Chinese Science Bulletin

, Volume 52, Issue 4, pp 477–483 | Cite as

Detection of quantitative trait loci and heterotic loci for plant height using an immortalized F2 population in maize

  • Tang JiHua 
  • Ma XiQing 
  • Teng WenTao 
  • Yan JianBing 
  • Wu WeiRen 
  • Dai JingRui 
  • Li JianSheng 


A set of recombinant inbred lines (RIL) derived from Yuyu22, an elite hybrid widespread in China, was used to construct an immortalized F2 (IF2) population comprising 441 different crosses. Genetic linkage maps were constructed containing 10 linkages groups with 263 simple sequence repeat (SSR) molecular markers. Twelve and ten quantitative trait loci (QTL) were detected for plant height in the IF2 and RIL populations respectively, using the composite interval mapping method, and six same QTL were identified in the two populations. In addition, ten unique heterotic loci (HL) located on seven different chromosomes were revealed for plant height using the mid-parent heterosis as the input data. These HL explained 1.26%–8.41% of the genotypic variance in plant height heterosis and most expressed overdominant effects. Only three QTL and HL were located in the same chromosomal region, it implied that plant height and its heterosis might be controlled by two types of genetic mechanisms.


maize plant height heterotic loci QTL detection 


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  1. 1.
    Duvick D N. Heterosis: Feeding people and protecting natural resources. In: Coors J G, Pandey S, eds. Genetics and Exploitation of Heterosis in Crops. Wisconsin: Am Soc Agron, Crop Sci Soc Am, Soil Sci Soc Am, Inc, 1999. 19–29Google Scholar
  2. 2.
    Bruce A B. The Mendelian theory of heredity and the augmentation of vigor. Science, 1910, 32: 627–628CrossRefGoogle Scholar
  3. 3.
    Davenport C B. Degeneration albinism and inbreeding. Science, 1908, 28: 454–455.CrossRefGoogle Scholar
  4. 4.
    East E M. Heterosis. Genetics, 1936, 21: 375–397Google Scholar
  5. 5.
    Keeble F, Pellew C. The mode of inheritance of stature and of time of flowering in peas (Pisum sativum). Genetics, 1910, 1: 47–56CrossRefGoogle Scholar
  6. 6.
    Jones D F. Dominance of linked factors as a means of accounting for heterosis. Genetics, 1917, 2: 466–479Google Scholar
  7. 7.
    Shull G H. The composition of a field of maize. Am Breeders Assoc Rep, 1908, 4: 196–301Google Scholar
  8. 8.
    Lu H, Romero-Severson J, Bernarbo R. Genetic basis of heterosis explored by simple sequence repeat markers in a random-mated maize population. Theor Appl Genet, 2003, 107: 494–502CrossRefGoogle Scholar
  9. 9.
    Stuber C W, Lincoln S E, Wolff H T, et al. Identification of genetic factors contributing to heterosis in a hybrid from elite maize inbred lines using molecular markers. Genetics, 1992, 132(11): 823–839Google Scholar
  10. 10.
    Xiao J H, Li J M, Yuan L P, et al. Dominance is the major genetic basis of the heterosis in rice as revealed by QTL analysis using molecular markers. Genetics, 1995, 140: 745–754Google Scholar
  11. 11.
    Hua J P, Xing Y Z, Wu W R, et al. Single-locus heterotic effects and dominance by dominance interaction can adequately explain the genetic basis of heterosis in an elite hybrid. Proc Natl Acad Sci USA, 2003, 100(5): 2574–2579CrossRefGoogle Scholar
  12. 12.
    Tang J H, Teng W T, Ma X Q, et al. Genetic analysis of plant height by molecular makers using a population of recombinant inbred line in maize. Euphytica, 2006, doi: 10.1007/s10681-006-9312-3Google Scholar
  13. 13.
    Austin D F, Lee M, Veldboom L R. Genetic mapping in maize with hybrid progeny across testers and generations: Plant height and flowering. Theor Appl Genet, 2001, 102: 163–176CrossRefGoogle Scholar
  14. 14.
    Beavis W D, Grant D, Albertsen M, et al. Quantitative trait loci for plant height in four maize populations and their associations with qualitative genetic loci. Theor Appl Genet, 1991, 83: 141–145CrossRefGoogle Scholar
  15. 15.
    Berke T, Rocheford T. Quantitative trait loci for flowering, plant and ear height, and kernel traits in maize. Crop Sci, 1995, 35: 1542–1549CrossRefGoogle Scholar
  16. 16.
    Cao Y G, Wang G Y, Wang S C, et al. Construction a genetic map and location of quantitative trait loci for dwarf trait in maize by RFLP markers. Chin Sci Bull, 2000, 45(3): 247–250CrossRefGoogle Scholar
  17. 17.
    Lance R, Lee M. Genetic mapping of quantitative trait loci in maize in stress and nonstress environments: II. Plant height and flowering. Crop Sci, 1996, 36: 1320–1327CrossRefGoogle Scholar
  18. 18.
    Lin Y R, Schertz K F, Paterson A H. Comparative analysis of QTL affecting plant height and maturity across in poaceae, in reference to an interspecific sorghum population. Genetics, 1995, 141(1): 391–411Google Scholar
  19. 19.
    Veldboom L R, Lee M. Genetic mapping of quantitative trait loci in maize in stress and nonstress environments: II. Plant height and flowering. Crop Sci, 1996, 36: 1320–1327CrossRefGoogle Scholar
  20. 20.
    Lübberstedt T, Melchinger A E, Schǒon C C, et al. QTL mapping in testcrosses of European flint lines of maize I. Comparison of different testers for forage yield traits. Crop Sci, 1997, 37: 921–931CrossRefGoogle Scholar
  21. 21.
    Bensen R J, Johal G S, Crane V C, et al. Cloning and characterization of the maize An1 gene. Plant Cell, 1995, 7(1): 75–84CrossRefGoogle Scholar
  22. 22.
    Spray C R, Kobayashi M, Suzuki Y, et al. The dwarf-1(d1) mutant of Zea Mays blocks three steps in the gibberellin-biosynthetic pathway. Proc Natl Acad Sci USA, 1996, 93(19): 10515–10518CrossRefGoogle Scholar
  23. 23.
    Winkler R G, Helentjaris T. The maize dwarf3 gene encodes a cytochrome P450-mediated early step in Gibberellin biosynthesis. Plant Cell, 1995, 7(8): 1307–1317CrossRefGoogle Scholar
  24. 24.
    Yan J B, Tang H, Huang Y Q, et al. Dynamic analysis of QTL for plant height at different developmental stages in maize (Zea mays L.). Chin Sci Bull, 2003, 48(23): 1959–1964Google Scholar
  25. 25.
    Tang H, Huang Y Q, Yan J B, et al. Genetic analysis of yield traits with elite maize hybrid—Yuyu22. Acta Agron Sin (in Chinese), 2004, 30(9): 922–926Google Scholar
  26. 26.
    Lincoln S, Daly M, Lander E. Mapping genetic mapping with MAPMAKER/EXP3.0. Cambridge: Whitehead Institute Technical Report, 1992Google Scholar
  27. 27.
    Zeng Z B. Precision mapping of quantitative trait loci. Genetics, 1994, 136: 1457–1468Google Scholar
  28. 28.
    Li Z K, Luo L J, Mei H W, et al. Overdominance epistatic loci are the primary genetic basis of inbreeding depression and heterosis in rice. I. Biomass and grain yield. Genetics, 2001, 158: 1737–1753Google Scholar
  29. 29.
    Mei H W, Li Z K, Shu Q Y, et al. Gene actions of QTL affecting several agronomic traits resolved in a recombinant inbred rice population and two backcross population. Theor Appl Genet, 2005, 110: 649–659CrossRefGoogle Scholar
  30. 30.
    Xing Y Z, Tan Y F, Hua J P, et al. Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice. Theor Appl Genet, 2002, 105(2–3): 248–257Google Scholar
  31. 31.
    Yu S B, Li J X, Xu C G, et al. Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid. Proc Natl Acad Sci USA, 1997, 94: 9226–9231CrossRefGoogle Scholar

Copyright information

© Science in China Press 2007

Authors and Affiliations

  • Tang JiHua 
    • 1
    • 2
  • Ma XiQing 
    • 1
  • Teng WenTao 
    • 1
  • Yan JianBing 
    • 1
  • Wu WeiRen 
    • 3
  • Dai JingRui 
    • 1
  • Li JianSheng 
    • 1
  1. 1.National Maize Improvement Center of ChinaChina Agricultural UniversityBeijingChina
  2. 2.College of AgronomyHenan Agricultural UniversityZhengzhouChina
  3. 3.Institute of BioinformaticsZhejiang UniversityHangzhouChina

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