Chinese Science Bulletin

, Volume 53, Issue 2, pp 198–203

Study on the specific interaction between angiogenin and aptamer by atomic force microscopy (AFM)

  • He XiaoXiao 
  • Jin Rong 
  • Yang Liu 
  • Wang KeMin 
  • Li Wei 
  • Tan WeiHong 
  • Li HuiMin 
Articles Analytical Chemistry


The specific interaction between angiogenin and aptamer has been investigated by using AFM. The specificity of the interaction is revealed by comparing the binding probability of aptamer to other elements in a series of control experiments. The results have shown that there is specific interaction force between angiogenin and aptamer. Moreover, the single molecular pull-off force between angiogenin and aptamer has also been determined using the Poisson statistical method to be 133.7±11.7 pN. These findings obtained are helpful to the better revelation of recognition mechanism between angiogenin and aptamer, which provided basis for further understanding the inhibition of the aptamer to angiogenic activity.


atomic force microscopy (AFM) angiogenin aptamer force detection 


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Copyright information

© Science in China Press 2008

Authors and Affiliations

  • He XiaoXiao 
    • 1
  • Jin Rong 
    • 1
  • Yang Liu 
    • 1
  • Wang KeMin 
    • 1
  • Li Wei 
    • 1
  • Tan WeiHong 
    • 1
  • Li HuiMin 
    • 1
  1. 1.State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Life Science and Biological Technology, Engineering Center for Biomedicine, College of Material Science and TechnologyHunan University, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan ProvinceChangshaChina

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