, Volume 4, Issue 2, pp 187–192 | Cite as

SERS Study of Tetrodotoxin (TTX) by Using Silver Nanoparticle Arrays

  • Wen-Chi Lin
  • Hsiao-Chin Jen
  • Chang-Long Chen
  • Deng-Fwu Hwang
  • Railing Chang
  • Jih-Shang Hwang
  • Hai-Pang Chiang


The optical properties of tetrodotoxin (TTX), a scarce toxin with anesthetic properties, were studied using nanoparticle arrays-assisted surface-enhanced Raman scattering (SERS). The nanoparticles arrays were fabricated using nanosphere lithography and a metallic lift-off process to control the particle size, shape, and spacing in the arrays. Using density functional methods, the Raman spectrum of TTX was also calculated with Gaussian03 software. The main peaks of the spectrum are originated from the vibration of the NH2 molecule group. In the SERS experiment, we were able to measure the Raman spectrum with a TTX concentration as less as 0.9 ng/mL. This sensitivity is comparable to that from high performance liquid chromatography.


Surface enhaced Raman scattering (SERS) Tetrodotoxin (TTX) Nanosphere lithography (NSL) Ag nanoparticle arrays Density functional theory (DFT) 


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Wen-Chi Lin
    • 1
  • Hsiao-Chin Jen
    • 2
  • Chang-Long Chen
    • 1
  • Deng-Fwu Hwang
    • 2
  • Railing Chang
    • 1
  • Jih-Shang Hwang
    • 1
  • Hai-Pang Chiang
    • 1
    • 3
  1. 1.Institute of Optoelectronic SciencesNational Taiwan Ocean UniversityKeelungTaiwan
  2. 2.Institute of Food SciencesNational Taiwan Ocean UniversityKeelungTaiwan
  3. 3.Institute of PhysicsAcademia SinicaTaipeiTaiwan

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