Aptamers are DNA or RNA single-stranded molecules that bind specifically to target molecules with high affinity. Function of nucleic acid aptamers is based on organized tertiary structure of them that is related to primary sequence, length of nucleic acid molecule, and environmental conditions. Herein, a localized surface plasmon resonance (LSPR) nanobioprobe has been developed based on specific aptamer-conjugated gold nanoparticles for rapid detection of methamphetamine. Detection of methamphetamine was studied via monitoring the gold nanoparticles (GNPs) LSPR band alterations in the presence of different concentrations. The covalent conjugation has been confirmed with FT-IR spectroscopy, and size alterations of gold nanoparticles before and after the conjugation state were monitored using dynamic light scattering (DLS) technique. The results show high affinity of aptamer to methamphetamine. Moreover, the results show conjugated aptamer with GNP in different concentrations of methamphetamine that contribute to color changes that is visible with unaided eye. Also, 14 nm LSPR shift was seen after conjugation of aptamer with GNP. Nanoparticle diameter after conjugation with aptamer was increased from 30 to 91 nm and decreased after incubation with methamphetamine (due to folding) from 91 to 84 nm. Detection limit of this designed nanoprobe is 500 nM. Plasmonic nanoparticle-based nanobioprobe is a new field for development of sensitive detection systems.
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We (all authors) have no conflict of interest.
Petit A, Karila L, Chalmin F, Lejoyeux M (2012) Methamphetamine addiction: a review of the literature. J Addict Res Ther 2012Google Scholar
Prickril B, Rasooly A (2017) Biosensors and biodetection. Vol 1. Optical-based detectors. Homana press, New York CityCrossRefGoogle Scholar
Prickril B, Rasooly A (2017) Biosensors and biodetection. Vol 2. Electrochemical, bioelectronic, piezoelectric, cellular and molecular biosensors. Homana press, New York CityGoogle Scholar
Banerjee J, Nilsen-Hamilton M (2013) Aptamers: multifunctional molecules for biomedical research. J Mol Med 91:1333–1342CrossRefPubMedGoogle Scholar
Ebrahimi M, Hamzeiy H, Barar J, Barzegari A, Omidi Y (2013) Systematic evolution of ligands by exponential enrichment selection of specific aptamer for sensing of methamphetamine. Sens Lett 11:566–570CrossRefGoogle Scholar
Ebrahimi M, Johari-Ahar M, Hamzeiy H, Barar J, Mashinchian O, Omidi Y (2012) Electrochemical impedance spectroscopic sensing of methamphetamine by a specific aptamer. BioImpacts : BI 2:91–95PubMedGoogle Scholar
Daniel M-C, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104:293–346CrossRefPubMedGoogle Scholar
Zayats M, Baron R, Popov I, Willner I (2005) Biocatalytic growth of Au nanoparticles: from mechanistic aspects to biosensors design. Nano Lett 5:21–25CrossRefPubMedGoogle Scholar
Saha K, Agasti SS, Kim C, Li X, Rotello VM (2012) Gold nanoparticles in chemical and biological sensing. Chem Rev 112:2739–2779CrossRefPubMedGoogle Scholar
Scarano S, Mascini M, Turner AP, Minunni M (2010) Surface plasmon resonance imaging for affinity-based biosensors. Biosens Bioelectron 25:957–966CrossRefPubMedGoogle Scholar
Azizi A, Ranjbar B, Moghadam TT, Bagheri Z, Baglou SR (2014) Surface plasmon resonance coupled circular dichroism of DNA–gold nanorods assembly. J Phys D Appl Phys 47:315401CrossRefGoogle Scholar
Lu Y, Liu Y, Zhang S, Wang S, Zhang S, Zhang X (2013) Aptamer-based plasmonic sensor array for discrimination of proteins and cells with the naked eye. Anal Chem 85:6571–6574CrossRefPubMedGoogle Scholar
Sato K, Hosokawa K, Maeda M (2007) Colorimetric biosensors based on DNA-nanoparticle conjugates. Anal Sci 23:17–20CrossRefPubMedGoogle Scholar
Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ (1996) A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382:607–609CrossRefPubMedGoogle Scholar
Hurst SJ, Lytton-Jean AK, Mirkin CA (2006) Maximizing DNA loading on a range of gold nanoparticle sizes. Anal Chem 78:8313–8318CrossRefPubMedGoogle Scholar
Huo F, Lytton-Jean AK, Mirkin CA (2006) Asymmetric functionalization of nanoparticles based on thermally addressable DNA interconnects. Adv Mater 18:2304–2306CrossRefGoogle Scholar
Moghadam TT, Ranjbar B (2016) Fabrication of aptamer-functionalized gold nanorod biosensor for colorimetric detection of lysozyme, une. 13:15Google Scholar
Chang C-C, Chen C-Y, Zhao X, Wu T-H, Wei S-C, Lin C-W (2014) Label-free colorimetric aptasensor for IgE using DNA pseudoknot probe. Analyst 139:3347–3351CrossRefPubMedGoogle Scholar