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Plasmonics

, Volume 2, Issue 3, pp 97–106 | Cite as

Long Range Surface Plasmons for Observation of Biomolecular Binding Events at Metallic Surfaces

  • Jakub DostálekEmail author
  • Amal Kasry
  • Wolfgang Knoll
Article

Abstract

A long range surface plasmon (LRSP) is an electromagnetic wave propagating along a thin metal film with an order of magnitude lower damping than conventional surface plasmon (SP) waves. Thus, the excitation of LRSP is associated with a narrower resonance and it provides larger enhancement of intensity of the electromagnetic field. In surface plasmon resonance (SPR) biosensors, these features allow a more precise observation of the binding of biomolecules in the proximity to the metal surface by using the (label-free) measurement of refractive index (RI) variations and by SP-enhanced fluorescence spectroscopy. In this contribution, we investigate LRSPs excited on a layer structure consisting of a fluoropolymer buffer layer, a thin gold film, and an aqueous sample. By implementing such structure in an SPR sensor, we achieved a 2.4- and 4.4-fold improvement of the resolution in the label-free and fluorescence-based detection, respectively, of the binding of biomolecules in the close proximity to the surface. Moreover, we demonstrate that the sensor resolution can be improved by a factor of 14 and 12 for the label-free and fluorescence-based detection, respectively, if the biomolecular binding events occur within the whole evanescent field of LRSP.

Keywords

Surface plasmon resonance Long range surface plasmon Biosensor Fluorescence spectroscopy Optical sensor 

Notes

Acknowledgements

Partial support for this work was provided by the Deutsche Forschungsgemainschaft (KN 224/18-1, Schwerpunktprogramm “Intelligente Hydrogele”, JPP 1259) and by the EU through FP6-2005-FOOD-036300 (“TRACEPACK”).

References

  1. 1.
    Rather H (1983) Surface plasmons on smooth and rough surfaces and on gratings. Springer, Berlin Heidelberg New YorkGoogle Scholar
  2. 2.
    Homola J, Yee SS, Gauglitz G (1999) Surface plasmon resonance sensors: review. Sens Actuators, B, Chem 54:3–15CrossRefGoogle Scholar
  3. 3.
    Liebermann T, Knoll W (2000) Surface-plasmon field-enhanced fluorescence spectroscopy. Colloids Surf, A Physicochem Eng Asp 171:115–130CrossRefGoogle Scholar
  4. 4.
    Sarid D (1981) Long-range surface-plasma waves on very thin metal-films. Phys Rev Lett 47:1927–1930CrossRefGoogle Scholar
  5. 5.
    Craig AE, Olson GA, Sarid D (1983) Experimental observation of the long-range surface-plasmon polariton. Opt Lett 8:380–382Google Scholar
  6. 6.
    Matsubara K, Kawata S, Minami S (1990) Multilayer system for a high-precision surface-plasmon resonance sensor. Opt Lett 15:75–77CrossRefGoogle Scholar
  7. 7.
    Nenninger GG, Tobiska P, Homola J, Yee SS (2001) Long–range surface plasmons for high–resolution surface plasmon resonance sensors, Sensors and Actuators B–Chemical 74:145–151CrossRefGoogle Scholar
  8. 8.
    Slavik S, Homola J (2007) Ultrahigh resolution long range surface plasmon-based sensor. Sens Actuators, B, Chem 123:10–12Google Scholar
  9. 9.
    Wark AW, Lee HJ, Corn RM (2005) Long-range surface plasmon resonance imaging for bioaffinity sensors. Anal Chem 77:3904–3907CrossRefGoogle Scholar
  10. 10.
    Kasry A, Knoll W (2006) Long range surface plasmon fluorescence spectroscopy. Appl Phys Lett 89:101106CrossRefGoogle Scholar
  11. 11.
    Knoll W, Zizlsperger M, Liebermann T, Arnold S, Badia A, Liley M, Piscevic D, Schmitt FJ, Spinke J (2000) Streptavidin arrays as supramolecular architectures in surface-plasmon optical sensor formats. Colloids Surf, A Physicochem Eng Asp 161:115–137CrossRefGoogle Scholar
  12. 12.
    Swann MJ, Peel LL, Carrington S, Freeman NJ (2004) Dual-polarization interferometry: an analytical technique to measure changes in protein structure in real time, to determine the stoichiometry of binding events, and to differentiate between specific and nonspecific interactions. Anal Biochem 329:190–198CrossRefGoogle Scholar
  13. 13.
    Malmsten M (1994) Ellipsometry studies of protein layers adsorbed at hydrophobic surfaces. J Colloid Interface Sci 166:333–342CrossRefGoogle Scholar
  14. 14.
    Homola J, Piliarik M (2006) SPR sensor instrumentation. In: Homola J (ed) Surface plasmon resonance based sensors. Springer, Berlin Heidelberg New York, pp 45–69CrossRefGoogle Scholar
  15. 15.
    Vasilev K, Knoll W, Kreiter M (2004) Fluorescence intensities of chromophores in front of a thin metal film. J Chem Phys 120:3439–3445CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Department of Materials ScienceMax Planck Institute for Polymer ResearchMainzGermany
  2. 2.Center for Cell Analysis and Modeling (CCAM)UConn Health CenterFarmingtonUSA

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