Recently duplicated sesterterpene (C25) gene clusters in Arabidopsis thaliana modulate root microbiota
Land plants co-speciate with a diversity of continually expanding plant specialized metabolites (PSMs) and root microbial communities (microbiota). Homeostatic interactions between plants and root microbiota are essential for plant survival in natural environments. A growing appreciation of microbiota for plant health is fuelling rapid advances in genetic mechanisms of controlling microbiota by host plants. PSMs have long been proposed to mediate plant and single microbe interactions. However, the effects of PSMs, especially those evolutionarily new PSMs, on root microbiota at community level remain to be elucidated. Here, we discovered sesterterpenes in Arabidopsis thaliana, produced by recently duplicated prenyltransferase-terpene synthase (PT-TPS) gene clusters, with neo-functionalization. A single-residue substitution played a critical role in the acquisition of sesterterpene synthase (sesterTPS) activity in Brassicaceae plants. Moreover, we found that the absence of two root-specific sesterterpenoids, with similar chemical structure, significantly affected root microbiota assembly in similar patterns. Our results not only demonstrate the sensitivity of plant microbiota to PSMs but also establish a complete framework of host plants to control root microbiota composition through evolutionarily dynamic PSMs.
Enplant specialized metabolites microbiota sesterterpene terpene synthase
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This work was supported by the Priority Research Program of the Chinese Academy of Sciences (ZDRW-ZS-2019-2 and QYZDB-SSW-SMC021), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA08000000 and XDB11020700), the National Program on Key Basic Research Projects (2013CB127000), and the State Key Laboratory of Plant Genomics of China (2016A0219-11 and SKLPG2013A0125-5). We thank Dr. Jay D Keasling (University of California, Berkeley) for providing the pMBIS plasmid.
- Benjamini, Y., and Hochberg, Y. (1995). Controlling the false discovery rate: A practical and powerful approach to multiple testing. J R Statist Soc-Ser B 57, 289–300.Google Scholar
- Kampranis, S.C., Ioannidis, D., Purvis, A., Mahrez, W., Ninga, E., Katerelos, N.A., Anssour, S., Dunwell, J.M., Degenhardt, J., Makris, A. M., et al. (2007). Rational conversion of substrate and product specificity in a Salvia monoterpene synthase: Structural insights into the evolution of terpene synthase function. Plant Cell 19, 1994–2005.CrossRefGoogle Scholar
- Zhao, T., Holmer, R., de Bruijn, S., Angenent, G.C., van den Burg, H.A., and Schranz, M.E. (2011). Phylogenomic synteny network analysis of MADS-box transcription factor genes reveals lineage-specific transpositions, ancient tandem duplications, and deep positional conservation. Plant Cell 29, 1278–1292.Google Scholar