Chinese Science Bulletin

, Volume 58, Issue 23, pp 2846–2854 | Cite as

Genome survey uncovers the secrets of sex and lifestyle in caterpillar fungus

  • Xiao Hu
  • YongJie Zhang
  • GuoHua Xiao
  • Peng Zheng
  • YongLiang Xia
  • XingYu Zhang
  • Raymond J. St Leger
  • XingZhong Liu
  • ChengShu Wang
Open Access
Article Genetics

Abstract

The caterpillar fungus Ophiocordyceps sinensis (best known as Cordyceps sinensis) mummifies ghost moth larvae exclusively in Tibetan Plateau alpine ecosystems. Touted as “Himalayan Viagra”, the fungus is highly prized due to its medical benefits and dwindling supplies. Attempts to culture the sexual fruiting-body have failed and the huge market demand has led to severe devastation of local ecosystems and to the fungus heading towards extinction. By genome sequencing, we establish that unlike related insect pathogens O. sinensis contains two compatible mating-type genes in its genome and is self-fertile, i.e. homothallic. However, sexual processes are only initiated under native environmental conditions. O. sinensis resembles biotrophic plant pathogens in having a genome shaped by retrotransposon-driven expansions. The resulting changes in gene content suggest that O. sinensis has a biphasic pathogenic mechanism beginning with stealth pathogenesis in early host instars. O. sinensis is the first psychrophilic fungus sequenced and is adapted to extreme cold with putative antifreeze proteins and mechanisms for increasing lipid accumulation and fatty acid unsaturation. We hypothesize that for the inbreeding O. sinensis the massive proliferation of retrotransposons provides a tradeoff between the advantages of increased genetic variation independent of sexual recombination and deletion of genes dispensable for its specialized pathogenic lifestyle.

Keywords

Ophiocordyceps sinensis genome expansion homothallism biotrophic parasitism psychrophile 

Supplementary material

11434_2013_5929_MOESM1_ESM.pdf (729 kb)
Supplementary material, approximately 728 KB.

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Copyright information

© The Author(s) 2013

Authors and Affiliations

  • Xiao Hu
    • 1
  • YongJie Zhang
    • 2
  • GuoHua Xiao
    • 1
  • Peng Zheng
    • 1
  • YongLiang Xia
    • 1
  • XingYu Zhang
    • 3
  • Raymond J. St Leger
    • 4
  • XingZhong Liu
    • 3
  • ChengShu Wang
    • 1
  1. 1.Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant physiology and Ecology, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina
  2. 2.School of Life SciencesShanxi UniversityTaiyuanChina
  3. 3.State Key Laboratory of Mycology, Institute of MicrobiologyChinese Academy of SciencesBeijingChina
  4. 4.Department of EntomologyUniversity of MarylandCollege ParkUSA

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