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Chinese Science Bulletin

, 56:3108 | Cite as

Ancient DNA sequences of rice from the low Yangtze reveal significant genotypic divergence

  • LongJiang FanEmail author
  • YiJie Gui
  • YunFei Zheng
  • Yu Wang
  • DaGuang Cai
  • XiuLing You
Open Access
Article Crop Genetics

Abstract

Rice (Oryza sativa) was first domesticated in the lower and middle Yangtze regions of China, and rice remains have been found in many Chinese archaeological sites. Until now, only phenotypic archeobotanical evidence, such as the spikelet bases of ancient grains, has been used to speculate on the domestication process and domestication rate of rice. In this study, we sequenced 4 genomic segments from rice remains in Tianluoshan, a site of the local Hemudu Neolithic culture in the low Yangtze and two other archaeological sites (∼2400 and 1200 BC, respectively). We compared our sequences with those of the current domesticated and wild rice (O. rufipogon) populations. At least two genotypes were found in the remains from each site, suggesting a heterozygotic state of the rice seeds. One ancient genotype was not found in the current domesticated population and might have been lost. The rice remains belonged to the japonica group, and most if not all were japonica-type, suggesting that the remains might be at an early stage of indica-japonica divergence or an indica-japonica mixture. We also identified sequences with significant similarity to those from species of Sapindales, Zygophyllales, and Brassicales, which is consistent with the identification of other plant remains in the Tianluoshan site and the common rice field weeds such as mustards in southern China.

Keywords

Oryza sativa ancient DNA sequence Hemudu Neolithic culture rice domestication 

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Copyright information

© The Author(s) 2011

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  • LongJiang Fan
    • 1
    Email author
  • YiJie Gui
    • 1
  • YunFei Zheng
    • 2
  • Yu Wang
    • 1
  • DaGuang Cai
    • 3
  • XiuLing You
    • 4
  1. 1.Department of AgronomyZhejiang UniversityHangzhouChina
  2. 2.Zhejiang Provincial Institute of Archaeology and Cultural RelicsHangzhouChina
  3. 3.Department of Molekulare PhytopathologieChristian-Albrechts-Universität zu KielKielGermany
  4. 4.School of HumanitiesZhejiang UniversityHangzhouChina

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