Intervertebral disc degeneration: an experimental and numerical study using a rabbit model
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Animal models have been extensively used for the study of degenerative diseases and evaluation of new therapies to stop or even reverse the disease progression. The aim of this study is to reproduce lumbar intervertebral disc degeneration in a rabbit model by performing a percutaneous annular puncture at L4L5 level. The effect of this damage on the spine behaviour was analysed combining three different techniques: imaging processing, mechanical testing and computational modelling. Twenty New Zealand white rabbits were divided into control and experimental groups and followed up during 6 months. Intervertebral disc height, as well as nucleus area and signal intensity, decreased with degeneration while storage and loss moduli increased. Both changes may be related to the loss of water and tissue fibrosis. Similar but slighter changes were reported for adjacent discs. A finite element model was built based on MRI and mechanical testing findings to add new biomechanical information that cannot be obtained experimentally. Four stages were computationally simulated representing the different experimental phases. The numerical simulations showed that compressive stresses in the damaged and adjacent discs were modified with the progression of degeneration. Although extrapolation to humans should be carefully made, the use of numerical animal models combined with an experimental one could give a new insight of the overall mechanical behaviour of the spine.
KeywordsAnimal model Intervertebral disc degeneration Finite element model Experimental Lumbar spine
This work was supported by the Spanish Ministry of Economy and Competitiveness through projects DPI 2016-79302-R and by the Spanish Ministry of Education, Culture and Sports (Grant FPU13/01070).
Compliance with ethical standards
Conflict of interest
No conflict of interest should be declared by any of the authors.
This study was carried out in strict accordance with the recommendations in the Royal Decree 1201/2005 of 10 October 2005 (BOE from Oct. 21) on protection of animals used for experimentation and other scientific purposes. Experimental protocols were approved by the Committee on the Ethics of Animal Experiments of Minimally Invasive Surgery Centre Jesús Usón and by the Council of Agriculture and Rural Development of the Regional Government of Extremadura, Spain.
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