Science China Technological Sciences

, Volume 58, Issue 2, pp 273–283 | Cite as

Transparent soft PDMS eggshell

Article

Abstract

In vivo 3D fluorescent image remains a technological barrier for biologists and clinical scientists although green fluorescent protein (GFP) imaging has long been performed rather well at cellular level. Meanwhile, robust enough portable devices are also challenging lab-on-a-chip advocators who wish their designs to be nurtured by the end users. This work is dedicated to propose a conceptually innovated transparent soft PDMS avian eggshell to directly tackle the above two goals. Here, an “egg-on-a-chip” scheme is originally developed and demonstrated by a newly developed PDMS “soft” process method. Unlike its ancestor-the conventional “lab-on-a-chip” (LOC) which is basically chemically based, the current “egg-on-a-chip”, intrinsically inherited with biological natures, opens a way to integrate biological parts or whole system in a miniature sized device. Such biomimics system contains much condensed environmental evolutional tensor inside than those of the existing LOC compacted with artificial components which however are quite difficult to incorporate various life factors inside. Owning unique advantages, a series of transparent PDMS whole “eggshells” have been fabricated and applied to culture avian embryos up to 17.5 days and chimeric eggshells were engineered on normal eggs. In addition, X-stage embryos were successfully initiated in such system and pre-chorioallantoic membrane was observed. Further, limitation of the present process was interpreted and potential approach to improve it was suggested. With both high optical transparency and engineering subtlety fully integrated together, the present method not only provides an ideal transparent imaging platform for studying functional embryo development including life mystery, but also promises a future strategy for “lab-on-an-egg” technology which may be important in a wide variety of either fundamental or practical areas.

Keywords

soft PDMS process method biological mould egg-on-a-chip portable device pre-chorioallantoic membrane biomimics 

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References

  1. 1.
    Karumuri S R, Srinivas Y, Sekhar J V, et al. Review on break through MEMS Technology. Arch Phy Res, 2011, 4: 158–165Google Scholar
  2. 2.
    Tantra R, Jarman J. μTAS (micro total analysis systems) for the high-throughput measurement of nanomaterial solubility. J Phys Conf Series, 2013, 429: 012011CrossRefGoogle Scholar
  3. 3.
    Stavis S M. A glowing future for lab on a chip testing standards. Lab Chip, 2012, 12: 3008–3011CrossRefGoogle Scholar
  4. 4.
    Daw R, Finkelstein J. Lab on a chip. Nature, 2006, 442, 7101CrossRefGoogle Scholar
  5. 5.
    Bélanger M C, Marois Y. Hemocompatibility, biocompatibility, inflammatory and in vivo studies of primary reference materials low-density polyethylene and polydimethylsiloxane: A review. J Biomed Mater Res, 2001, 58: 467–477CrossRefGoogle Scholar
  6. 6.
    Piruska A, Nikcevic I, Lee S H, et al. The autofluorescence of plastic materials and chips measured under laser irradiation. J Lab Chip, 2005, 5: 1348–1354CrossRefGoogle Scholar
  7. 7.
    Romanowsky M B, Heymann M, Abate A R, et al. Functional patterning of PDMS microfluidic devices using integrated chemo-masks. Lab Chip, 2010, 10: 1521–1524CrossRefGoogle Scholar
  8. 8.
    Sollier E, Murray C, Maoddi P, et al. Rapid prototyping polymers for microfluidic devices and high pressure injections. Lab Chip, 2011, 11: 3752–3765CrossRefGoogle Scholar
  9. 9.
    Merkel T C, Bondar V I, Nagai K, et al. Gas sorption, diffusion, and permeation in poly (dimethylsiloxane). J Polym Sci B Polym Phys, 2000, 38: 415–434CrossRefGoogle Scholar
  10. 10.
    Zhou J W, Ellis A V, Voelcker N H. Recent developments in PDMS surface modification for microfluidic devices. Electrophoresis, 2010, 31: 2–16CrossRefGoogle Scholar
  11. 11.
    Au A K, Lai H, Utela B R, et al. Microvalves and micropumps for bioMEMS. Micromachines, 2011, 2: 179–220CrossRefGoogle Scholar
  12. 12.
    Choi J S, Piao Y, Seo T S. Fabrication of a circular PDMS microchannel for constructing a three-dimensional endothelial cell layer. Bioprocess Biosyst Eng, 2013, 36: 1871–1878CrossRefGoogle Scholar
  13. 13.
    Huh D, Leslie D C, Matthews B D, et al. A human disease model of drug toxicity-induced pulmonary edema in a lung-on-a-chip microdevice. Sci Transl Med, 2012, 4: 147–159CrossRefGoogle Scholar
  14. 14.
    Grosberg A, Alford P W, McCain M L, et al. Ensembles of engi neered cardiac tissues for physiological and pharmacological study: Heart on a chip. Lab Chip, 2011, 11: 4165–4173CrossRefGoogle Scholar
  15. 15.
    Kim H J, Huh D, Hamiltona G, et al. Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow. Lab Chip, 2012, 12: 2165–2174CrossRefGoogle Scholar
  16. 16.
    Lee S A, No D Y, Kang E, et al. Spheroid-based three-dimensional liver-on-a-chip to investigate hepatocyte-hepatic stellate cell interactions and flow effects. Lab Chip, 2013, 13: 3529–3537CrossRefGoogle Scholar
  17. 17.
    Jang K J, Suh K Y. A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells. Lab Chip, 2010, 10: 36–42CrossRefGoogle Scholar
  18. 18.
    Torisawa Y S, Spina C S, Collins J J, et al. Bone marrow-on-a-chip. In: 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2012. 563–565Google Scholar
  19. 19.
    Huang Y, Williams J C, Johnson S M. Brain slice on a chip: Opportunities and challenges of applying microfluidic technology to intact tissues. Lab Chip, 2012, 12: 2103–2117CrossRefGoogle Scholar
  20. 20.
    Prabhakarpandian B, Shen M C, Nichols J B, et al. SyM-BBB: A microfluidic blood brain barrier model. Lab Chip, 2013, 6: 1093–1101CrossRefGoogle Scholar
  21. 21.
    Leslie D C, Domansky K, Hamilton G A, et al. Aerosol drug delivery for lung on a chip. In: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2011. 97–99Google Scholar
  22. 22.
    Ataç B, Wagner I, Horland R, et al. Skin and hair on-a-chip: In vitro skin models versus ex vivo tissue maintenance with dynamic perfusion. Lab Chip, 2013, 13: 3555–3561.CrossRefGoogle Scholar
  23. 23.
    Baker M. A living system on a chip. Nature, 2011, 471: 661–665.CrossRefGoogle Scholar
  24. 24.
    Williamson A, Singh S, Fernekorn U, et al. The future of the patient-specific body-on-a-chip. Lab Chip, 2013, 13: 3471–3480CrossRefGoogle Scholar
  25. 25.
    Marx U, Walles H, Hoffmann S, et al. ‘Human-on-a-chip’ developments: A translational cutting edge alternative to systemic safety assessment and efficiency evaluation of substances in laboratory animals and man? ATLA, 2012, 40: 235–257Google Scholar
  26. 26.
    Burggren W W. What is the purpose of the embryonic heart beat? or How facts can ultimately prevail over physiological dogma. Physiol Biochem Zool, 2004, 77: 333–345CrossRefGoogle Scholar
  27. 27.
    Hopwood N. Producing development: The anatomy of human embryos and the norms of Wilhelm His. Bull Hist Med, 2000, 74: 29–79CrossRefGoogle Scholar
  28. 28.
    White R M, Sessa A, Burke C, et al. Transparent adult zebrafish as a tool for in vivo transplantation analysis. Cell Stem Cell, 2008, 2: 183–189CrossRefGoogle Scholar
  29. 29.
    Levine A J, Munoz-Sanjuan I, Bell E, et al. Fluorescent labeling of endothelial cells allows in vivo, continuous characterization of the vascular development of Xenopus laevis. Dev Biol, 2003, 254: 50–67CrossRefGoogle Scholar
  30. 30.
    Bach E A, Ekas L A, Ayala-Camargo A, et al. GFP reporters detect the activation of the drosophila JAK/STAT pathway in vivo. Gene Exp Pattern, 2007, 7: 323–331CrossRefGoogle Scholar
  31. 31.
    Lim E, Modi K D, Kim J. In vivo bioluminescent imaging of mammary tumors using IVIS spectrum. JoVE, 2009, 26: 1–2Google Scholar
  32. 32.
    Allison R R, Mota H C, Sibata C H. Clinical PD/PDT in north America: An historical review. Photodiag Photodyn Therapy, 2004, 1: 263–277CrossRefGoogle Scholar
  33. 33.
    Ragan T, Kadiri L R, Venkataraju K U, et al. Serial two-photon tomography for automated ex vivo mouse brain imaging. Nat Methods, 2012, 9: 255–258CrossRefGoogle Scholar
  34. 34.
    Tufan A C, Akdogan I, Adiguzel E. Shell-less culture of the chick embryo as a model system in the study of developmental neurobiology. Neuroanatomy, 2004, 3: 8–11Google Scholar
  35. 35.
    Dohle D S, Pasa S D, Gustmann S, et al. Chick ex ovo culture and ex ovo CAM assay: How it really works. J Visualized Exp, 2009, 33: 1–8Google Scholar
  36. 36.
    Yalcin H C, Shekhar A, Rane A A, et al. An ex-ovo chicken embryo culture system suitable for imaging and microsurgery applications. JoVE, 2010, 44: 1–5Google Scholar
  37. 37.
    Schomann T, Qunneis F, Widera D, et al. Improved method for ex ovo-cultivation of developing chicken embryos for human stem cell Xenografts. Stem Cells Int, 2013, 2013: 960958CrossRefGoogle Scholar
  38. 38.
    Chapman S C, Collignon J, Schoenwolf G C, et al. Improved method for chick whole-embryo culture using a filter paper Carrier. Dev Biol, 2001, 220: 284–289Google Scholar
  39. 39.
    Toepke M W, Beebe D J. PDMS absorption of small molecules and consequences in microfluidic applications. Lab Chip, 2006, 6: 1484–1486CrossRefGoogle Scholar
  40. 40.
    Keller R. Cell migration during gastrulation. Curr Opin Cell Biol, 2005, 17: 533–541CrossRefGoogle Scholar
  41. 41.
    Dormann D, Weijer C J. Imaging of cell migration. EMBO J, 2006, 25: 3480–3493CrossRefGoogle Scholar
  42. 42.
    Kulesa P M, Fraser S E. In ovo time-lapse analysis of chick hindbrain neural crest cell migration shows cell interactions during migration to the branchial arches. Development, 2000, 127: 1161–1172Google Scholar
  43. 43.
    Griswold S L, Lwigale P Y. Analysis of neural crest migration and differentiation by cross-species transplantation. JoVE, 2012, 60: 1–8Google Scholar
  44. 44.
    Naito M, Sano A, Matsubara Y, et al. Localization of primordial germ cells or their precursors in stage X blastoderm of chickens and their ability to differentiate into functional gametes in opposite-sex recipient gonads. Reproduction, 2001, 121: 547–552CrossRefGoogle Scholar
  45. 45.
    Richardson B E, Lehmann R. Mechanisms guiding primordial germ cell migration: strategies from different organisms. Nat Rev Mol Cell Bio, 2010, 11: 37–49CrossRefGoogle Scholar
  46. 46.
    Stebler J, Spieler D, Slanchev K, et al. Primordial germ cell migration in the chick and mouse embryo: The role of the chemokine SDF-1/CXCL12. Dev Biol, 2004, 272: 351–361CrossRefGoogle Scholar
  47. 47.
    Hen G, Friedman-Einat M, Sela-Donenfeld D. Primordial germ cells in the dorsal mesentery of the chicken embryo demonstrate left-right asymmetry and polarized distribution of the EMA1 epitope. J Anat 2014, 224: 556–563CrossRefGoogle Scholar
  48. 48.
    McLennan R, Dyson L, Prather K W, et al. Multiscale mechanisms of cell migration during development: Theory and experiment. Development, 2012, 139: 2935–2944CrossRefGoogle Scholar
  49. 49.
    Bernardo A M, Sprenkels A, Rodrigues G, et al. Chicken primordial germ cells use the anterior vitelline veins to enter the embryonic circulation. Biology Open, 2012, 1: 1146–1152CrossRefGoogle Scholar
  50. 50.
    Hamburger V, Hamilton H L. A series of normal stages in the development of chicken embryo. Devel Dyn, 1992, 195: 231–272CrossRefGoogle Scholar

Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of Biomedical Engineering, School of MedicineTsinghua UniversityBeijingChina
  2. 2.Beijing Key Laboratory of CryoBiomedical Engineering and Key Laboratory of Cryogenics, Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijingChina

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