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Science Bulletin

, Volume 61, Issue 8, pp 632–638 | Cite as

Super-resolution imaging and tracking of TGF-β receptor II on living cells

  • Zi Ye
  • Nan Li
  • Libo Zhao
  • Yahong Sun
  • Hefei Ruan
  • Mingliang Zhang
  • Jinghe Yuan
  • Xiaohong FangEmail author
Article Chemistry

Abstract

Single-particle tracking photoactivated localization microscopy (sptPALM) has recently emerged as a powerful tool for high-density imaging and tracking of individual molecules in living cells. In this work, we have monitored and compared the diffusion dynamics of TGF-β type II receptor (TβRII) at high expression level using both traditional single-particle tracking (SPT) and sptPALM. The ligand-induced aggregation of TβRII oligomers was further indicated by sptPALM. Due to the capacity of distinguishing and tracking single molecules within diffraction limit, sptPALM outperforms traditional SPT by providing more accurate biophysical information.

Keywords

Single-particle tracking Photoactivated localization microscopy Single-molecule fluorescence imaging TGF-β receptor II Membrane diffusion 

超分辨成像单分子示踪研究TGF-β二型受体的细胞膜上扩散运动

摘要

用于单粒子示踪的光活化定位显微镜(sptPALM)是研究活细胞中单分子高密度成像和追踪的一种新的有效工具。本文分别利用传统的单粒子追踪方法(SPT)和sptPALM对高表达的 转化生长因子 TGF-β二型受体(TβRII)在细胞膜上的扩散运动进行了表征和比较,同时用sptPALM证实配体刺激下TβRII寡聚体进一步聚集。由于sptPALM可区分并追踪处于衍射极限内的多个单分子,它优于传统的SPT方法,能提供更准确的生物分子动态变化信息。

关键词

单粒子追踪 光活化定位显微镜 单分子荧光成像 TGF-β二型受体 膜上扩散运动 

Notes

Acknowledgments

This work was supported by the National Basic Research Program of China (2013CB933701), the National Natural Science Foundation of China (21127901, 91413119, 91213305) and the Chinese Academy of Science.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11434_2016_1043_MOESM1_ESM.doc (208 kb)
Supplementary material 1 (DOC 208 kb)

Supplementary material 2 (MPG 3558 kb)

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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Zi Ye
    • 1
  • Nan Li
    • 1
  • Libo Zhao
    • 1
  • Yahong Sun
    • 1
  • Hefei Ruan
    • 1
  • Mingliang Zhang
    • 1
  • Jinghe Yuan
    • 1
  • Xiaohong Fang
    • 1
    Email author
  1. 1.Key Laboratory of Molecular Nanostructure and Nanotechnology, Beijing National Laboratory for Molecular Sciences, Institute of ChemistryChinese Academy of SciencesBeijingChina

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