, Volume 13, Issue 6, pp 2239–2248 | Cite as

Optical Detection of Thiol Drugs by Core–Shell Luminous Carbon Dots—Gold Nanoparticles System

  • Akansha Mehta
  • Amit Mishra
  • Soumen Basu


Nowadays, carbon quantum dots (CQDs) with size less than 10 nm have emerged as one of the most exciting areas of chemical research in the class of inorganic nanomaterials. This article presents interesting characteristics of CQDs and their fluorescence turn-on/turn-off quenching for the detection of 6-Thioguanine (6-TG). The CQDs were fabricated by a simple one-step microwave technique and used for the simultaneous reduction of Au3+ to form Au0-CQD core–shell (Au@CQD) nanocomposites. The CQDs formed were spherical in shape having an average size of ~ 7 nm confirmed by high-resolution transmission electron microscopy (HRTEM) and DLS study. The interaction of CQDs with Au leads to its fluorescence turn-off up to 96% analyzed by UV-visible, fluorescence spectroscopy, and fluorescence lifetime measurements. The turn-on fluorescence of CQDs has been witnessed by the formation of complex with 6-TG [Au-(6-TG)n]3− in the presence of thiols. Meanwhile, linear relationship between turn-on fluorescence against the concentration of 6-TG is obtained in the range of 0–100 μM with the correlation coefficient of 0.9944 and limit of detection for 6-TG has been found to be 0.01 μM. The Au@CQDs could also act as biosensor for the detection of various amino acids, enzymes, and pentids drug.

Graphical Abstract


Carbon quantum dots Fluorescence quenching 6-Thioguanine Turn-on/turn-off detection 



Authors are also grateful to Mr. Rayees Ahmad, School of Chemistry and Biochemistry, Thapar University, for the discussion and suggestions.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11468_2018_744_MOESM1_ESM.docx (390 kb)
ESM 1 (DOCX 390 kb)


  1. 1.
    Bunz UH, Rotello VM (2010) Gold nanoparticle–fluorophore complexes: sensitive and discerning “noses” for biosystems sensing. Angew Chem Int Ed 49(19):3268–3279CrossRefGoogle Scholar
  2. 2.
    Descalzo AB, Martínez-Máñez R, Sancenon F, Hoffmann K, Rurack K (2006) The supramolecular chemistry of organic–inorganic hybrid materials. Angew Chem Int Ed 45(36):5924–5948CrossRefGoogle Scholar
  3. 3.
    Yuan X, Tay Y, Dou X, Luo Z, Leong DT, Xie J (2013) Glutathione-protected silver nanoclusters as cysteine-selective fluorometric and colorimetric probe. Anal Chem 85(3):1913–1919CrossRefPubMedGoogle Scholar
  4. 4.
    Hu B, Zhao Y, Zhu H-Z, Yu S-H (2011) Selective chromogenic detection of thiol-containing biomolecules using carbonaceous nanospheres loaded with silver nanoparticles as carrier. ACS Nano 5(4):3166–3171CrossRefPubMedGoogle Scholar
  5. 5.
    Wang J, Qu X (2013) Recent progress in nanosensors for sensitive detection of biomolecules. Nano 5(9):3589–3600Google Scholar
  6. 6.
    Chen W-Y, Huang C-C, Chen L-Y, Chang H-T (2014) Self-assembly of hybridized ligands on gold nanodots: tunable photoluminescence and sensing of nitrite. Nano 6(19):11078–11083Google Scholar
  7. 7.
    Goesmann H, Feldmann C (2010) Nanoparticulate functional materials. Angew Chem Int Ed 49(8):1362–1395CrossRefGoogle Scholar
  8. 8.
    Daniel M-C, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104(1):293–346CrossRefPubMedGoogle Scholar
  9. 9.
    Mehta A, Sharma M, Kumar A, Basu S Effect of Au content on the enhanced photocatalytic efficiency of mesoporous Au/TiO2 nanocomposites in UV and sunlight. Gold Bulletin:1–9Google Scholar
  10. 10.
    Mehta A, Sharma M, Kumar A, Basu S (2016) Gold nanoparticles grafted mesoporous silica: a highly efficient and recyclable heterogeneous catalyst for reduction of 4-nitrophenol. Nano 11(09):1650104CrossRefGoogle Scholar
  11. 11.
    Liu D, Wang Z, Jiang X (2011) Gold nanoparticles for the colorimetric and fluorescent detection of ions and small organic molecules. Nano 3 (4):1421–1433CrossRefPubMedGoogle Scholar
  12. 12.
    Amjadi M, Farzampour L (2014) Fluorescence quenching of fluoroquinolones by gold nanoparticles with different sizes and its analytical application. J Lumin 145:263–268CrossRefGoogle Scholar
  13. 13.
    Liu Y, Zhou Q, Yuan Y, Wu Y (2017) Hydrothermal synthesis of fluorescent carbon dots from sodium citrate and polyacrylamide and their highly selective detection of lead and pyrophosphate. Carbon 115:550–560CrossRefGoogle Scholar
  14. 14.
    Hu R, Li L, Jin WJ (2017) Controlling speciation of nitrogen in nitrogen-doped carbon dots by ferric ion catalysis for enhancing fluorescence. Carbon 111:133–141CrossRefGoogle Scholar
  15. 15.
    Amjadi M, Abolghasemi-Fakhri Z, Hallaj T (2015) Carbon dots-silver nanoparticles fluorescence resonance energy transfer system as a novel turn-on fluorescent probe for selective determination of cysteine. J Photochem Photobiol A Chem 309:8–14CrossRefGoogle Scholar
  16. 16.
    Liu ML, Chen BB, Liu ZX, Huang CZ (2016) Highly selective and sensitive detection of 2, 4, 6-trinitrophenol by using newly developed blue–green photoluminescent carbon nanodots. Talanta 161:875–880CrossRefPubMedGoogle Scholar
  17. 17.
    Huang H, Liao L, Xu X, Zou M, Liu F, Li N (2013) The electron-transfer based interaction between transition metal ions and photoluminescent graphene quantum dots (GQDs): a platform for metal ion sensing. Talanta 117:152–157CrossRefPubMedGoogle Scholar
  18. 18.
    Zhao HX, Liu LQ, De Liu Z, Wang Y, Zhao XJ, Huang CZ (2011) Highly selective detection of phosphate in very complicated matrixes with an off–on fluorescent probe of europium-adjusted carbon dots. Chem Commun 47(9):2604–2606CrossRefGoogle Scholar
  19. 19.
    Li H, He X, Kang Z, Huang H, Liu Y, Liu J, Lian S, Tsang CHA, Yang X, Lee ST (2010) Water-soluble fluorescent carbon quantum dots and photocatalyst design. Angew Chem Int Ed 49(26):4430–4434CrossRefGoogle Scholar
  20. 20.
    Liu R, Huang H, Li H, Liu Y, Zhong J, Li Y, Zhang S, Kang Z (2013) Metal nanoparticle/carbon quantum dot composite as a photocatalyst for high-efficiency cyclohexane oxidation. ACS Catal 4(1):328–336CrossRefGoogle Scholar
  21. 21.
    Luo P, Li C, Shi G (2012) Synthesis of gold@ carbon dots composite nanoparticles for surface enhanced Raman scattering. Phys Chem Chem Phys 14(20):7360–7366CrossRefPubMedGoogle Scholar
  22. 22.
    Mandani S, Sharma B, Dey D, Sarma TK (2015) Carbon nanodots as ligand exchange probes in Au@ C-dot nanobeacons for fluorescent turn-on detection of biothiols. Nano 7(5):1802–1808Google Scholar
  23. 23.
    Köstereli Z, Severin K (2015) Array-based sensing of purine derivatives with fluorescent dyes. Org Biomol Chem 13(35):9231–9235CrossRefPubMedGoogle Scholar
  24. 24.
    Amjadi M, Shokri R, Hallaj T (2016) Interaction of glucose-derived carbon quantum dots with silver and gold nanoparticles and its application for the fluorescence detection of 6-thioguanine. LuminescenceGoogle Scholar
  25. 25.
    Sweetman SC (2009) Martindale: the complete drug reference. Pharmaceutical press,Google Scholar
  26. 26.
    Niu W-J, Shan D, Zhu R-H, Deng S-Y, Cosnier S, Zhang X-J (2016) Dumbbell-shaped carbon quantum dots/AuNCs nanohybrid as an efficient ratiometric fluorescent probe for sensing cadmium (II) ions and L-ascorbic acid. Carbon 96:1034–1042CrossRefGoogle Scholar
  27. 27.
    Li L, Wang C, Luo J, Guo Q, Liu K, Liu K, Zhao W, Lin Y (2015) Fe 3+−functionalized carbon quantum dots: a facile preparation strategy and detection for ascorbic acid in rat brain microdialysates. Talanta 144:1301–1307CrossRefPubMedGoogle Scholar
  28. 28.
    He D, Zheng C, Wang Q, He C, Lee Y-I, Wu L, Hou X (2015) Dielectric barrier discharge-assisted one-pot synthesis of carbon quantum dots as fluorescent probes for selective and sensitive detection of hydrogen peroxide and glucose. Talanta 142:51–56CrossRefPubMedGoogle Scholar
  29. 29.
    Pan J, Zheng Z, Yang J, Wu Y, Lu F, Chen Y, Gao W (2017) A novel and sensitive fluorescence sensor for glutathione detection by controlling the surface passivation degree of carbon quantum dots. TalantaGoogle Scholar
  30. 30.
    Gu J, Hu D, Wang W, Zhang Q, Meng Z, Jia X, Xi K (2015) Carbon dot cluster as an efficient “off–on” fluorescent probe to detect Au (III) and glutathione. Biosens Bioelectron 68:27–33CrossRefPubMedGoogle Scholar
  31. 31.
    Hou J, Zhang F, Yan X, Wang L, Yan J, Ding H, Ding L (2015) Sensitive detection of biothiols and histidine based on the recovered fluorescence of the carbon quantum dots–Hg (II) system. Anal Chim Acta 859:72–78PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Chemistry and BiochemistryThapar UniversityPatialaIndia

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