Medical & Biological Engineering & Computing

, Volume 45, Issue 1, pp 91–97 | Cite as

Decrease in extracellular collagen crosslinking after NMR magnetic field application in skin fibroblasts

  • I. Digel
  • E. Kurulgan
  • Pt. Linder
  • P. Kayser
  • D. Porst
  • G. J. Braem
  • K. Zerlin
  • G. M. Artmann
  • A. Temiz Artmann
Original Article
First Online:
Received:
Accepted:

Abstract

Although biological effects of electromagnetic fields were investigated intensively, there is still no agreement on the significance of their effects. The underlying mechanisms and therapeutic importance are still mostly unknown too. In this study, primary cultures of human dermal fibroblasts were exposed to magnetic field at nuclear magnetic resonance (NMR) conditions for in total 5 days and 4 h/day. Among the investigated parameters were: cell proliferation rate, cell morphology, total protein concentration as well as content of skin-specific collagen types I, III, IV. NMR exposure induced distinct changes both in cellular and extracellular components. The extracellular matrix (ECM) of NMR-exposed cells had less cross-linked collagen. In particular, the increase of collagen of the soluble fraction was at 17.2 ± 2.9% for type I, 27.0 ± 1.86% for type III, 17.3 ± 1.46% for type IV (N = 6). In the absence of resonance frequency, the effects of magnetic field on ECM were less profound.

Keywords

Nuclear magnetic resonance Skin fibroblasts Collagen cross-linking 
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Copyright information

© International Federation for Medical and Biological Engineering 2006

Authors and Affiliations

  • I. Digel
    • 1
  • E. Kurulgan
    • 1
  • Pt. Linder
    • 1
  • P. Kayser
    • 1
  • D. Porst
    • 1
  • G. J. Braem
    • 1
  • K. Zerlin
    • 1
  • G. M. Artmann
    • 1
  • A. Temiz Artmann
    • 1
  1. 1.Department of Biomedical Engineering, Laboratory of Cell BiophysicsAachen University of Applied SciencesJuelichGermany

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