Chinese Science Bulletin

, 53:3559 | Cite as

Geographical genetic diversity and divergence of common wild rice (O. rufipogon Griff.) in China

  • MeiXing Wang
  • HongLiang Zhang
  • DongLing Zhang
  • DaJian Pan
  • DaoYuan Li
  • ZhiLan Fan
  • YongWen Qi
  • JunLi Sun
  • QingWen Yang
  • Chen Li
  • ZiChao Li
Articles/Crop Germplasm Resources

Abstract

Using 36 SSR markers and 889 accessions of common wild rice in China, the genetic diversity and the divergence among different geographical populations are investigated. Guangdong Province has the largest number of alleles, which account for 84% of the total alleles detected in the study, followed by Guangxi Province. The Nei’s gene diversity indices, from high to low, are in the sequence of Hainan, Guangdong, Guangxi, Fujian, Hunan, Jiangxi, and Yunnan provinces. Two genetic diversity centers of Chinese common wild rice are detected on the basis of geographic analysis, i.e., the region covering Boluo, Zijin, Lufeng, Haifeng, Huidong and Huiyang counties of Guangdong Province and the region covering Yongning, Longan, Laibin and Guigang counties of Guangxi Province. The common wild rice in Yunnan, Hunan, Jiangxi, and Fujian provinces are diverged into respectively independent populations with relatively large genetic distances, whereas, those in Hainan, Guangdong and Guangxi provinces have relatively low genetic divergence. Under the condition of geographic separation, natural selection is considered as one of the primary forces contributing to the divergence of common wild rice in China.

Keywords

common wild rice genetic diversity genetic difference SSR 

References

  1. 1.
    Oka H I. Experimental studies on the origin of cultivated rice. Genetics, 1974, 78: 475–486PubMedGoogle Scholar
  2. 2.
    Yuan L P, Virmani S S, Mao C X. Hybrid rice: Achievements and further outlook. In: Wilson K J, ed. Progress in Irrigated Rice Research. Manila: International Rice Research Institute, 1989. 219–223Google Scholar
  3. 3.
    Xiao J H, Grandillo S, Ahn S N, et al. Genes from wild rice improve yield. Nature, 1996, 384: 223–224CrossRefGoogle Scholar
  4. 4.
    Second G. Origin of the genetic diversity of cultivated rice (Oryza spp.): Study of the polymorphism scored at 40 isozyme loci. Jpn J Genet, 1982, 57: 25–57CrossRefGoogle Scholar
  5. 5.
    Yu P, Li Z C, Zhang H L, et al. Genetic diversity of common wild rice (Oryza rufipogon Griff) by using SSR markers and phenotypic traits in Guangxi Province (in Chinese). Acta Genet Sin, 2004, 31(9): 934–940PubMedGoogle Scholar
  6. 6.
    Wang X N, Han D F, Yun Y, et al. Genetic diversity of Oryza rufipogon Griff. in Hainan Province with SSR markers (in Chinese). J Plant Genet Resour, 2007, 8(2): 184–188Google Scholar
  7. 7.
    Li G H, Li G X, Huang Y J, et al. Study on genetic diversity of dongxiang wild rice (Oryza rufipogon Griff.)-Allozyme (in Chinese). Acta Agronom Sin, 2004, 30(9): 927–931Google Scholar
  8. 8.
    Yang Q W, Dai L Y, Shi J X, et al. Study of genetic diversity and conservation strategy of (Oryza rufipogon Griff) in Yuanjiang (in Chinese). J Plant Genet Resour, 2004, 5(1): 1–5Google Scholar
  9. 9.
    Gao L Z, Hong D Y, Ge S. Allozyme variation and population genetic structure of common wild rice (Oryza rufipogon Griff.) in China. Theor Appl Genet, 2000, 101: 494–502CrossRefGoogle Scholar
  10. 10.
    Gao L Z, Chen W, Jiang W Z, et al. Genetic erosion in the Northern marginal population of the common wild rice (Oryza rufipogon Griff.), and its conservation, revealed by the change of population genetic structure. Hereditas, 2000, 133: 47–53PubMedCrossRefGoogle Scholar
  11. 11.
    Song Z P, Xu X, Wang B, et al. Genetic diversity in the northernmost Oryza rufipogon populations estimated by SSR markers. Theor Appl Genet, 2003, 107: 1492–1499PubMedCrossRefGoogle Scholar
  12. 12.
    Zhou H F, Xie Z W, Ge S. Microsatellite analysis of genetic diversity and population genetic structure of a wild rice (Oryza rufipogon Griff.) in China. Theor Appl Genet, 2003, 107:332–339PubMedCrossRefGoogle Scholar
  13. 13.
    Wang Y H, Wang H, Gao L Z. Study on genetic diversity of Oryza rufipogon Griff. by simple seqence repeat. Acta Bot Boreali-Occidentalia Sin, 2003, 23(10): 1750–1754Google Scholar
  14. 14.
    Yu P, Li Z C, Zhang H L, et al. Sampling strategy of primary core collection of common wild rice (Oryza rufipogon Griff.) in China (in Chinese). J China Agricul Univ, 2003, 8(5): 37–41Google Scholar
  15. 15.
    Rogers O S, Bendich A J. Extraction of DNA from plant tissues. In: Gelvin S B, Schiliperoort R A, Verma D P S, eds. Plant Molecular Biology Manual. Boston: Kluwer Academic Pulblishers, 1988. A6: 1–10Google Scholar
  16. 16.
    Bassam B J, Caetano-Anolles G, Gresshoff P M. Fast and sensitive silver staining of DNA in polyacrylamine gels. Anal Biochem, 1991, 196: 80–83PubMedCrossRefGoogle Scholar
  17. 17.
    Liu K, Muse S. PowerMarker: New Genetic Data Analysis Software, 2004, Version 2.7 (http://www.powermarker.net)
  18. 18.
    Kalinowski S T. HP-RARE1.0: A computer program for performing rarefaction on measures of allelic richness. Mol Ecol Notes, 2005, 5: 187–189CrossRefGoogle Scholar
  19. 19.
    Rohlf F J. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Version 2.00. New York: Exeter Software, 1997Google Scholar
  20. 20.
    Nei M, Tajima F, Tateno Y. Accuracy of estimated phylcgenetic trees from molecular data. J Mol Evol, 1983, 19: 153–170PubMedCrossRefGoogle Scholar
  21. 21.
    Wang X K, Sun C Q, Cai H W, et al. Studies on Classification and Genetic Diversity of Common Wild Rice (Oryza rufipogon Griff.) in Asia. In: Yang Q W, Chen D Z, eds. Studies and Applications of Wild Rice in China: Proceedings of the First National Conference on Wild Rice in China (in Chinese). Beijing: China Meteorological Press, 2004. 107–117Google Scholar
  22. 22.
    Gao L Z, Schaal B A, Zhang C h, et al. Assessment of population genetic structure in common wild rice (Oryza rufipogon Griff.) using microsatellite and allozyme markers. Theor Appl Genet, 2002, 106: 173–180PubMedGoogle Scholar
  23. 23.
    Sun C Q, Wang X K, Li Z C, etal. Comparison of the genetic diversity of common wild rice (Oryza rufipogon Griff.) and cultivated rice (O. sativa L.) using RFLP markers. Theor Appl Genet, 2001, 102: 157–162CrossRefGoogle Scholar
  24. 24.
    Zhang X L, Guo H, Wang H G. Comparative assessment of SSR allelic diversity in wild and cultivated rice in China (in Chinese). Acta Agronom Sin, 2008, 34(4): 591–597CrossRefGoogle Scholar
  25. 25.
    Allard R W, Zhang Q F, Saghai Maroof M A, et al. Evolution of multilocus genetic structure in an experimental barley population. Genetics, 1992, 131: 957–969PubMedGoogle Scholar
  26. 26.
    Soltis P S, Soltis D E. Genetic variation in endemic and widespread plant species: Examples from Saxifragageae and Polystichum (Dryopteridaceae). Aliso, 1991, 13: 215–223Google Scholar
  27. 27.
    Dong Y S, Zhuang B C, Zhao L M, et al. The genetic diversity of annual wild soybeans grown in China. Theor Appl Genet, 2001, 103: 98–103CrossRefGoogle Scholar
  28. 28.
    Cai H W, Wang X K, Morishima H. Comparison of population genetic structures of common wild rice (Oryza rufipogon Griff.), as revealed by analyses of quantitative traits, allozymes and RFLPs. Heredity, 2004, 92: 409–417PubMedCrossRefGoogle Scholar

Copyright information

© Science in China Press and Springer-Verlag GmbH 2008

Authors and Affiliations

  • MeiXing Wang
    • 1
    • 5
  • HongLiang Zhang
    • 1
  • DongLing Zhang
    • 1
  • DaJian Pan
    • 2
  • DaoYuan Li
    • 3
  • ZhiLan Fan
    • 2
  • YongWen Qi
    • 1
  • JunLi Sun
    • 1
  • QingWen Yang
    • 4
  • Chen Li
    • 2
  • ZiChao Li
    • 1
  1. 1.Key Laboratory of Crop Genomics & Genetic Improvement of Ministry of Agriculture and Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijingChina
  2. 2.Rice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhouChina
  3. 3.Rice Research InstituteGuangxi Academy of Agricultural SciencesNanningChina
  4. 4.Institute of Crop ScienceCAASBeijingChina
  5. 5.Institute of Crops and Utilization of Nuclear TechnologyZhejiang Academy of Agricultural SciencesHangzhouChina

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