Chinese Science Bulletin

, Volume 57, Issue 5, pp 435–444

Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes

  • Dan Li
  • Wei Wang
  • Rui Guo
  • YiYing Qi
  • ZhongRu Gou
  • ChangYou Gao
Open Access
Article Progress of Projects Supported by NSFC Materials Science

DOI: 10.1007/s11434-011-4914-0

Cite this article as:
Li, D., Wang, W., Guo, R. et al. Chin. Sci. Bull. (2012) 57: 435. doi:10.1007/s11434-011-4914-0

Abstract

A composite construct comprising of a bone mesenchymal stem cell (BMSC) sheet, plasmid DNA, encoding human bone morphogenic protein-2 (hBMP-2), and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) sponge was designed and employed in the restoration of rat calvarial defects. To improve gene transfection efficiency, a cationic chitosan derivative, N,N,N,-trimethyl chitosan chloride (TMC), was employed as the vector. The TMC/DNA complexes had a transfection efficiency of 13% in rat BMSCs, resulting in heterogeneous hBMP-2 expression in a 10-d culture period in vitro. In vivo culture of the composite constructs was performed by implantation into rat full-thickness calvarial defects, using constructs lacking pDNA-hBMP-2 or BMSC sheets as controls. Significantly higher heterogeneous expression of hBMP-2 was detected in vivo at 2 weeks for the cell sheet/DNA complex/scaffold constructs, compared with the constructs lacking pDNA-hBMP-2 or BMSC sheets. New bone formation was evident as early as 4 weeks in the experimental constructs. At 8 weeks, partial bridging of calvarial defects was observed in the experimental constructs, which was significantly better than the constructs lacking pDNA-hBMP-2 or BMSC sheets. Therefore, the combination of the PLGA/HA scaffold with BMSC sheets and gene therapy vectors is effective at enhancing bone formation.

Keywords

gene therapy bone regeneration bone marrow stem cells poly(lactide-co-glycolide) BMP-2 

Copyright information

© The Author(s) 2011

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  • Dan Li
    • 1
    • 2
  • Wei Wang
    • 1
  • Rui Guo
    • 1
  • YiYing Qi
    • 3
  • ZhongRu Gou
    • 2
  • ChangYou Gao
    • 1
    • 4
  1. 1.Key Laboratory of Macromolecular Synthesis and Functionalization of Ministry of Education, Department of Polymer Science and EngineeringZhejiang UniversityHangzhouChina
  2. 2.Zhejiang-California International NanoSystems InstituteHangzhouChina
  3. 3.Department of Orthopedic Surgery, Second Affiliated HospitalZhejiang UniversityHangzhouChina
  4. 4.State Key Laboratory of Diagnosis and Treatment for Infectious Diseases, First Affiliated Hospital, College of MedicineZhejiang UniversityHangzhouChina

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