LIBS-Based Detection of Antioxidant Elements in Seeds of Emblica officinalis
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The aim of the study was to determine the effect of the elements of the extract of seed from Emblica officinalis on antioxidant enzymes and osmotic fragility of erythrocytes membrane in normal as well as streptozotocin-induced severely diabetic albino Wister rats. The results revealed that the untreated diabetic rats exhibited increase in oxidative stress as indicated by significantly diminished activities of free radical scavenging enzymes such as catalase (CAT) and superoxide dismutase (SOD) by 37.5% (p < 0.001) and 18.6% (p < 0.01), respectively. However, the E. officinalis seed extract treatment showed marked improvements in CAT and SOD activities by 47.09% (p < 0.001) and 21.61% (p < 0.001), respectively. The enhanced lipid peroxidation by 30.87% (p < 0.001) in erythrocytes of untreated diabetic rats was significantly accentuated in the extract treated animals by 23.72% (p < 0.001). The erythrocytes showed increased osmotic fragility due to diabetes in terms of hemolysis. It attained the normal level in diabetic treated group. The findings thus suggest that E. officinalis seed extract has the potential to be exploited as an agent to boost the antioxidant system in the diabetic animal model. Laser-induced breakdown spectroscopy has been used as an analytical tool to detect major and minor elements like Mg, Fe, Na, K, Zn, Ca, H, O, C, and N present in the extract. The higher concentration of Ca (II), Mg (II) and Fe (II) as reflected by their intensities are responsible for the antioxidant potential of E. officinalis.
KeywordsEmblica officinalis Erythrocytes Diabetes Oxidative stress Osmotic fragility
The authors are grateful to BRNS, BARK Mumbai, India, for providing the financial assistance. First author Shikha Mehta is thankful to University Grants Commission (UGC), New Delhi, India for the award of SRF.
- 2.C. Watala, J. Golanski, M.A. Boncler, T. Pietrucha, K. Gwozdzinski, Platelets 9, 315–327 (1993)Google Scholar
- 4.C. Giulivi, K.J.A. Davis, J. Biol. Chem. 265, 19453–19460 (1990)Google Scholar
- 10.P.K. Rai, S.K. Singh, A.N. Kesari, G. Watal, Indian J. Med. Res. 126, 224–227 (2007)Google Scholar
- 22.D. Brahm, P. Trinder, Analyst 95, 142–145 (1972)Google Scholar
- 26.B.L. O’Dell, J.D. Browning, P.G. Reeves, J. Nutr. 117, 1883–1889 (1987)Google Scholar
- 27.C.K. Kokate, Preliminary phytochemical screening, in Practical pharmacognosy, ed. by C.K. Kokate (Vallabh Prakashan, New Delhi, 1994), pp. 107–113Google Scholar
- 28.J.B. Harborne, Methods of extraction and isolation, in Phytochemical methods, ed. by J.B. Harbone (Chapman and Hall, London, 1998), pp. 60–66Google Scholar
- 31.R. Kumar, N.V. Pillai, R. Kuttan, J. Exp. Clin. Cancer Res. 22, 201–206 (2003)Google Scholar
- 33.R. Ondreicka, I. Beno, O. Cerna, E. Granicova, M. Staruchowa, K. Volkova, P. Bobek, M. Tatara, Bratisl Lek Listy 99, 250–255 (1998)Google Scholar
- 35.B. Matkovies, M. Kotorman, I. Varga, D. Ouy Hai, C.S. Varga, Acta. Physiol. 85, 29–38 (1998)Google Scholar