Chinese Science Bulletin

, Volume 52, Issue 18, pp 2522–2531 | Cite as

Studies of new EST-SSRs derived from Gossypium barbadense

  • Zhang YanXin 
  • Lin ZhongXu 
  • Li Wu 
  • Tu LiLi 
  • Nie YiChun 
  • Zhang XianLong 
Articles Geneticss

Abstract

Existing cotton EST-SSR markers are mostly derived from Gossypium arboreum and Gossypium hirsutum, but EST-SSR markers from Gossypium barbadense are scarce. One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G. barbadense cv. Pima3–79. Among the SSRs, trinucleotide AAG appeared at a high frequency of 11.76%. 36 accessions (consisting of 13 diploids of the A genome, 11 diploids of the D genome and 12 allotetraploids of the AD genome) were employed to test new EST-SSRs. 76 EST-SSRs were successfully amplified, and 313 polymorphic fragments were yielded, with an average of 4.11 fragments per primer pair. The PIC ranged from 0.17 to 0.95 with an average of 0.53. Based on Jaccard’s genetic similarity coefficient, these 36 accessions were clustered into three groups. 21 EST-SSRs exhibited polymorphisms in BC1 population ((Emian22 × Pima3–79) × Emian22), 24 polymorphic loci were generated, while 22 of the 24 polymorphic loci were integrated with our interspecific BC1 backbone genetic linkage map, and anchored in 12 chromosomes. This study effectively proved that EST-SSRs from G. barbadense are valuable for genetic diversity analysis and genetic mapping.

Keywords

Gossypium barbadense expressed sequence tag (EST) simple sequence repeat (SSR) genetic diversity genetic linkage map 

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Copyright information

© Science in China Press 2007

Authors and Affiliations

  • Zhang YanXin 
    • 1
  • Lin ZhongXu 
    • 1
  • Li Wu 
    • 1
  • Tu LiLi 
    • 1
  • Nie YiChun 
    • 1
  • Zhang XianLong 
    • 1
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina

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