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Development of an innovative one-step nested PCR strategy for virus detection using the LNA technique

  • Ji Wang
  • Li Zhao
  • Zhihua Sun
  • Guixia Li
  • Peihua Niu
  • Dandi Li
  • Lianjun Wang
  • Yi Zhang
  • Zhishan Feng
  • Xuejun Ma
Open Access
Letter to the Editor
  • 4 Downloads

Notes

Acknowledgements

This work was supported by the National Key Research and Development Plan (2016TFC1202700, 2016YFC1200900), Beijing Municipal Science & Technology Commission Project (D151100002115003) and Guangzhou Municipal Science & Technology Commission project (2015B2150820).

References

  1. Chieochansin, T., Chutinimitkul, S., Payungporn, S., Hiranras, T., Samransamruajkit, R., Theamboolers, A., and Poovorawan, Y. (2007). Complete coding sequences and phylogenetic analysis of human bocavirus (HBoV). Virus Res 129, 54–57.CrossRefGoogle Scholar
  2. Ge, S., Yan, Q., He, S., Zhuang, S., Niu, J., and Xia, N. (2013). Specific primer amplification of the VP1 region directed by 5′ UTR sequence analysis: enterovirus testing and identification in clinical samples from hand-foot-and-mouth disease patients. J Virol Methods 193, 463–469.CrossRefGoogle Scholar
  3. World Health Organization. (2009). Manual of rotavirus detection and characterization methods. South African Med J 99, 245–246.Google Scholar

Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ji Wang
    • 1
  • Li Zhao
    • 1
    • 2
  • Zhihua Sun
    • 3
  • Guixia Li
    • 5
  • Peihua Niu
    • 1
  • Dandi Li
    • 1
  • Lianjun Wang
    • 4
  • Yi Zhang
    • 1
  • Zhishan Feng
    • 5
  • Xuejun Ma
    • 1
  1. 1.National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina
  2. 2.Hebei Medical UniversityShijiazhuangChina
  3. 3.Beijing Chaoyang Maternal & Child Health HospitalBeijingChina
  4. 4.Dongcheng Center for Disease Control and PreventionBeijingChina
  5. 5.Children’s Hospital of Hebei ProvinceShijiazhuangChina

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