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Plasmonics

, Volume 9, Issue 4, pp 801–807 | Cite as

Multiplexed Biomolecular Detection Based on Single Nanoparticles Immobilized on Pneumatically Controlled Microfluidic Chip

  • Bo Wu
  • Li-Chan Chen
  • Youju Huang
  • Yiming Zhang
  • Yuejun Kang
  • Dong-Hwan KimEmail author
Article

Abstract

A microfluidic chip integrated with pneumatically controlled valves was developed for multiplexed biomolecular detection via localized surface plasmonic resonance (LSPR) of single gold nanorod. The cost-effective microfluidic chip was assembled by polydimethylsiloxane layers and glass substrates with a precisely controlled thickness. The thin and flat microfluidic chip fitted the narrow space of dark-field microscopy and enabled the recording of single-nanoparticle LSPR responses. Aptamer-antigen-antibody sandwiched detection scheme was utilized to enhance the LSPR responses for label-free biomolecular detection. This microfluidic chip successfully demonstrated the multiplexed detection of three independent analytes (PSA, IgE, and thrombin).

Keywords

LSPR Single nanoparticle Multiplex Microfluidic chip 

Notes

Acknowledgments

We gratefully thank financial support from the Ministry of Education of Singapore (MOE2012-T2-1-058).

Supplementary material

11468_2013_9661_MOESM1_ESM.docx (3.2 mb)
ESM 1 (DOCX 3,285 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Bo Wu
    • 1
  • Li-Chan Chen
    • 1
  • Youju Huang
    • 1
  • Yiming Zhang
    • 1
  • Yuejun Kang
    • 1
  • Dong-Hwan Kim
    • 1
    Email author
  1. 1.School of Chemical and Biomedical EngineeringNanyang Technological UniversitySingaporeSingapore

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