, Volume 9, Issue 3, pp 677–683 | Cite as

Carbohydrate–Lectin Interaction on Graphene-Coated Surface Plasmon Resonance (SPR) Interfaces

  • Abra Penezic
  • Geetanjali Deokar
  • Dominique Vignaud
  • Emmanuelle Pichonat
  • Henri Happy
  • Palaniappan Subramanian
  • Blaženka Gasparović
  • Rabah Boukherroub
  • Sabine SzuneritsEmail author


The paper describes the detection of carbohydrate–lectin interaction on graphene-on-metal surface plasmon resonance (SPR) interfaces. Graphene-coated gold-based SPR interfaces were formed through the transfer of large-area graphene grown by chemical vapor deposition (CVD) on polycrystalline Cu foils. The method allowed successful transfer of single- and double-layered graphene sheets onto the SPR interfaces in a reproducible manner. Functionalization of the graphene interface with mannose was achieved by simple immersion into a mannose aqueous solution (100 mM), resulting in noncovalent interactions between the aromatic ring structure of graphene and mannose. The utility of the carbohydrate-modified graphene-on-gold interface for the selective and sensitive detection of carbohydrate–lectin interactions was demonstrated using model lectins from Lens culinaris (LC) and Triticum vulgaris (TV). While LC lectin binds specifically to mannopyranoside units, TV lectin has an affinity for N-acetyl glucosamine and sialic acid residues.


Graphene Surface plasmon resonance Mannose Lectins Biosensors 



R.B. and S.S. gratefully acknowledge the financial support from the Centre National de Recherche Scientifique (CNRS), the University Lille 1, Nord Pas de Calais region, and the European Regional Development Fund (INTERREG IVa FW1.1.9 “Plasmobio” project). S.S. thanks the Institut Universitaire de France (IUF) for the financial support. P.S. gratefully acknowledges the financial support from the European Commission through RTN project MATCON (contract no. 238201). This work was partly funded by a grant from the Croatian Ministry of Science, Education and Sports, project 098-0982934-2717. Financial support from the ANR P2N GRACY project is acknowledged.


  1. 1.
    Mercey E, Sadir R, Maillart E, Roget A, Baleux F, Lortat-Jacob H, Livache T (2008) Anal Chem 80:3476CrossRefGoogle Scholar
  2. 2.
    Szunerits S, Niedziolka-Jonsson J, Boukherroub R, Woisel P, Baumann J-S, Siriwardena A (2010) Anal Chem 82:8203–8210CrossRefGoogle Scholar
  3. 3.
    Norberg O, Deng L, Yan M, Ramstrom O (2009) Bioconjugate Chem 20:2364–2370Google Scholar
  4. 4.
    Maalouli N, Barras A, Siriwardena A, Bouazaoui M, Boukherroub R, Szunerits S (2013) Analyst 138:805CrossRefGoogle Scholar
  5. 5.
    Wang H-K, Zhang Y, Yuan X, Chen Y, Yan M (2011) Bioconjugate Chem 22:26Google Scholar
  6. 6.
    Yonzon CR, Jeoung E, Zhou S, Schatz GC, Mrksich M, Van Duyne RP (2004) J Am Chem Soc 126:12669CrossRefGoogle Scholar
  7. 7.
    Mizukami K, Takakura H, Matsunaga T, Kitano H (2008) Colloids Surf B: Biointerfaces 66:110CrossRefGoogle Scholar
  8. 8.
    Smith EA, Thomas WD, Kiessling LL, Corn RM (2003) J Am Chem Soc 125:6140–6148CrossRefGoogle Scholar
  9. 9.
    Linman MJ, Taylor JD, Yu H, Chen X, Cheng Q (2008) Anal Chem 80:4007CrossRefGoogle Scholar
  10. 10.
    Housemann BT, Mrksich M (2002) Chem Biol 9:443CrossRefGoogle Scholar
  11. 11.
    Sun W-L, Stabler CL, Cazalis CS, Chaikof EL (2006) Bioconjugate Chem 17:52Google Scholar
  12. 12.
    Norberg O, Deng L, Aastrup T, Yan M, Ramstrom O (2011) Anal Chem 83:1000CrossRefGoogle Scholar
  13. 13.
    Norberg O, Lee IH, Aastrup T, Yan M, Ramstrom O (2012) Biosens Bioelectron 34:51CrossRefGoogle Scholar
  14. 14.
    Choi SH, Kim YL, Byun KM (2010) Opt Express 19:458CrossRefGoogle Scholar
  15. 15.
    Wu L, Chu H-S, Koh WS, Li EP (2010) Opt Express 18:14395CrossRefGoogle Scholar
  16. 16.
    Huh S, Park J, Kim KS, Hong BH, Kim SB (2011) ACS Nano 5:3639CrossRefGoogle Scholar
  17. 17.
    Salihoglu O, Balci O, Kocabas C (2011) Appl Phys Lett 100:213110CrossRefGoogle Scholar
  18. 18.
    Szunerits S, Maalouli N, Wijaya E, Vilcot JP, Boukherroub R (2013) Anal Bioanal Chem 405:1435CrossRefGoogle Scholar
  19. 19.
    Sing VV, Gupta G, Batra A, Nigam AK, Boopathi M, Gutch PK, Tripathi BK, Srivastava A, Samuel M, Agarwal GS, Sing B, Vijayaraghavan R (2012) Adv Funct Mater 22:2352CrossRefGoogle Scholar
  20. 20.
    Mao Y, Bao Y, Wang W, Li Z, Li F, Niu L (2011) Talanta 85:2106CrossRefGoogle Scholar
  21. 21.
    Wang L, Zhu C, Han L, Jin L, Zhou M, Dong S (2011) Chem Commun 47:7794CrossRefGoogle Scholar
  22. 22.
    Subramanian P, Lesniewski A, Kaminska I, Vlandas A, Vasilescu A, Niedziolka-Jonsson J, Pichonat E, Happy H, Boukherroub R, Szunerits S (2013) Biosens Bioelectron 50:239–243CrossRefGoogle Scholar
  23. 23.
    Kaminska I, Barras A, Coffinier Y, Lisowski W, Niedziolka-Jonsson J, Woisel P, Lyskawa J, Opallo M, Siriwardena A, Boukherroub R, Szunerits S (2012) ACS Appl Mater Interfaces 4:5386CrossRefGoogle Scholar
  24. 24.
    Wink T, Van Zuilen SJ, Bult A, Van Bennekom WP (1998) Anal Chem 70:827CrossRefGoogle Scholar
  25. 25.
    Kooyman RPH, Lenferink ATM, Eenik RG, Greve J (1991) Anal Chem 63:83CrossRefGoogle Scholar
  26. 26.
    Oznuluer T, Pince E, Polat EO O, Balci O, Salihoglu O, Kocabas C (2011) Appl Phys Lett 98:183101CrossRefGoogle Scholar
  27. 27.
    Li X, Cai W, An J, Kim S, Nah J, Yang D, Piner R, Velamakanni A, Jung I, Tutuc E, Banerjee SK, Colombo L, Ruoff RS (2009) Science 324:1312–1314CrossRefGoogle Scholar
  28. 28.
    Kaminska I, Das MR, Coffinier Y, Niedziolka-Jonsson J, Woisel P, Opallo M, Szunerits S, Boukherroub R (2012) Chem Commun 48:1221CrossRefGoogle Scholar
  29. 29.
    Wang JL, Zhou HS (2008) Anal Chem 80:7174CrossRefGoogle Scholar
  30. 30.
    Park S-M, Lee MR, Shin I (2009) Bioconjug Chem 20:155CrossRefGoogle Scholar
  31. 31.
    Kussrow A, Kaltgrad E, Wolfenden ML, Cloninger MJ, Finn MG, Bornhop DJ (2009) Anal Chem 81:4889CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Abra Penezic
    • 1
    • 2
  • Geetanjali Deokar
    • 3
  • Dominique Vignaud
    • 3
  • Emmanuelle Pichonat
    • 3
  • Henri Happy
    • 3
  • Palaniappan Subramanian
    • 1
  • Blaženka Gasparović
    • 2
  • Rabah Boukherroub
    • 1
  • Sabine Szunerits
    • 1
    Email author
  1. 1.Institut de Recherche Interdisciplinaire (IRI, USR-3078)Université Lille 1Villeneuve d’AscqFrance
  2. 2.Division for Marine and Environmental ResearchRuđer Bošković InstituteZagrebCroatia
  3. 3.Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520Université Lille 1Villeneuve d’AscqFrance

Personalised recommendations