Central European Journal of Chemistry

, Volume 4, Issue 1, pp 194–206 | Cite as

AFM study of complement system assembly initiated by antigen-antibody complex

  • Almira Ramanaviciene
  • Valentinas Snitka
  • Rasa Mieliauskiene
  • Rolandas Kazlauskas
  • Arunas Ramanavicius
Article

Abstract

The shape and size of complement system C1 components assembled on a SiO2 surface after classical activation by antigen-antibody complex was determined by tapping mode atomic force microscopy (AFM). The SiO2 substrate was silanized and bovine leukemia virus proteins gp51 were covalently bound to the SiO2 substrate. Self-assembly of complement system proteins was investigated by AFM. Uniform coating of silanized surface by gp51 proteins was observed by AFM. After incubation of gp51 coated substrate in anti-gp51 antibody containing solution, Ag-Ab complexes were detected on the substrate surface by AFM. Then after treatment of Ag-Ab complex modified substrate by guinea-pig blood serum containing highly active complement system proteins for 3 minutes and 30 minutes features 2–3 times and 5–8 times higher in diameter and in height if compared with those observed after formation of Ag-Ab complex, were observed respectively on the surface of SiO2. This study revealed that AFM might be applied for the imaging of complement system assembly and provides valuable information that can be used to complement other well-established techniques.

Keywords

Bionanotechnology antigen-antibody docking complement system bovine leukemia virus AFM 

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Copyright information

© Central European Science Journals Warsaw and Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • Almira Ramanaviciene
    • 1
    • 2
  • Valentinas Snitka
    • 3
  • Rasa Mieliauskiene
    • 1
  • Rolandas Kazlauskas
    • 1
  • Arunas Ramanavicius
    • 1
    • 2
  1. 1.Department of Analytical and Environmental ChemistryVilnius UniversityVilnius 09Lithuania
  2. 2.Sector of Immunoanalysis and Informatics, Institute of ImmunologyVilnius UniversityVilnius 21Lithuania
  3. 3.Research Center for Microsystems and NanotechnologyKaunas University of TechnologyKaunasLithuania

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