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Exogenous cAMP upregulates the expression of glnII and glnK-amtB genes in Sinorhizobium meliloti 1021

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Chinese Science Bulletin

Abstract

The existence of multiple adenylate cyclase encoding genes implies the importance of cAMP in Sinorhizobium meliloti 1021. In this study, as a pioneer step of understanding cAMP roles, microarray analysis on S. meliloti was carried out for the function of exogenous cAMP. To our surprise, the result showed that the transcriptions of glnII and glnK genes were significantly upshifted in the presence of exogenous cAMP in S. meliloti. This phenomenon is further confirmed in S. meliloti that the expression of either glnII or glnK promoter-lacZ translational fusion is higher in the presence of exogenous cAMP. Therefore, for the first time, we have identified genes from S. meliloti whose expression is activated by cAMP. The potential physiological role of upregulation of glnII and glnK by cAMP is discussed.

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Authors and Affiliations

  1. National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871, China

    Tian Zhexian, Mao Xianjun, Su Wei, Li Jian & Wang Yiping

  2. Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, 33501, Bielefeld, Germany

    Becker Anke

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  1. Tian Zhexian
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  2. Mao Xianjun
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  3. Su Wei
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  4. Li Jian
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  5. Becker Anke
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  6. Wang Yiping
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Correspondence to Wang Yiping.

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Tian, Z., Mao, X., Su, W. et al. Exogenous cAMP upregulates the expression of glnII and glnK-amtB genes in Sinorhizobium meliloti 1021. CHINESE SCI BULL 51, 1982–1985 (2006). https://doi.org/10.1007/s11434-006-2079-z

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  • DOI: https://doi.org/10.1007/s11434-006-2079-z

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