Studies in the Modulation of Experimental Autoimmune Encephalomyelitis
Experimental autoimmune encephalomyelitis (EAE), an experimental model for multiple sclerosis, can be induced through inoculation with several different central nervous system (CNS) proteins or peptides. Modulation of EAE, resulting in either protection from EAE or enhancement of EAE, can also be accomplished through either vaccination or DNA immunization with molecular mimics of self-CNS proteins. Previously published data on this method of EAE modulation will be reviewed. New data is presented, which demonstrates that EAE can also be modulated through the administration of the β-(1,3)-d-glucan, curdlan. Dendritic cells stimulated by curdlan are involved in the differentiation of the interleukin-17 producing subset of CD4+ T cells that are recognized effector cells in EAE. Using two different systems to study the effects of curdlan on EAE, it was found that curdlan increased the incidence of EAE and/or the severity of the disease course.
Keywordsmyelin basic protein myelin proteolipid protein myelin oligodendrocyte glycoprotein vaccination DNA immunization interleukin-17
We wish to thank Nikki J. Kirkman, BS, Faris Hasanovic, BS, Daniel J. Doty, and Krystal D. Porter, BS, for the excellent technical assistance. We wish to acknowledge Kathleen Borick for the outstanding preparation of the manuscript.
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