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Mycological Progress

, Volume 2, Issue 3, pp 219–225 | Cite as

The role of nitrate reductase in the degradation of the explosive RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) by Penicillium sp. AK96151

  • Heidrun Anke
  • Andrea Kuhn
  • Roland W. S. Weber
Article

Abstract

In liquid culture on a defined growth medium, Penicillium sp. AK96151 efficiently degraded the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX, hexogen), causing > 80 % disappearance after 10 d. RDX degradation was reduced to a basal level (< 15 % degraded after 10 d) by the presence of > 150 μM ammonium ions or when the molybdenum component of the medium was replaced by sodium tungstate. An equivalent effect of ammonium, molybdenum and tungsten was observed in protoplasts of this fungus assayed for nitrate reductase activity. This enzyme was not inhibited by RDX itself. The involvement of a nitrate reductase in RDX degradation by Penicillium has practical implications for bioremediation strategies which are discussed.

Keywords

Explosive Molybdenum Penicillium Nitrate Reductase Triazine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© DGfM 2003

Authors and Affiliations

  • Heidrun Anke
    • 1
  • Andrea Kuhn
    • 1
  • Roland W. S. Weber
    • 2
  1. 1.Institute of Biotechnology and Drug Research (IBWF)KaiserslauternGermany
  2. 2.Department of BiotechnologyUniversity of KaiserslauternKaiserslauternGermany

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