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Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants

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References

  1. Čermák, T., Curtin, S.J., Gil-Humanes, J., Čegan, R., Kono, T.J.Y., Konečná, E., Belanto, J.J., Starker, C.G., Mathre, J.W., Greenstein, R. L., et al. (2017). A multipurpose toolkit to enable advanced genome engineering in plants. Plant Cell 29, 1196–1217.

  2. Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P.D., Wu, X., Jiang, W., Marraffini, L.A., et al. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819–823.

  3. Connelly, S., Marshallsay, C., Leader, D., Brown, J.W., and Filipowicz, W. (1994). Small nuclear RNA genes transcribed by either RNA polymerase II or RNA polymerase III in monocot plants share three promoter elements and use a strategy to regulate gene expression different from that used by their dicot plant counterparts. Mol Cell Biol 14, 5910–5919.

  4. Ding, D., Chen, K., Chen, Y., Li, H., and Xie, K. (2018). Engineering introns to express RNA guides for Cas9- and Cpf1-mediated multiplex genome editing. Mol Plant 11, 542–552.

  5. Engler, C., Kandzia, R., and Marillonnet, S. (2008). A one pot, one step, precision cloning method with high throughput capability. PLoS ONE 3, e3647.

  6. Li, G., Liu, Y.G., and Chen, Y. (2019). Genome-editing technologies: the gap between application and policy. Sci China Life Sci 62, 1534–1538.

  7. Liu, W., Xie, X., Ma, X., Li, J., Chen, J., and Liu, Y.G. (2015). DSDecode: A web-based tool for decoding of sequencing chromatograms for genotyping of targeted mutations. Mol Plant 8, 1431–1433.

  8. Ma, X., Zhang, Q., Zhu, Q., Liu, W., Chen, Y., Qiu, R., Wang, B., Yang, Z., Li, H., Lin, Y., et al. (2015). A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant 8, 1274–1284.

  9. Xie, K., Minkenberg, B., and Yang, Y. (2015). Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci USA 112, 3570–3575.

  10. Xie, X., Ma, X., Zhu, Q., Zeng, D., Li, G., and Liu, Y.G. (2017). CRISPR-GE: A convenient software toolkit for CRISPR-based genome editing. Mol Plant 10, 1246–1249.

  11. Zeng D., Ma, X., Xie, X., Zhu, Q., and Liu, YG. (2018). A protocol for CRISPR/Cas9-based multi-gene editing and sequence decoding of mutant sites in plants (in Chinese). Sci Sin Vitae 48, 783–794.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (91435203 and 31991222) and the Major Program of Guangdong Basic and Applied Basic Research (2019B030302006).

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Correspondence to Jingxin Guo or Yao-Guang Liu.

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The author(s) declare that they have no conflict of interest.

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Hao, Y., Zong, W., Zeng, D. et al. Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants. Sci. China Life Sci. (2020). https://doi.org/10.1007/s11427-019-1612-6

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