Can we see epithelium tissue structure below the surface using an optical probe?
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This paper answers the question of whether it is possible to detect changes below the surface in epithelium layered structures using a Stochastic Decomposition Method (SDM) that models the scattered light reflected from the layered structure over an area (2-D scan) illuminated by an optical sensor (fibre) emitting light at either one wavelength or with white light. Our technique correlates the differential changes in the reflected tissue texture with the morphological and physical changes that occur in the tissue occurring inside the structure. This work has great potential for detecting changes in mucosal structures and may lead to enhanced endoscopy when the disease is developing to the outside of the mucosal structure and hence becoming hidden during colonoscopy or endoscopic examination. Tests are performed on layered tissue phantoms, and the results obtained show great effectiveness of the model and method in picking up changes in the morphology of the layered tissue phantoms occurring below the surface. We also establish the robustness of the model to changes in viewing depth by testing it on phantoms viewed at different depths. We show that the model is robust to within a 4-mm-deep viewing range.
KeywordsBiological signal processing Optical imaging Pattern recognition Optical signal processing Stochastic analysis Spectral analysis Computer-aided diagnosis Modelling biomedical systems
We would like to thank Photonics Lab members: Ms. Elina Vitol, Dr. Timothy Kurzweg and Dr. Bahram Nabet in the ECE Dept. at Drexel University for providing the optical device (probe, spectrometer and light source) and the phantoms used in this research. Special thanks are also due Ms. Elina Vitol for preparing the multi-layered tissue phantoms. We also thank Dr. Jim Reynolds from Drexel College of Medicine for many useful discussions on the subject of light endoscopy.
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