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Chinese Science Bulletin

, Volume 57, Issue 23, pp 3051–3058 | Cite as

Preparation and cytocompatibility of polylactic acid/hydroxyapatite/graphene oxide nanocomposite fibrous membrane

  • HaiBin Ma
  • WenXin Su
  • ZhiXin Tai
  • DongFei Sun
  • XingBin YanEmail author
  • Bin Liu
  • QunJi Xue
Open Access
Article Special Issue: Graphene

Abstract

A series of polylactic acid (PLA) based nanocomposite fibrous membranes, including neat PLA, PLA/hydroxyapatite (HA) and PLA/HA/graphene oxide (GO), were fabricated via electrospinning method. The morphology and composition were investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) respectively. The thermal stability was determined by thermogravimetric analysis (TGA). To estimate the cytocompatibility of asprepared PLA/HA/GO fibrous membrane, MC3T3-E1 cells were cultured, and the corresponding cell adhesion and differentiation capability were investigated by fluorescence microscopy, SEM and MTT test. The electrospun ternary PLA/HA/GO membrane exhibited three-dimensional fibrous structure with relatively rough surface morphology, which made itself ideal for cell attachment and proliferation in bone tissue regeneration. The fluorescence microscopy, SEM and MTT test confirmed that the PLA/HA/GO nanocomposite fibrous membrane created a proper environment for the seeding and proliferation of MC3T3-E1 cells.

Keywords

polylactic acid hydroxyapatite graphene oxide fibrous membranes 

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Copyright information

© The Author(s) 2012

Authors and Affiliations

  • HaiBin Ma
    • 1
    • 3
  • WenXin Su
    • 2
  • ZhiXin Tai
    • 1
  • DongFei Sun
    • 1
  • XingBin Yan
    • 1
    Email author
  • Bin Liu
    • 2
  • QunJi Xue
    • 1
  1. 1.State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhouChina
  2. 2.School of StomatologyLanzhou UniversityLanzhouChina
  3. 3.Gansu Provincial Hospital of Traditional Chinese MedicineLanzhouChina

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