolution Image (TIFF 359 kb)

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Copyright information

© German Mycological Society and Springer 2009

Authors and Affiliations

  1. 1.Department Biology I, Division of Organismic Biology: MycologyUniversity of MunichMunichGermany

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