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Potato Research

, Volume 59, Issue 1, pp 67–80 | Cite as

Fusarium spp. Causing Dry Rot on Potatoes in Norway and Development of a Real-Time PCR Method for Detection of Fusarium coeruleum

  • Pia HeltoftEmail author
  • May Bente Brurberg
  • Monica Skogen
  • Vinh Hong Le
  • Jafar Razzaghian
  • Arne Hermansen
Article

Abstract

The prevalence of Fusarium dry rot in potatoes produced in Norway was investigated in a survey for three consecutive years in the period 2010 to 2012. A total of 238 samples (comprising 23,800 tubers) were collected, representing different cultivars and production regions in Norway. Fusarium spp. were detected in 47% of the samples, with one to three species per sample. In total, 718 isolates of Fusarium spp. were recovered and identified to seven species. The most commonly isolated species was Fusarium coeruleum, comprising 59.6% of the total Fusarium isolates and found in 17.2% of the collected samples, followed by Fusarium avenaceum (27.2% of the isolates and found in 27.7% of the samples). Fusarium sambucinum was the third most prevalent species (6.4% in 8.8% of the samples) and Fusarium culmorum the fourth (5.2% in 6.3% of the samples). Less prevalent species included Fusarium cerealis, Fusarium graminearum, and Fusarium equiseti (<1% in 0.4 to 1.3% of the samples). F. coeruleum was the most prevalent species in northern and southwestern Norway, whereas F. avenaceum was dominating in eastern Norway. The potato cultivars Berber and Rutt were susceptible to all Fusarium spp. A new TaqMan real-time PCR assay specific for F. coeruleum was developed, which successfully identified Norwegian isolates. This and other previously developed real-time PCR assays targeting different Fusarium species were evaluated for their ability to detect latent infections in potatoes at harvest. This study provides new information on the current occurrence of different Fusarium species causing Fusarium dry rot in potatoes in Europe including areas far into the arctic in the north of Norway.

Keywords

Fusarium Molecular identification cultivars Potato dry rot Regions 

Notes

Acknowledgments

We would like to thank the agronomists and company representatives who supplied the samples investigated in this survey. We would also like to thank Ragnhild Hustveit, Julien Gomez, and Robert Nybråten for technical assistance. This research was funded by the Foundation for Research Levy on Agricultural Products and the Agricultural Agreement Research Fund, together with Norwegian food industry partners.

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Copyright information

© European Association for Potato Research 2016

Authors and Affiliations

  • Pia Heltoft
    • 1
    • 2
    Email author
  • May Bente Brurberg
    • 1
  • Monica Skogen
    • 1
  • Vinh Hong Le
    • 1
  • Jafar Razzaghian
    • 1
  • Arne Hermansen
    • 1
  1. 1.NIBIO, Norwegian Institute of Bioeconomy ResearchAasNorway
  2. 2.Department of Plant Sciences, Norwegian University of Life SciencesAasNorway

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