, Volume 2, Issue 3, pp 97–106 | Cite as

Long Range Surface Plasmons for Observation of Biomolecular Binding Events at Metallic Surfaces

  • Jakub DostálekEmail author
  • Amal Kasry
  • Wolfgang Knoll


A long range surface plasmon (LRSP) is an electromagnetic wave propagating along a thin metal film with an order of magnitude lower damping than conventional surface plasmon (SP) waves. Thus, the excitation of LRSP is associated with a narrower resonance and it provides larger enhancement of intensity of the electromagnetic field. In surface plasmon resonance (SPR) biosensors, these features allow a more precise observation of the binding of biomolecules in the proximity to the metal surface by using the (label-free) measurement of refractive index (RI) variations and by SP-enhanced fluorescence spectroscopy. In this contribution, we investigate LRSPs excited on a layer structure consisting of a fluoropolymer buffer layer, a thin gold film, and an aqueous sample. By implementing such structure in an SPR sensor, we achieved a 2.4- and 4.4-fold improvement of the resolution in the label-free and fluorescence-based detection, respectively, of the binding of biomolecules in the close proximity to the surface. Moreover, we demonstrate that the sensor resolution can be improved by a factor of 14 and 12 for the label-free and fluorescence-based detection, respectively, if the biomolecular binding events occur within the whole evanescent field of LRSP.


Surface plasmon resonance Long range surface plasmon Biosensor Fluorescence spectroscopy Optical sensor 



Partial support for this work was provided by the Deutsche Forschungsgemainschaft (KN 224/18-1, Schwerpunktprogramm “Intelligente Hydrogele”, JPP 1259) and by the EU through FP6-2005-FOOD-036300 (“TRACEPACK”).


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Department of Materials ScienceMax Planck Institute for Polymer ResearchMainzGermany
  2. 2.Center for Cell Analysis and Modeling (CCAM)UConn Health CenterFarmingtonUSA

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