Chinese Science Bulletin

, Volume 53, Issue 2, pp 198–203

Study on the specific interaction between angiogenin and aptamer by atomic force microscopy (AFM)

  • He XiaoXiao 
  • Jin Rong 
  • Yang Liu 
  • Wang KeMin 
  • Li Wei 
  • Tan WeiHong 
  • Li HuiMin 
Articles Analytical Chemistry

Abstract

The specific interaction between angiogenin and aptamer has been investigated by using AFM. The specificity of the interaction is revealed by comparing the binding probability of aptamer to other elements in a series of control experiments. The results have shown that there is specific interaction force between angiogenin and aptamer. Moreover, the single molecular pull-off force between angiogenin and aptamer has also been determined using the Poisson statistical method to be 133.7±11.7 pN. These findings obtained are helpful to the better revelation of recognition mechanism between angiogenin and aptamer, which provided basis for further understanding the inhibition of the aptamer to angiogenic activity.

Keywords

atomic force microscopy (AFM) angiogenin aptamer force detection 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Fett J W, Strydom D J, Lobb R R, et al. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells. Biochem, 1985, 24(20): 5480–5486CrossRefGoogle Scholar
  2. 2.
    Shapiro R, Strydom D J, Olson K A, et al. Isolation of angiogenin from normal human plasma. Biochem, 1987, 26(16): 5141–5146CrossRefGoogle Scholar
  3. 3.
    Bai D Y, Ma H D. The role of angiopoietin in angiogenesis. Acta Academiae Medicinae CPAPF (in Chinese), 2005, 14(4): 323–325Google Scholar
  4. 4.
    Ahmad S A, Liu W, Jung Y D, et al. The effects of angiopoietin-1 and -2 on tumor growth and angiogenesis in human colon cancer. Cancer Res, 2001, 61(4): 1255–1259PubMedGoogle Scholar
  5. 5.
    Eggert A, Ikegaki N, Kwiatkowski J, et al. High-level expression of angiogenic factors is associated with advanced tumor stage in human neuroblastomas. Clin Cancer Res, 2000, 6(5): 1900–1908PubMedGoogle Scholar
  6. 6.
    Papapetropoulos A, Fulton D, Mahboubi K, et al. Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/ survivin pathway. J Biol Chem, 2000, 275(13): 9102–9105PubMedCrossRefGoogle Scholar
  7. 7.
    Wurmbach J H, Hammerer P, Sevine S, et al. The expression of angiopoietins and their receptor Tie-2 in human prostate carcinoma. Anticancer Res, 2000, 20: 5217–5220PubMedGoogle Scholar
  8. 8.
    Gao D L, Guo K M, Seng J J, et al. Expression of angtopoietin-2 and Tie-2 in gastric carcinoma tissues. Journal of Medical Forum (in Chinese), 2005, 26(20): 40–41Google Scholar
  9. 9.
    Nobile V, Russo N, Hu G F, et al. Inhibition of human angiogenin by DNA aptamers: nuclear colocalization of an angiogenin-inhibitor complex. Biochem. 1998, 37(19): 6857–6863CrossRefGoogle Scholar
  10. 10.
    Li Y X, He F C. The methods for studies of interaction between DNA and proteins. Chemistry of Life (in Chinese), 2003, 23(4): 306–308Google Scholar
  11. 11.
    Yang L, Wang K M, Tan W H, et al. Atomic force microscopy study of different effects of natural and semi-synthetic beta-lactam on the cell envelope of Escherichia coli. Anal Chem. 2006, 78: 7341–7345PubMedCrossRefGoogle Scholar
  12. 12.
    Yamashina S, Shigeno M. Application of atomic force microscopy to ultrastructural and histochemical studied of fixed and embedded cells. J Electron Microsc, 1995, 44(6): 462–466Google Scholar
  13. 13.
    Mingorance J, Tadros M, Vicente M, et al. Visualization of single Escherichia coli FtsZ filament dynamics with atomic force microscopy. J Biol Chem, 2005, 280(21): 20909–20914PubMedCrossRefGoogle Scholar
  14. 14.
    Wang Z G, Wan L J, Zhou C Q, et al. Study of â-amyloid adsorption and aggregation on graphite by STM and AFM. Chin Sci Bull, 2003, 48(5): 437–440CrossRefGoogle Scholar
  15. 15.
    Qin F, Jiang Y X, Ma X Y, et al. A study of specific interaction of the transcription factor and the DNA element by atomic force microscopy. Chin Sci Bull, 2004, 49(13): 1376–1380CrossRefGoogle Scholar
  16. 16.
    Allen S, Chen X Y, Davies J, et al. Detection of antigen-antibody binding envents with the atomic force microscope. Biochem, 1997, 36(24): 7457–7463CrossRefGoogle Scholar
  17. 17.
    Yuan C, Chen A, Kolb P, et al. Energy landscape of streptavidin-biotin complexes measured by atomic force microscopy. Biochem, 2000, 39(33): 10219–10223CrossRefGoogle Scholar
  18. 18.
    Ros R, Schwesinger F, Anselmetti D, et al. Antigen binding forces of individually addressed single-chain Fv antibody molecules. Proc Natl Acad Sci USA, 1998, 95(13): 7402–7405PubMedCrossRefGoogle Scholar
  19. 19.
    Lo Y S, Huefner N D, Chan W S, et al. Specific Interactions between biotin and avidin studied by atomic force microscopy using the Poisson statistical analysis method. Langmuir, 1999, 15(4): 1373–1382CrossRefGoogle Scholar
  20. 20.
    Wong J, Chilkoti A, Moy V T. Direct force measurements of the streptavidin-biotin interaction. Biomolecular Engineering, 1999, 16(1–4): 45–55PubMedCrossRefGoogle Scholar
  21. 21.
    Jiang Y X, Zhu C F, Ling L S, et al. Specific Aptamer-Protein Interaction Studied by Atomic Force Microscopy. Anal Chem, 2003, 75(9): 2112–2116PubMedCrossRefGoogle Scholar
  22. 22.
    Basnar B, Elnathan R, Willner I. Following aptamer-thrombin binding by force measurements. Anal Chem, 2006, 78(11): 3638–3642PubMedCrossRefGoogle Scholar
  23. 23.
    Jin Y, Wang K M, Tan W H, et al. Monitoring molecular beacon/DNA interactions using atomic force microscopy. Anal Chem, 2004, 76(19): 5721–5725PubMedCrossRefGoogle Scholar
  24. 24.
    Lo Y S, Simons J, Beebe T P. Loading-rate dependence of individual ligand-receptor bond-rupture forces studied by atomic force microscopy. Langmuir, 2001, 17(12): 3741–3748CrossRefGoogle Scholar
  25. 25.
    Williams J M, Han T, Beebe T P J. Determination of single-bond forces from contact force variances in atomic force microscopy. Langmuir, 1996, 12(5): 1291–1295CrossRefGoogle Scholar
  26. 26.
    Stevens F, Lo Y S, Harris J M, et al. Computer modeling of atomic force microscopy force measurements: comparisons of Poisson, Histogram, and Continuum methods. Langmuir, 1999, 15(1): 207–213CrossRefGoogle Scholar
  27. 27.
    Wenzler L A, Moyes G L, Olson L G, et al. Single-molecule bond-rupture force analysis of interactions between AFM tips and substrates modified with organosilanes. Anal Chem, 1997, 69(14): 2855–2861CrossRefGoogle Scholar
  28. 28.
    Tian S X, Cui X Y. The investigation of human angiogenin. Chemistry of Life (in Chinese), 1996, 16(3): 25–26Google Scholar

Copyright information

© Science in China Press 2008

Authors and Affiliations

  • He XiaoXiao 
    • 1
  • Jin Rong 
    • 1
  • Yang Liu 
    • 1
  • Wang KeMin 
    • 1
  • Li Wei 
    • 1
  • Tan WeiHong 
    • 1
  • Li HuiMin 
    • 1
  1. 1.State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Life Science and Biological Technology, Engineering Center for Biomedicine, College of Material Science and TechnologyHunan University, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan ProvinceChangshaChina

Personalised recommendations