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Probe stiffness regulates receptor-ligand bond lifetime under force

  • Yan Zhang
  • ShouQin Lü
  • Mian Long
Research Paper

Abstract

Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5×10−3 to 4.7×10−2 pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k⩾3.0×10−2 pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow.

Keywords

probe stiffness bond lifetime optical trap 

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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.Key Laboratory of Microgravity, Institute of MechanicsChinese Academy of SciencesBeijingChina
  2. 2.National Microgravity Laboratory and Center of Biomechanics and Bioengineering, Institute of MechanicsChinese Academy of SciencesBeijingChina

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