Journal of Neuroimmune Pharmacology

, Volume 6, Issue 1, pp 158–162 | Cite as

n-Dodecyl-β-d-Maltoside Inhibits Aggregation of Human Interferon-β-1b and Reduces Its Immunogenicity

  • Robert A. RifkinEmail author
  • Edward T. Maggio
  • Sonny Dike
  • Douglas A. Kerr
  • Michael Levy


The development of neutralizing antibodies to the protein drug interferon-β is a significant impediment to its use in the treatment of multiple sclerosis. Neutralizing antibodies to interferon-β arise from aggregation of the peptide during manufacturing and storage. We tested the ability of dodecylmaltoside, a nontoxic alkylsaccharide surfactant, to reduce aggregation of interferon-β in vitro and to reduce its immunogenicity in vivo. Interferon-β, in solution with and without dodecylmaltoside, was periodically evaluated for aggregation by light scatter for 1 month. Interferon-β, with and without dodecylmaltoside, was given 3 days/week for 1 month to mice; the sera of these mice were analyzed for anti-interferon-β antibodies by ELISA. Dodecylmaltoside reduces the aggregation of interferon-β in vitro and its immunogenicity in vivo. Our positive findings warrant additional tests of dodecylmaltoside as a therapeutic adjuvant in rodent models of multiple sclerosis.


multiple sclerosis interferon-beta excipients disaccharides/chemistry 


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Robert A. Rifkin
    • 1
    Email author
  • Edward T. Maggio
    • 2
  • Sonny Dike
    • 1
  • Douglas A. Kerr
    • 3
  • Michael Levy
    • 1
  1. 1.Department of NeurologyThe Johns Hopkins University School of MedicineBaltimoreUSA
  2. 2.Aegis Therapeutics, LLCSan DiegoUSA
  3. 3.Biogen Idec, IncCambridgeUSA

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