Food Biophysics

, Volume 9, Issue 3, pp 285–291 | Cite as

Competitive Adsorption of Lecithin and Saliva at the O/W Interface in Relation to the Oral Processing of Lipid Continuous Foods

  • Perrine Delime
  • Natasja Lemmens-Smink
  • Bettina WolfEmail author


This research is relevant to oral processing of lipid continuous foods. During this first step of food digestion, lipid continuous foods such as chocolate or margarine phase invert into oil-in-water emulsions stimulated through the mechanical action of tongue and teeth in combination with the change in temperature and the high surface activity of salivary proteins. These are hypothesised to stabilise the newly formed interface in competition with surfactants or surface active molecules released from the food if present. Here competitive adsorption between mechanically stimulated human whole saliva (HWS) and lecithin dissolved in sunflower oil freed of interfacially active contaminants was investigated in-vitro using a pendant drop tensiometer for dynamic interfacial tension and interfacial rheological measurements. Initially, it was validated that the interfacial properties of HWS samples remained unaffected by frozen storage at −80 °C during 6 weeks. Protein concentration affected the absolute values of interfacial tension and in particular the dilatational elastic modulus. Competitive adsorption studies revealed a mixed interface and it follows that emulsion stabilisation during oral processing involves both salivary proteins and lecithin present in the oil phase.


Oral processing Human whole saliva Interfacial rheology Competitive adsorption Lipid continuous foods Lecithin 



PD would like to thank Julia Maldonado-Valderrama for discussion and MARS NL is thanked for allowing this work to be published.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Perrine Delime
    • 1
  • Natasja Lemmens-Smink
    • 2
  • Bettina Wolf
    • 1
    Email author
  1. 1.Division of Food SciencesThe University of NottinghamLoughboroughUK
  2. 2.Mars Nederland B.V.VeghelNetherlands

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