Chinese Science Bulletin

, Volume 54, Issue 15, pp 2648–2655 | Cite as

Phylogenetic relationships and divergence times of the family Araucariaceae based on the DNA sequences of eight genes

  • Nian Liu
  • Yong Zhu
  • ZongXian Wei
  • Jie Chen
  • QingBiao Wang
  • ShuGuang Jian
  • DangWei Zhou
  • Jing Shi
  • Yong Yang
  • Yang Zhong
Articles/Molecular Revolution

Abstract

Araucariaceae is one of the most primitive families of the living conifers, and its phylogenetic relationships and divergence times are critically important issues. The DNA sequences of 8 genes, i.e., nuclear ribosomal 18S and 26S rRNA, chloroplast 16S rRNA, rbcL, matK and rps4, and mitochondrial coxI and atp1, obtained from this study and GenBank were used for constructing the molecular phylogenetic trees of Araucariaceae, indicating that the phylogenetic relationships among the three genera of this family should be ((Wollemia, Agathis), Araucaria). On the basis of the fossil calibrations of Wollemia and the two tribes Araucaria and Eutacta of the genus Araucaria, the divergence time of Araucariaceae was estimated to be (308 ± 53) million years ago, that is, the origin of the family was in the Late Carboniferous rather than Triassic as a traditional view. With the same gene combination, the divergence times of the genera Araucaria and Agathis were (246 ± 47) and (61 ± 15) Ma, respectively. Statistical analyses on the phylogenetic trees generated by using different genes and comparisons of the divergence times estimated by using those genes suggested that the chloroplast matK and rps4 genes are most suitable for investigating the phylogenetic relationships and divergence times of the family Araucariaceae.

Keywords

Araucariaceae nrDNA 18S rRNA nrDNA 26S rRNA cpDNA 16S rRNA cpDNA rbccpDNA matcpDNA rpsmtDNA coxmtDNA atpphylogeny divergence time 

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Copyright information

© Science in China Press and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Nian Liu
    • 1
  • Yong Zhu
    • 1
  • ZongXian Wei
    • 2
  • Jie Chen
    • 1
  • QingBiao Wang
    • 1
  • ShuGuang Jian
    • 4
  • DangWei Zhou
    • 5
  • Jing Shi
    • 1
  • Yong Yang
    • 6
  • Yang Zhong
    • 1
    • 3
  1. 1.Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life SciencesFudan UniversityShanghaiChina
  2. 2.Lushan Botanical GardenChinese Academy of SciencesLushanChina
  3. 3.Shanghai Center for Bioinformation TechnologyShanghaiChina
  4. 4.South China Botanical GardenChinese Academy of SciencesGuangzhouChina
  5. 5.Northwest Institute of Plateau BiologyChinese Academy of SciencesXiningChina
  6. 6.Laboratory of Systematic and Evolutionary Botany, Institute of BotanyChinese Academy of SciencesBeijingChina

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