Advertisement

Chinese Science Bulletin

, Volume 57, Issue 17, pp 2113–2119 | Cite as

A combination of leaf rust resistance gene Lr34 and lesion mimic gene lm significantly enhances adult plant resistance to Puccinia triticina in wheat

  • Tao LiEmail author
  • GuiHua BaiEmail author
  • ShiLiang Gu
Open Access
Article Plant Pathology

Abstract

Leaf rust caused by Puccinia triticina is an economically-important disease in wheat worldwide. A combination of different types of resistance genes may significantly enhance rust resistance under rust-favorable conditions. To investigate the interactions between the rust resistance gene Lr34 and the lesion mimic gene lm on 1BL in Ning 7840, a segregating F8-10 population of 180 recombinant inbred lines was developed from Ning 7840/Chokwang and evaluated for both lesion mimic expression and leaf rust response at the adult plant stage in a greenhouse. A major quantitative trait locus (QTL), derived from Sumai 3, was co-localized with Lr34 on chromosome 7D and explained 41.5% of phenotypic variations for rust severity and 22.1% for leaf tip necrosis (LTN). The presence of Lr34 was confirmed by Lr34-specific markers cssfr1 and cssfr2 in Ning 7840 and Sumai 3. Unlike Lr34, lm conditioned a spontaneous lesion mimic phenotype and had a significant effect on reducing uredinial size, and a smaller effect on severity. Additive effects were observed between lm and Lr34 for severity and LTN, and an epistatic effect was observed for infection type. Single marker analysis also identified several other QTL with minor effects on severity, infection type, or LTN.

Keywords

Triticum aestivum Puccinia triticina lesion mimic slow rusting gene Lr34 leaf rust resistance 

Supplementary material

11434_2012_5001_MOESM1_ESM.pdf (401 kb)
Supplementary material, approximately 400 KB.

References

  1. 1.
    Bolton M D, Kolmer J A, Garvin D F. Wheat leaf rust caused by Puccinia triticina. Mol Plant Pathol, 2008, 9: 563–575CrossRefGoogle Scholar
  2. 2.
    Gurr S J, Rushton P J. Engineering plants with increased disease resistance: What are we going to express? Trends Biotechnol, 2005, 23: 275–282CrossRefGoogle Scholar
  3. 3.
    Dyck P L. The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome, 1987, 29: 467–469CrossRefGoogle Scholar
  4. 4.
    Singh R P, Huerta-Espino J. Effect of leaf rust resistance gene Lr34 on components of slow rusting at seven growth stages in wheat. Euphytica, 2003, 129: 371–376CrossRefGoogle Scholar
  5. 5.
    Singh R P, Mujeeb-Kazi A, Huerta-Espino J. Lr46: A gene conferring slow-rusting resistance to leaf rust in wheat. Phytopathology, 1998, 88: 890–894CrossRefGoogle Scholar
  6. 6.
    Martinez F, Niks R E, Singh R P, et al. Characterization of Lr46, a gene conferring partial resistance to wheat leaf rust. Hereditas, 2001, 135: 111–114CrossRefGoogle Scholar
  7. 7.
    Dyck P, Amborski D, Anderson R. Inheritance of adult-plant leaf rust resistance derived from the common wheat varieties Exchange and Frontana. Can J Genet Cytol, 1966, 8: 665–671Google Scholar
  8. 8.
    Krattinger S G, Lagudah E S, Spielmeyer W, et al. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science, 2009, 323: 1360–1363CrossRefGoogle Scholar
  9. 9.
    Lorrain S, Vailleau F, Balague C, et al. Lesion mimic mutants: Keys for deciphering cell death and defense pathways in plants? Trends Plant Sci, 2003, 8: 263–271CrossRefGoogle Scholar
  10. 10.
    Campbell M A, Ronald P C. Characterization of four rice mutants with alterations in the defence response pathway. Mol Plant Pathol, 2005, 6: 11–21CrossRefGoogle Scholar
  11. 11.
    Johal G S, Hulbert S H, Briggs S P. Disease lesion mimics of maize: A model for cell death in plants. BioEssays, 1995, 17: 685–692CrossRefGoogle Scholar
  12. 12.
    Takahashi A, Kawasaki T, Henmi K, et al. Lesion mimic mutants of rice with alterations in early signaling events of defense. Plant J, 1999, 17: 535–545CrossRefGoogle Scholar
  13. 13.
    Yin Z C, Chen J, Zeng L R, et al. Characterizing rice lesion mimic mutants and identifying a mutant with broad-spectrum resistance to rice blast and bacterial blight. Mol Plant-Microbe Interact, 2000, 13: 869–876CrossRefGoogle Scholar
  14. 14.
    Jung Y H, Lee J H, Agrawal G K, et al. The rice (Oryza sativa) blast lesion mimic mutant, blm, may confer resistance to blast pathogens by triggering multiple defense-associated signaling pathways. Plant Physiol Biochem, 2005, 43: 397–406CrossRefGoogle Scholar
  15. 15.
    Greenberg J T, Ausubel F M. Arabidopsis mutants compromised for the control of cellular damage during pathogenesis and aging. Plant J, 1993, 4: 327–341CrossRefGoogle Scholar
  16. 16.
    Dietrich R, Delaney T, Uknes S, et al. Arabidopsis mutants simulating disease resistance response. Cell, 1994, 77: 565–577CrossRefGoogle Scholar
  17. 17.
    Rate D N, Cuenca J V, Bowman G R, et al. The gain-of-function Arabidopsis acd6 mutant reveals novel regulation and function of the salicylic acid signaling pathway in controlling cell death, defenses, and cell growth. Plant Cell, 1999, 11: 1695–1708Google Scholar
  18. 18.
    Li T, Bai G. Lesion mimic associates with adult plant resistance to leaf rust infection in wheat. Theor Appl Genet, 2009, 119: 13–21CrossRefGoogle Scholar
  19. 19.
    Boyd L, Minchin P. Wheat mutants showing altered adult plant disease resistance. Euphytica, 2001, 122: 361–368CrossRefGoogle Scholar
  20. 20.
    Boyd L, Smith P, Wilson A, et al. Mutations in wheat showing altered field resistance to yellow and brown rust. Genome, 2002, 45: 1035–1040CrossRefGoogle Scholar
  21. 21.
    Anand A, Schmelz E, Muthukrishnan S. Development of a lesion-mimic phenotype in a transgenic wheat line overexpressing genes for pathogenesis-related (PR) proteins is dependent on salicylic acid concentration. Mol Plant Microbe Interact, 2003, 16: 916–925CrossRefGoogle Scholar
  22. 22.
    Kamlofski C A, Antonelli E, Bender C, et al. A lesion-mimic mutant of wheat with enhanced resistance to leaf rust. Plant Pathol, 2007, 56: 46–54CrossRefGoogle Scholar
  23. 23.
    Peterson R F, Campbell A B, Hannah A E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J For Res, 1948, 26: 496–500Google Scholar
  24. 24.
    Roelfs A P, Singh R P, Saari E E. Rust Diseases of Wheat: Concepts and Methods of Disease Management. Mexico, D.F.: CIMMYT, 1992Google Scholar
  25. 25.
    McIntosh R A, Wellings C R, Park R F. Wheat Rusts, an Atlas of Resistance Genes. East Melbourne: CSIRO Publications, 1995. 200Google Scholar
  26. 26.
    Yu J B, Bai G H, Cai S B, et al. Marker-assisted characterization of Asian wheat lines for resistance to Fusarium head blight. Theor Appl Genet, 2006, 113: 308–320CrossRefGoogle Scholar
  27. 27.
    Lagudah E, Krattinger S, Herrera-Foessel S, et al. Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet, 2009, 119: 889–898CrossRefGoogle Scholar
  28. 28.
    Wang S, Basten C J, Zeng Z B. Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC, 2007Google Scholar
  29. 29.
    Schnurbusch T, Paillard S, Schori A, et al. Dissection of quantitative and durable leaf rust resistance in Swiss winter wheat reveals a major resistance QTL in the Lr34 chromosomal region. Theor Appl Genet, 2004, 108: 477–484CrossRefGoogle Scholar
  30. 30.
    Lagudah E S, McFadden H, Singh R P, et al. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet, 2006, 114: 21–30CrossRefGoogle Scholar
  31. 31.
    Kloppers F J, Pretorius Z A. Effects of combinations amongst genes Lr13, Lr34 and Lr37 on components of resistance in wheat to leaf rust. Plant Pathol, 1997, 46: 737–750CrossRefGoogle Scholar
  32. 32.
    Rubiales D, Niks R E. Characterization of Lr34, a major gene conferring nonhypersensitive resistance to wheat leaf rust. Plant Dis, 1995, 79: 1208–1212CrossRefGoogle Scholar
  33. 33.
    German S E, Kolmer J A. Effect of Gene Lr34 in the enhancement of resistance to leaf rust of wheat. Theor Appl Genet, 1992, 84: 97–105CrossRefGoogle Scholar
  34. 34.
    Singh R P, Gupta A K. Expression of wheat leaf rust resistance gene Lr34 in seedlings and adult plants. Plant Dis, 1992, 76: 489–491CrossRefGoogle Scholar
  35. 35.
    Singh R P, William H M, Huerta-Espino J, et al. Wheat rust in Asia: Meeting the challenges with old and new technologies. In: Proceedings of the 4th International Crop Science Congress. Brisbane, Australia. 2004Google Scholar
  36. 36.
    Liu S, Zhang X, Pumphrey M O, et al. Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat. Funct Integr Genomics, 2006, 6: 83–89CrossRefGoogle Scholar

Copyright information

© The Author(s) 2012

Authors and Affiliations

  1. 1.Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology; Key Laboratory of Plant Functional Genomics of Ministry of EducationYangzhou UniversityYangzhouChina
  2. 2.Department of Plant PathologyKansas State UniversityManhattanUSA
  3. 3.USDA-ARS Hard Winter Wheat Genetics Research UnitManhattanUSA

Personalised recommendations