Dopamine detection using a patch-clamp system on a planar microeletrode array electrodeposited by polypyrrole/graphene nanocomposites
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To achieve a dopamine (DA) response with high sensitivity and high signal-to-noise ratio (S/N) with a patch-clamp system, polypyrrole/graphene (PPy/GR) nanocomposites were steadily electrodeposited by an electrochemical method on a planar microelectrode array (pMEA) fabricated by a standard micromachining process. The electrodeposition process was carried out by chronopotentimetry measurement scanning from 0.1 to 0.8 C/cm2 at the current of 2 mA; 0.5 C/cm2 was found to be optimal. The pMEA modified by PPy/GR at the 0.5 C/cm2 exhibits remarkable properties; for instance, the standard deviation (SD) decreases from 8.4614×10−11 to 5.62×10−11 A, reduced by 33.52%, and the sensitivity increases from 2566.88 to 76114.65 μAmMcm−2, 29.65 times higher than the bare Pt (platinum). A good linear relationship between the current and DA concentration in the range of 0.30 to 61.71 μm was obtained, with a correlation coefficient of 0.997. The sensor is meaningful for neuroscience research and the treatment of neurological diseases.
Keywordspolypyrrole/graphene planar microelectrode array electrodeposition dopamine
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- 4.Fiona M N, Andrea V, Lars F. Changes in cortical dopamine d1 re ceptor binding associated with cognitive training. Science, 323: 800–802Google Scholar
- 6.Zhou G Z, Wang D, Xu S F. Effect if dopamine system in caudate nucleus on acupuncture analgesia. Chin Sci Bull, 1981, 26: 1043–1047.Google Scholar
- 7.Satish K, Harit D, Mohan S, et al. Polymer Communication Fibers from polypropylene/nano carbon fiber composites. Polymer, 43: 1701–1703Google Scholar
- 13.Wang H, Cai H R, Tai H J. Modulation of presynaptic nAChRs on postsynaptic GABA receptor in optic tectum of juvenile Xenopus Chin Sci Bull, 2003, 48: 417–423Google Scholar
- 18.Nair R R, Wu H A, Jayaram P N, et al. Unimpeded permeation of water through helium-leak-tight graphene-based membrane. Science, 335: 442–444Google Scholar
- 19.Zhuang Z J, Li J Y, Xu R, et al. Electrochemical detection of dopamine in the presence of ascorbic acid using overoxidized polypyrrole/graphene modified electrodes. Inter J Electrochem Sci, 2011, 1: 2149–2161Google Scholar
- 23.Hou S F, Kasner M L., Su S J, Highly sensitive and selective dopamine biosensor fabricated with silanized graphene. J Phys Chem, 2010, 114: 14915–14921Google Scholar
- 24.Heike E. A brain potential study of preparation for and execution of a task-switch with stimuli that afford only the relevant task. Neuroscience, 2010, 33: 1137–1154Google Scholar
- 25.Barizuddin S, Liu X, Mathai J C, et al. Automated targeting of cells to electrochemical electrodes using a surface chemistry approach for the measurement of quantal exocytosis. Neuroscience, 2010, 1: 590–597Google Scholar