Chinese Science Bulletin

, Volume 53, Issue 23, pp 3666–3676 | Cite as

Analysis of genes differentially expressed during initial cellular dedifferentiation in cotton

  • HuaGuo Zhu
  • LiLi Tu
  • ShuangXia Jin
  • Li Xu
  • JiaFu Tan
  • FengLin Deng
  • XianLong Zhang
Articles/Plant Developmental Biology

Abstract

The early phase of phytohormone induction is a vital stage of somatic embryogenesis. This phase includes a key process for acquiring cellular totipotency through cellular dedifferentiation. To unravel the molecular mechanism of cellular dedifferentiation in cotton, we constructed a cDNA library using the suppression subtractive hybridization method. A total of 286 differential cDNA clones were sequenced and identified. Among these clones, 112 unique ESTs were significantly up-regulated during the early phase of phytohormone induction, and 40.2% of the ESTs were first identified. GST was highly expressed from 6 to 24 h after induction with phytohormone treatment. PRPs were predominantly expressed and exhibited distinct expression patterns in different treatments, suggesting that they are closely related to cellular dedifferentiation in cotton. Putative GhSAMS, GhSAMDC, GhSAHH and GhACO3 involvement in SAM metabolism was identified in this library. The analysis of qRT-PCR showed that two remarkable increased expressions of the four SAM-related genes happened during the early phase of phytohormone induction, and that a highly positive correlation existed between GhSAMS and GhSAHH. The highest expression level of GhSAMS might be associated with its reentry into the cell cycle. The histological observations further showed that some cells accomplished cellular dedifferentiation and division within 72 h in 2,4-D treatment, and that cellular dedifferentiation might be regulated through two alterations in SAM-dependent transmethylation activity in cotton. In addition, the expression patterns of differential genes in different treatments disclosed the complicated interaction between 2, 4-D and kinetin.

Keywords

cellular dedifferentiation cotton proline-rich protein SAM-dependent transmethylation suppression subtractive hybridization 

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Copyright information

© Science in China Press and Springer-Verlag GmbH 2008

Authors and Affiliations

  • HuaGuo Zhu
    • 1
  • LiLi Tu
    • 1
  • ShuangXia Jin
    • 1
  • Li Xu
    • 1
  • JiaFu Tan
    • 1
  • FengLin Deng
    • 1
  • XianLong Zhang
    • 1
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina

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