Science in China Series F

, Volume 49, Issue 3, pp 397–408 | Cite as

A micro amperometric immunosensor for detection of human immunoglobulin

  • Xu Yuanyuan 
  • Xia Shanhong 
  • Bian Chao 
  • Chen Shaofeng 
Article

Abstract

A novel amperometric immunosensor based on the micro electromechanical systems (MEMS) technology, using protein A and self-assembled monolayers (SAMs) for the orientation-controlled immobilization of antibodies, has been developed. Using MEMS technology, an “Au, Pt, Pt” three-microelectrode system enclosed in a SU-8 micro pool was fabricated. Employing SAMs, a monolayer of protein A was immobilized on the cysteamine modified Au electrode to achieve the orientation-controlled immobilization of the human immunoglobulin (HIgG) antibody. The immunosensor aimed at low unit cost, small dimension, high level of integration and the prospect of a biosensor system-on-a-chip. Cyclic voltammetry and chronoamperometry were conducted to characterize the immunosensor. Compared with the traditional immunosensor using bulky gold electrode or screen-printed electrode and the procedure directly binding protein A to electrode for immobilization of antibodies, it had attractive advantages, such as miniaturization, compatibility with CMOS technology, fast response (30 s), broad linear range (50–400 µg/L) and low detection limit (10 μg/L) for HIgG. In addition, this immunosensor was easy to be designed into micro array and to realize the simultaneously multi-parameter detection.

Keywords

amperometric immunosensor micro electromechanical systems (MEMS) self-assembled monolayers (SAMs) protein A orientation-controlled immobilization biosensor system-on-a-chip 

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Copyright information

© Science in China Press 2006

Authors and Affiliations

  • Xu Yuanyuan 
    • 1
  • Xia Shanhong 
    • 1
  • Bian Chao 
    • 1
  • Chen Shaofeng 
    • 1
  1. 1.State Key Laboratory of Transducer Technology, Institute of ElectronicsChinese Academy of SciencesBeijingChina

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