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Plasmonics

, Volume 6, Issue 1, pp 105–112 | Cite as

Application of Gold Nanorods for Plasmonic and Magnetic Imaging of Cancer Cells

  • Liwei Liu
  • Hong Ding
  • Ken-Tye Yong
  • Indrajit Roy
  • Wing-Cheung Law
  • Atcha Kopwitthaya
  • Rajiv Kumar
  • Folarin Erogbogbo
  • Xihe Zhang
  • Paras N. Prasad
Article

Abstract

We report the use of biocompatible gold nanorods (GNRs) as multimodal (plasmonic and magnetic) probes for cancer cell labeling in vitro. These multifunctional and multimodal bioconjugates were prepared by replacing cetyltrimethylammonium bromide with a mixture of functionalized PEGylation molecules so that a variety of functionalities (e.g., magnetic resonance imaging agent gadolinium (Gd) and biorecognition molecule transferrin (Tf)) can be easily integrated using simple chemistry. It was shown that Gd incorporation did not interfere with the plasmonic properties of the GNRs and a strong T1 relaxivity was estimated (10.0 mM−1 s−1), which is more than twice that of the clinical MRI agent Gd-DTPA. The large observed T1 relaxivity was possibly due to the huge surface to volume ratio of GNR, which allowed huge amount of amine-terminated molecule to anchor on the surface, coupled with Gd (III) ions for the enhanced relaxation of water protons. Pancreatic cancer cell overexpressing the transferring receptor was served as the in vitro model, and the Tf-mediated uptake was demonstrated and confirmed by dark-field imaging and transmission electron microscopy. More importantly, cell viability (MTS) assay did not reveal any sign of toxicity in these treated cells, suggesting that PEGylated GNRs can serve as a biocompatible, multifunctional, and multimodal platform for variable bio-applications.

Keywords

Gold nanorods Cancer Dark-field imaging MRI DOTA Gd 

Notes

Acknowledgments

This work was supported by grants from the National Cancer Institute/National Institutes for Health (RO1-CA-119397) and the start-up grant of the International Joint Research Center for Nanophotonics and Biophotonics (IJRCNB).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Liwei Liu
    • 1
    • 2
    • 3
  • Hong Ding
    • 2
    • 3
  • Ken-Tye Yong
    • 2
    • 3
  • Indrajit Roy
    • 2
    • 3
  • Wing-Cheung Law
    • 2
    • 3
  • Atcha Kopwitthaya
    • 2
  • Rajiv Kumar
    • 2
  • Folarin Erogbogbo
    • 2
  • Xihe Zhang
    • 1
    • 3
  • Paras N. Prasad
    • 2
    • 3
  1. 1.School of ScienceChangchun University of Science and TechnologyChangchunChina
  2. 2.The Institute for Lasers, Photonics and Biophotonics (ILPB)The State University of New York at BuffaloBuffaloUSA
  3. 3.International Joint Research Center for Nanophotonics and BiophotonicsChangchunChina

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