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Chinese Science Bulletin

, Volume 56, Issue 14, pp 1476–1480 | Cite as

Genetic analysis and fine mapping of an enclosed panicle mutant esp2 in rice (Oryza sativa L.)

  • HuaZhong Guan
  • YuanLin Duan
  • HuaQing Liu
  • ZhiWei Chen
  • Ming Zhuo
  • LiJun Zhuang
  • WenMing Qi
  • RunSen Pan
  • DaMei Mao
  • YuanChang Zhou
  • Feng WangEmail author
  • WeiRen WuEmail author
Open Access
Article Crop Genetics

Abstract

The phenomenon of panicle enclosure in rice is mainly caused by the shortening of uppermost internode. Elucidating the molecular mechanism of panicle enclosure will be helpful for solving the problem of panicle enclosure in male sterile lines and creating new germplasms in rice. We acquired a monogenic recessive enclosed panicle mutant, named as esp2 (enclosed shorter panicle 2), from the tissue culture progeny of indica rice cultivar Minghui-86. In the mutant, panicles were entirely enclosed by flag leaf sheaths and the uppermost internode was almost completely degenerated, but the other internodes did not have obvious changes in length. Genetic analysis indicated that the mutant phenotype was controlled by a recessive gene, which could be steadily inherited and was not affected by genetic background. Apparently, ESP2 is a key gene for the development of uppermost internode in rice. Using an F2 population of a cross between esp2 and a japonica rice cultivar Xiushui-13 as well as SSR and InDel markers, we fine mapped ESP2 to a 14-kb region on the end of the short arm of chromosome 1. According to the rice genome sequence annotation, only one intact gene exists in this region, namely, a putative phosphatidylserine synthase gene. Sequencing analysis on the mutant and the wild type indicated that this gene was inserted by a 5287-bp retrotransposon sequence. Hence, we took this gene as a candidate of ESP2. The results of this study will facilitate the cloning and functional analysis of ESP2 gene.

Keywords

rice enclosed panicle mutant ESP2 gene fine mapping 

References

  1. 1.
    Wu C, Deng X J, Liu A Q, et al. Strategy of gene engineering in hybrid rice breeding in China (in Chinese). Biotechnol Bull, 2003, (1): 4–7Google Scholar
  2. 2.
    Deng H F, He Q, Shu F, et al. Status and technical strategy on development of japonica hybrid rice in China (in Chinese). Hybrid Rice, 2006, 21: 1–6Google Scholar
  3. 3.
    Li A X, Li Z H, Ding K X, et al. Measures for preventing and controlling kernel Smut in hybrid rice seed production (in Chinese). Jiangsu Agric Sci, 1995, 4: 34–36Google Scholar
  4. 4.
    Rutger J N, Camahan H L. A fourth genetic element to facilitate hybrid cereal productions recessive tall in rice. Crop Sci, 1981, 21: 373–376CrossRefGoogle Scholar
  5. 5.
    Yang R C, Zhang S B, Huang R H, et al. Breeding technology of eui-hybrids of rice. Agr Sci Sin, 2002, 35: 233–237Google Scholar
  6. 6.
    He Z H, Li D B. Relations of plant height genes to the sensitivity of GA3 and to the regulation of endogenous in different rice growth stages. Plant Physiol Commun, 1994, 30: 170–174Google Scholar
  7. 7.
    Zhu H B. Fine mapping and cloning of rice EUI2(t) gene controlling upper most internode elongation. Doctor Dissertation. Fu Zhou: Fujian Agriculture and Forestry University, 2003Google Scholar
  8. 8.
    Zhu Y Y, Nomura T, Xu Y H, et al. Elongated uppermost internode encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice. Plant Cell, 2006, 18: 442–456CrossRefGoogle Scholar
  9. 9.
    He Z H, Shen Z T. Sensitivity of elongated internode gene to GA3 and improvement of ms line in rice (in Chinese). Acta Agron Sin, 1994, 20: 161–167Google Scholar
  10. 10.
    Liang K J, Wang N Y, Yang R C. Inheritance and breeding utilization of panicle exsertion of rice (in Chinese). J Fujian Agric Univ (Nat Sci), 1992, 21: 380–385Google Scholar
  11. 11.
    Shen Z T, Yang C D, He Z H. Studies on eliminating panicle enclosure in WAType MS line of rice (Oryza sativa subsp. indica)(in Chinese). J Chi Rice Sic, 1987, 1: 95–99Google Scholar
  12. 12.
    Virmani S S, Dalmacio R D, Lopez M T. EUI gene for elongated uppermost internode transferred to indica rice. Int Rice Res Newsl, 1988, 13: 6–9Google Scholar
  13. 13.
    Zhu K M. Genetic analysis and mapping of SHP6 gene in rice (in Chinese). Master Dissertation. Yangzhou: Yangzhou University, 2006Google Scholar
  14. 14.
    Kinoshita T. Report of the committee on gene symbolization nomenclature and linkage groups. Rice Genet News, 1990, 7: 16–50Google Scholar
  15. 15.
    Liu Z, Luo L J. Anatomical studies on the stem of rice of dwarf and sheathed panicle (in Chinese). Chin Agri Sci Bull, 2006, 22: 409–412Google Scholar
  16. 16.
    Wang W P, Zhu F Z, Tang L, et al. Discovery and preliminary analysis of a rice mutant with fully sheathed panicle (in Chinese). Chin Agri Sci Bull, 2008, 24: 212–216Google Scholar
  17. 17.
    Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832CrossRefGoogle Scholar
  18. 18.
    Lander E S, Green P, Abrahamson J, et al. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174–181CrossRefGoogle Scholar
  19. 19.
    Duan Y L, Wu W R, Liu H Q, et al. Genetic analysis and gene mapping of leafy head (lhd), a mutant blocking the differentiation of rachis branches in rice (Oryza sativa L.) (in Chinese). Chinese Sci Bull, 2003, 48: 2201–2205Google Scholar
  20. 20.
    Kidd P. Phosphatidylserine: Membrane nutrient for memory. A clinical and mechanistic assessment. Altern Med Rev, 1996, 1: 70–84wGoogle Scholar
  21. 21.
    Leiros I, McSweeney S, Hough E. The reaction mechanism of phospholipase D from Streptomyces sp. Strain PMF. Snapshots along the reaction pathway reveal a pentacoordinate reaction intermediate and an unexpected final product. Mol Biol, 2004, 339: 805–820CrossRefGoogle Scholar

Copyright information

© The Author(s) 2011

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited.

Open AccessThis is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  • HuaZhong Guan
    • 1
    • 2
  • YuanLin Duan
    • 1
    • 2
  • HuaQing Liu
    • 3
  • ZhiWei Chen
    • 1
    • 2
  • Ming Zhuo
    • 1
  • LiJun Zhuang
    • 1
  • WenMing Qi
    • 1
  • RunSen Pan
    • 1
    • 2
  • DaMei Mao
    • 1
    • 2
  • YuanChang Zhou
    • 1
    • 2
  • Feng Wang
    • 3
    Email author
  • WeiRen Wu
    • 1
    • 2
    Email author
  1. 1.Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of CropsFujian Agricultural & Forestry UniversityFuzhouChina
  2. 2.Fujian Provincial Key Laboratory of Marker-Assisted Breeding of RiceFujian Agriculture and Forestry UniversityFuzhouChina
  3. 3.Fujian Agricultural Science Research InstituteFuzhouChina

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