Skip to main content
Log in

Influence of Nitrogen Supply of Potato Plantlets on In Vitro Tuberization Pattern under Inductive and Non-inductive Conditions

  • Published:
Potato Research Aims and scope Submit manuscript

Abstract

The effects of nitrogen supply of in vitro plantlets on subsequent in vitro tuberization were examined in three potato varieties. In vitro plantlets were raised on MS media with varying amounts of ammonium nitrate, resulting in 70, 80, 100, 120 and 160% of the standard N content in MS medium and various nitrate : ammonium ratios. Tuberization was induced by adding an 8% sucrose solution to 4-week old plantlets and keeping them 2 weeks under short or long days (8 and 16 h illumination per day, respectively), followed by darkness for 9 weeks. Under long days, a lower nitrogen content in the medium and higher nitrate : ammonium ratio were favourable for the development of large tubers (>6 mm). Under short days, the total number of tubers increased with an increase in nitrogen content of the medium in cultivars Gülbaba and Désirée but not in cultivar Boró, while the number of large tubers was not significantly influenced by nitrogen supply. The tuber weight per plantlet was strongly influenced by genotype. Nitrogen supply of the in vitro plantlets had a prolonged effect on in vitro tuberization. Because the optimum nitrogen treatment is genotype specific, the quantity of large in vitro tubers could be increased by adjusting the nitrogen content of the medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Dobránszki J, Tábori KM, Ferenczy A (1998) Clearing up of the background of genotype-dependence in in vitro tuberization responses by multivariate analyses. Beiträge zur Züchtungsforschung-Bundesanstalt für Züchtungsforschung an Kulturpflanzen 4(2):37–39

    Google Scholar 

  • Evans NE (1993) A preliminary study on the effects of nitrogen supply on the growth-in vitro of 9 potato genotypes (Solanum spp). J Exp Bot 44:837–841

    Article  Google Scholar 

  • Garner N, Blake J (1989) The induction and development of potato microtubers in vitro on media free of growth regulating substances. Ann Bot 63:663–674

    CAS  Google Scholar 

  • Hussey G, Stacey NJ (1981) In vitro propagation of potato (Solanum tuberosum L.). Ann Bot 48:787–796

    Google Scholar 

  • Krauss A, Marshner H (1982) Influence of nitrogen nutrition, day length and temperature on contents of gibberellic and abscisic acid and on tuberization in potato plants. Potato Res 25:13–21

    Article  CAS  Google Scholar 

  • Lazányi J, Mándi M, Tóth IJ, Dobránszki J (1998) Potato breeding at the Research Centre of Debrecen Agricultural University. Beiträge zur Züchtungsforschung - Bundesanstalt für Züchtungsforschung an Kulturpflanzen 4(2):105–109

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Seabrook JE, Coleman AS, Levy D (1993) Effect of photoperiod on in vitro tuberization of potato (Solanum tuberosum L.). Plant Cell Tiss Organ Cult 34(1):43–51

    Article  Google Scholar 

  • Serio F, Elia A, Signore A, Santamaria P (2004) Influence of nitrogen form on yield and nitrate content of subirrigated early potato. J Sci Food Agric 84:1428–1432

    Article  CAS  Google Scholar 

  • Struik PC, Vreugdenhil D, Haverkort AJ, Bus CB, Dankert R (1991) Possible mechanisms of size hierarchy among tubers on one stem of potato (Solanum tuberosum L.) plant. Potato Res 34:187–203

    Article  Google Scholar 

  • Tábori K, Dobránszki J, Ferenczy A (1998) Some sprouting characteristics of microtuber. Abstract Book of Conference "Potato seed production by tissue culture". 25th-28th February 1998, Brussels, p. II/5.

  • Zarrabeitia A, Lejarcegui X, Veramendi J, Mingo-Castel AM (1997) Influence of nitrogen supply on micropropagation and subsequent microtuberization of four potato cultivars. Am Potato J 74:369–378

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Judit Dobránszki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dobránszki, J., Tábori, K.M. Influence of Nitrogen Supply of Potato Plantlets on In Vitro Tuberization Pattern under Inductive and Non-inductive Conditions. Potato Res. 53, 121–127 (2010). https://doi.org/10.1007/s11540-010-9156-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11540-010-9156-z

Keywords

Navigation