Chinese Science Bulletin

, Volume 55, Issue 12, pp 1127–1134 | Cite as

Stable expression of Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1, and salt tolerance in transgenic soybean for over six generations

  • TianXing (YiZhou) Li
  • Yue Zhang
  • Hua Liu
  • YuTing Wu
  • WenBin Li
  • HongXia Zhang
Articles Gene Engineering
First Online:
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Accepted:

Abstract

The Arabidopsis vacuolar Na+/H+ antiporter gene, AtNHX1, was introduced into soybean by Agrobacterium-mediated transformation. Four independent kanamycin resistant lines were obtained. The result of PCR, Southern blotting and Northern blotting analyses demonstrated that the AtNHX1 gene was successfully inserted into the soybean genome and stably expressed in these kanamycin resistant lines. The stability of AtNHX1 expression and salt resistance were evaluated in the soybean transformants for over 6 generations. Two independently derived transgenic lines with high expression level of AtNHX1 were selected, and propagated to generation T5 in the absence of selection pressure. PCR and RT-PCR examinations revealed that AtNHX1 was highly expressed in all investigated transgenic T5 progenies. Furthermore, all transgenic T5 plants showed resistant to salt stress, same as those of homozygous T2 plants. Taken together, our results indicated that constitutive expression of AtNHX1 enhanced salt tolerance in soybean for over 6 generations, suggesting a great potential use of AtNHX1 for improving salt tolerance in plants by genetic engineering.

Keywords

AtNHX1 fatty acids salt tolerance soybean transgenic plants 
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Copyright information

© Science in China Press and Springer Berlin Heidelberg 2010

Authors and Affiliations

  • TianXing (YiZhou) Li
    • 1
    • 2
  • Yue Zhang
    • 2
  • Hua Liu
    • 2
  • YuTing Wu
    • 2
  • WenBin Li
    • 3
  • HongXia Zhang
    • 2
  1. 1.College of Life SciencesYunnan UniversityKunmingChina
  2. 2.National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina
  3. 3.Key Laboratory of Soybean Biology in Chinese Education MinistryNortheast Agricultural UniversityHarbinChina

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