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Medical & Biological Engineering & Computing

, Volume 50, Issue 2, pp 117–126 | Cite as

Sensor-based cell and tissue screening for personalized cancer chemotherapy

  • Regina Kleinhans
  • Martin BrischweinEmail author
  • Pei Wang
  • Bernhard Becker
  • Franz Demmel
  • Tobias Schwarzenberger
  • Marlies Zottmann
  • Peter Wolf
  • Axel Niendorf
  • Bernhard WolfEmail author
Original Article

Abstract

Personalized tumor chemotherapy depends on reliable assay methods, either based on molecular “predictive biomarkers” or on a direct, functional ex vivo assessment of cellular chemosensitivity. As a member of the latter category, a novel high-content platform is described monitoring human mamma carcinoma explants in real time and label-free before, during and after an ex vivo modeled chemotherapy. Tissue explants are sliced with a vibratome and laid into the microreaction chambers of a 24-well sensor test plate. Within these ≈23 μl volume chambers, sensors for pH and dissolved oxygen record rates of cellular oxygen uptake and extracellular acidification. Robot-controlled fluid system and incubation are parts of the tissue culture maintenance system while an integrated microscope is used for process surveillance. Sliced surgical explants from breast cancerous tissue generate well-detectable ex vivo metabolic activity. Metabolic rates, in particular oxygen consumption rates have a tendency to decrease over time. Nonetheless, the impact of added drugs (doxorubicin, chloroacetaldehyde) is discriminable. Sensor-based platforms should be evaluated in explorative clinical studies for their suitability to support targeted systemic cancer therapy. Throughput is sufficient for testing various drugs in a range of concentrations while the information content obtained from multiparametric real-time analysis is superior to conventional endpoint assays.

Keywords

Targeted therapy Sensor Cancer tissue Cell metabolism 

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Copyright information

© International Federation for Medical and Biological Engineering 2012

Authors and Affiliations

  • Regina Kleinhans
    • 1
  • Martin Brischwein
    • 1
    Email author
  • Pei Wang
    • 1
  • Bernhard Becker
    • 1
  • Franz Demmel
    • 1
  • Tobias Schwarzenberger
    • 1
  • Marlies Zottmann
    • 1
  • Peter Wolf
    • 1
    • 2
  • Axel Niendorf
    • 3
  • Bernhard Wolf
    • 1
    Email author
  1. 1.Heinz Nixdorf-Lehrstuhl für Medizinische ElektronikTechnische Universität MünchenMunichGermany
  2. 2.HP Medizintechnik GmbHOberschleißheimGermany
  3. 3.Pathologie Hamburg-WestHamburgGermany

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