Advertisement

Potato Research

, Volume 61, Issue 4, pp 297–308 | Cite as

Pathogenicity of Three Different Entomopathogenic Fungi, Metarhizium anisopliae IRAN 2252, Nomuraea rileyi IRAN 1020C and Paecilomyces tenuipes IRAN 1026C Against the Potato Tuber Moth, Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae)

  • F. Khorrami
  • F. MehrkhouEmail author
  • M. Mahmoudian
  • Y. Ghosta
Article

Abstract

The potato tuber moth (PTM), Phthorimaea operculella Zeller, is a prominent pest of potato in many potato cultivation areas such as Iran. Since the potato is one of the most important sources of human food, non-chemical control of the pest is essential. Application of entomopathogenic fungi is a low-risk alternative method to synthetic chemical insecticides. Therefore, we examined the efficacy of Metarhizium anisopliae IRAN 2252, Nomuraea rileyi IRAN 1020C and Paecilomyces tenuipes IRAN 1026C against neonate larvae and eggs of P. operculella under laboratory (26 ± 1 °C, 60 ± 5% relative humidity (RH) and a photoperiod of 16-h light: 8-h dark (L:D)) and greenhouse (26 ± 2 °C, 55 ± 5% RH and a photoperiod of 14-h L: 10-h D) conditions. Probit analysis of toxicity showed that N. rileyi was the most effective fungus against neonate larval penetration into potato tuber with a lethal concentration resulting in 50% mortality (LC50) equivalent to 1 × 103 conidia/ml. Moreover, adult emergence and first generation progenies of PTM were reduced when the LC25 value of each fungus was used. N. rileyi showed the highest toxicity against neonate larvae, pupae and adult emergence of PTM under laboratory and greenhouse conditions with LC50 values equivalent to 2.6 × 102 and 4.2 × 103 conidia/ml, respectively. N. rileyi caused significant egg hatching reduction (LC50 = 4.8 × 104). The results indicated that these entomopathogenic fungi had ovicidal and larvicidal activities against P. operculella and can be used in integrated pest management (IPM) programmes.

Keywords

Entomopathogenic fungi Potato Potato tuber moth Virulence 

Notes

Funding

This study was funded by Urmia University, Urmia, Iran (grant number: D 10/485).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

References

  1. Collantes LG, Raman K, Cisneros FH (1986) Effect of six synthetic pyrethroids on two populations of potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), in Peru. Crop Prot 5:355–357CrossRefGoogle Scholar
  2. Das GP (1995) Plants used in controlling the potato tuber moth, Phthorimaea operculella (Zeller). Crop Prot 14:631–636CrossRefGoogle Scholar
  3. Hall RA (1981) The fungus Verticillium lecanii as a microbial insecticide against aphids and scales. In: Burges HD (ed) Microbial Control of Pests and Plant Diseases. Academic Press, London, New YorkGoogle Scholar
  4. Ignoffo CM (1981) The fungus, Nomuraea rileyi as a microbial insecticide. in: Microbial control of pests and plant diseases, 1970–80 H.D. Burges, ed. Academic Press, London, pp 513–538Google Scholar
  5. Jensen A, Hamm P, Schreiber A, DeBano S (2005) Prepare for tuber moth in 2005. Potato Prog 5:1–4Google Scholar
  6. Kiuchi M, Yasui H, Hayasaka S, Kamimura M (2003) Entomogenous fungus Nomuraea rileyi inhibits host insect moulting by C22-oxidizing inactivation of hemolymph ecdysteroids. Arch Insect Biochem 52:35–44CrossRefGoogle Scholar
  7. Lacey AL, Kroschel J, Arthurs PS, Rosa FL (2010) Microbial control of the potato tuber moth Phthorimaea operculella (Lepidoptera: Gelechiidae). Rev Colomb Entomol 36(2):181–189Google Scholar
  8. Lacy AL, Neven GL (2006) The potential of the fungus, Muscodor albus, as a microbial control agent of potato tuber moth (Lepidoptera: Gelechiidae) in stored potatoes. J Invertebr Pathol 91:195–198CrossRefGoogle Scholar
  9. Lee M, Yoon CS, Yi J, Cho JR, Kim HS (2005) Cellular immune responses and FAD-glucose dehydrogenase activity of Mamestra brassicae (Lepidoptera: Noctuidae) challenged with three species of entomopathogenic fungi. Physiol Entomol 30:287–292CrossRefGoogle Scholar
  10. Llanderal-Cazares C, Lagunes-Tejada A, Carrillo-Sanchez JL, Sosa-Moss C, Vera-Graziano J, Bravo-ojica H (1996) Susceptibility of Phthorimaea operculella (Zeller) to insecticides. J Entomol Sci 31:420–426CrossRefGoogle Scholar
  11. Marannino P, Santiago-Alvarez C, de Lillo E, Quesada-Moraga E (2006) A new bioassay method reveals pathogenicity of Metarhizium anisopliae and Beauveria bassiana against early stages of Capnodis tenebrionis (Coleoptera; Buprestidae). J Invertebr Pathol 93:210–213CrossRefGoogle Scholar
  12. Neves PMOJ, Hirose E, Tchujo PT, Moino AJR (2001) Compatibility of entomopathogenic fungi with neonicotinoids insecticides. Neotrop Entomol 30(2):263–268CrossRefGoogle Scholar
  13. Patill RK, Bhagat YS, Halappa B, Bhat RS (2014) Evaluation of entomopathogenic fungus, Nomuraea rileyi (Farlow) samson for the control of groundnut Spodoptera litura (F.) and its compatibility with synthetic and botanical pesticides. J Biopest 7:106–115Google Scholar
  14. Sabbour MM, Abdel-Raheem M (2015) Efficacy of Beauveria brongniartii and Nomuraea rileyi against the potato tuber moth, Phthorimaea operculella (zeller). AJIRAS 1(6):197–202Google Scholar
  15. Sabbour M, Singer SM (2014) Evaluations of two isolated Paecilomyces against Phthorimaea operculella (Lepidoptera: Gelechiidae) under laboratory and field conditions. IJSR 3(9):1136–1139Google Scholar
  16. Santi L, Silva WOBD, Berger M, Guimaraes JA, Schrank A, Vainstein MH (2010) Conidial surface proteins of Metarhizium anisopliae: source of activities related with toxic effects, host penetration and pathogenesis. Toxicon 55:874–880CrossRefGoogle Scholar
  17. Sporleder M, Kroschel J, Quispe MRG, Lagnaoui A (2004) A temperature-based simulation model for the potato tuberworm, Phthorimaea operculella Zeller (Lepidoptera; Gelechiidae). Environ Entomol 33:477–486CrossRefGoogle Scholar
  18. Valencia-Jimenez A, Arboleda JW, Lopez Avila A, Grossi de Sa MF (2008) Digestive a-amylases from Tecia solanivora larvae (Lepidoptera: Gelechiidae): response to pH, temperature and plant amylase inhibitors. B Entomol Res 98:575–579CrossRefGoogle Scholar

Copyright information

© European Association for Potato Research 2018

Authors and Affiliations

  • F. Khorrami
    • 1
  • F. Mehrkhou
    • 1
    Email author
  • M. Mahmoudian
    • 2
  • Y. Ghosta
    • 1
  1. 1.Department of Plant Protection, Faculty of AgricultureUrmia UniversityUrmiaIran
  2. 2.Department of Nano Technology, Faculty of ScienceUrmia UniversityUrmiaIran

Personalised recommendations